An RGA – like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat

2001 ◽  
Vol 103 (6-7) ◽  
pp. 1007-1013 ◽  
Author(s):  
L. Huang ◽  
B. S. Gill
Keyword(s):  
Gene Family ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Aegilops Tauschii ◽  
Family Members ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family

2007 ◽  
Vol 65 (1-2) ◽  
pp. 93-106 ◽  
Author(s):  
Sylvie Cloutier ◽  
Brent D. McCallum ◽  
Caroline Loutre ◽  
Travis W. Banks ◽  
Thomas Wicker ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Gene Family ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

scholarly journals Molecular analyses in wheat and Aegilops tauschii reveals a new orthologue of the leaf rust resistance gene Lr19 on 7DL chromosome of Ae. tauschii

2021 ◽  
Author(s):  
Zahra Pourkhorshid ◽  
Ali Dadkhodaie ◽  
Roohollah Shamloo-Dashtpagerdi‎
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Aegilops Tauschii ◽  
Leaf Rust Resistance ◽  
Bioinformatic Analysis ◽  
Genomic Region ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Wheat Diseases

Abstract Leaf rust is one of the most devastating wheat diseases worldwide, to which many resistance genes have been ‎successfully introgressed ‎from wheat wild relatives. Though the Thinopyrum ‎ponticum-derived leaf rust resistance gene Lr19, is widely effective worldwide and previous studies ‎have shown its likely presence in Aegilops tauschii, no ‎thorough investigation has been conducted to confirm this. The present study aimed to ‎examine the presence of Lr19 in Ae. tauschii using a collection of molecular and bioinformatic analysis. Accordingly, the Thatcher line was used as susceptible, and a Thatcher+Lr19 (TcLr19) and Agatha were used as resistant lines. CDHLQ pathotyping coupled with DNA markers genotyping verified the presence of an Lr19 orthologue on Ae. tauschii 7DL (AtLr19). Sequencing of the GB marker products from Ae. tauschii and TcLr19 showed 99% homology in these fragments, confirming phenotyping and genotyping results. Both isolated segments were matched to a putative melatonin biosynthesis gene, namely O-methyltransferase-2 (OMT2) mapped to 7DL, with 100% identity. A hierarchical gene network was reconstructed using all identified putative genes within a genomic region containing 2.5 cM upstream and downstream of the OMT2 gene. Results indicated that several numbers of important biotic stress-responsive genes such as RPM1, RGA2, TRIUR3, BURP12, and myosin-11, were located downstream of melatonin as a master regulator molecule through the OMT2 node. To our knowledge, this is the first report of finding an orthologue for ‎Lr19 in Ae. tauschii, which provides insights into the possible regulatory route of LR19.

scholarly journals Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Agronomic Traits ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.

scholarly journals Microsatellite markers for the Triticum timopheevi-derived leaf rust resistance gene Lr18 on wheat 5BL chromosome

2017 ◽  
Vol 67 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Ali Aliakbari Sadeghabad ◽  
Ali Dadkhodaie ◽  
Bahram Heidari ◽  
Hooman Razi ◽  
Reza Mostowfizadeh-Ghalamfarsa
Keyword(s):  
Microsatellite Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Triticum Timopheevi

Molecular Mapping of Leaf Rust Resistance Gene 5 in Barley

Crop Science ◽  
2003 ◽  
Vol 43 (1) ◽  
pp. 388 ◽  
Author(s):  
J. A. Mammadov ◽  
J. C. Zwonitzer ◽  
R. M. Biyashev ◽  
C. A. Griffey ◽  
Y. Jin ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene
Sign in / Sign up

Export Citation Format

Share Document