Flow cytometric sorting of maize chromosome 9 from an oat-maize chromosome addition line

2001 ◽  
Vol 102 (5) ◽  
pp. 658-663 ◽  
Author(s):  
L. J. Li ◽  
K. Arumuganathan ◽  
H. W. Rines ◽  
R. L. Phillips ◽  
O. Riera-Lizarazu ◽  
...  
Keyword(s):  
Chromosome 9 ◽  
Addition Line ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Flow Cytometric ◽  
Flow Cytometric Sorting ◽  
Chromosome Addition Line

scholarly journals Production and Characterization of Maize Chromosome 9 Radiation Hybrids Derived From an Oat-Maize Addition Line

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 327-339 ◽  
Author(s):  
O Riera-Lizarazu ◽  
M I Vales ◽  
E V Ananiev ◽  
H W Rines ◽  
R L Phillips
Keyword(s):  
Radiation Hybrid ◽  
Chromosome Region ◽  
Chromosome 9 ◽  
Addition Line ◽  
Addition Lines ◽  
Organization Studies ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Radiation Hybrids ◽  
Chromosome Addition Lines

Abstract In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)—oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0.5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.

scholarly journals Complex Structure of Knob DNA on Maize Chromosome 9: Retrotransposon Invasion into Heterochromatin

Genetics ◽  
1998 ◽  
Vol 149 (4) ◽  
pp. 2025-2037 ◽  
Author(s):  
E V Ananiev ◽  
R L Phillips ◽  
H W Rines
Keyword(s):  
Dna Sequences ◽  
Complex Structure ◽  
Chromosome 9 ◽  
Addition Line ◽  
Addition Lines ◽  
Repeated Dna ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Retrotransposable Elements ◽  
Chromosome Addition Lines

Abstract The recovery of maize (Zea mays L.) chromosome addition lines of oat (Avena sativa L.) from oat × maize crosses enables us to analyze the structure and composition of specific regions, such as knobs, of individual maize chromosomes. A DNA hybridization blot panel of eight individual maize chromosome addition lines revealed that 180-bp repeats found in knobs are present in each of these maize chromosomes, but the copy number varies from ~100 to 25,000. Cosmid clones with knob DNA segments were isolated from a genomic library of an oat-maize chromosome 9 addition line with the help of the 180-bp knob-associated repeated DNA sequence used as a probe. Cloned knob DNA segments revealed a complex organization in which blocks of tandemly arranged 180-bp repeating units are interrupted by insertions of other repeated DNA sequences, mostly represented by individual full size copies of retrotransposable elements. There is an obvious preference for the integration of retrotransposable elements into certain sites (hot spots) of the 180-bp repeat. Sequence microheterogeneity including point mutations and duplications was found in copies of 180-bp repeats. The 180-bp repeats within an array all had the same polarity. Restriction maps constructed for 23 cloned knob DNA fragments revealed the positions of polymorphic sites and sites of integration of insertion elements. Discovery of the interspersion of retrotransposable elements among blocks of tandem repeats in maize and some other organisms suggests that this pattern may be basic to heterochromatin organization for eukaryotes.

A maize chromosome 3 addition line of oat exhibits expression of the maize homeobox gene liguleless3 and alteration of cell fates

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1055-1064 ◽  
Author(s):  
G J Muehlbauer ◽  
O Riera-Lizarazu ◽  
R G Kynast ◽  
D Martin ◽  
R L Phillips ◽  
...  
Keyword(s):  
Cell Fate ◽  
Ectopic Expression ◽  
Homeobox Gene ◽  
Chromosome 3 ◽  
Addition Line ◽  
Addition Lines ◽  
Morphological Examination ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Chromosome Addition Lines

Maize chromosome addition lines of oat offer the opportunity to study maize gene expression in oat and the resulting phenotypes. Morphological examination of a maize chromosome 3 addition line of oat showed that this line exhibited several morphological abnormalities including a blade-to-sheath transformation at the midrib region of the leaf, a hook-shaped panicle, and abnormal outgrowth of aerial axillary buds. Dominant mutations in the maize liguleless3 (lg3) homeobox gene result in a blade (distal)-to-sheath (proximal) transformation at the midrib region of the leaf. Ectopic expression of the dominant mutant Lg3 allele is believed to cause the phenotype. Therefore, we suspected that the maize lg3 gene, which is located on maize chromosome 3, was involved in the phenotypes observed in the maize chromosome 3 addition line of oat. Genetic analyses of an oat BC1F2 family segregating for maize chromosome 3 showed that the presence of a stable maize chromosome 3 was required for the expression of these cell fate abnormalities. RNA expression analysis of leaf sheath tissue from oat plants carrying maize chromosome 3 demonstrated that maize LG3 transcripts accumulated in oat, indicating that this expression is associated with the blade-to-sheath transformation, hook-shaped panicle and outgrowth of aerial axillary bud phenotypes. Our results demonstrate that the maize chromosome addition lines of oat are useful genetic stocks to study expression of maize genes in oat.Key words: liguleless3, homeobox, oat-maize addition line.

scholarly journals Occurrence of hordatines, the barley antifungal colnpounds, in a wheat-barley chromosome addition line.

1999 ◽  
Vol 74 (3) ◽  
pp. 99-103 ◽  
Author(s):  
Taiji Nomura ◽  
Masayuki Sue ◽  
Ryo Horikoshi ◽  
Shin-ichi Tebayashi ◽  
Atsushi Ishihara ◽  
...  
Keyword(s):  
Barley Chromosome ◽  
Addition Line ◽  
Chromosome Addition ◽  
Chromosome Addition Line

scholarly journals Targeted sequencing of the short arm of chromosome 6V of a wheat relative Haynaldia villosa for marker development and gene mining

2020 ◽  
Author(s):  
Jin Xiao ◽  
Wentao Wan ◽  
Mengli Li ◽  
Zhongyu Yu ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Aegilops Tauschii ◽  
Wheat Breeding ◽  
Linkage Drag ◽  
Addition Line ◽  
Sequencing Data ◽  
Haynaldia Villosa ◽  
Breeding Programs ◽  
Homologous Genes ◽  
Flow Cytometric ◽  
Flow Cytometric Sorting

Abstract Background: Short arm of chromosome 6V (6VS) of Haynaldia villosa has been used in wheat breeding programs to introduce Pm21 resistance gene against powdery mildew and some other genes. Results: In this work, 6VS was isolated from a wheat ( Triticum aestivum ) - 6VS telosome addition line by flow cytometric sorting and sequenced by illumina technology. The assembly length was 230.39 Mb with contig N50 of 9,788 bp. The sequence annotation identified 3,276 high confidence genes supported by RNA sequencing data, representing about 2.3% of the chromosome arm sequence; repetitive elements accounted for 74.91% of the arm sequence. Sequences homologous to 6VS genes were identified on short arms of chromosomes 6A of T. urartu , 6D of Aegilops tauschii , 6A and 6B of T. dicoccoides , 6A, 6B and 6D of T. aestivum and 6H of Hordeum vulgare , revealing synteny relationships among these chromosome arms. Based on differences in intron size between the homologous genes on 6VS and 6AS/6BS/6DS of T. aestivum , 222 primer pairs were designed. Out of them, 120 amplified 6VS-specific products and are suitable as intron-target (IT) markers to trace the 6VS chromatin introduced into wheat. Conclusions: The results obtained and markers developed in this work will facilitate introduction of important genes to common wheat from its wild relative, while reducing the presence of unfavorable genes due to linkage drag.

scholarly journals Targeted sequencing of the short arm of chromosome 6V of a wheat relative Haynaldia villosa for marker development and gene mining

2020 ◽  
Author(s):  
Jin Xiao ◽  
Wentao Wan ◽  
Mengli Li ◽  
Zhongyu Yu ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Aegilops Tauschii ◽  
Wheat Breeding ◽  
Linkage Drag ◽  
Addition Line ◽  
Sequencing Data ◽  
Haynaldia Villosa ◽  
Breeding Programs ◽  
Homologous Genes ◽  
Flow Cytometric ◽  
Flow Cytometric Sorting

Abstract Background: Short arm of chromosome 6V (6VS) of Haynaldia villosa has been used in wheat breeding programs to introduce Pm21 resistance gene against powdery mildew and some other genes. Results: In this work, 6VS was isolated from a wheat ( Triticum aestivum ) - 6VS telosome addition line by flow cytometric sorting and sequenced by illumina technology. The assembly length was 230.39 Mb with contig N50 of 9,788 bp. The sequence annotation identified 3,276 high confidence genes supported by RNA sequencing data, representing about 2.3% of the chromosome arm sequence; repetitive elements accounted for 74.91% of the arm sequence. Sequences homologous to 6VS genes were identified on short arms of chromosomes 6A of T. urartu , 6D of Aegilops tauschii , 6A and 6B of T. dicoccoides , 6A, 6B and 6D of T. aestivum and 6H of Hordeum vulgare , revealing synteny relationships among these chromosome arms. Based on differences in intron size between the homologous genes on 6VS and 6AS/6BS/6DS of T. aestivum , 222 primer pairs were designed. Out of them, 120 amplified 6VS-specific products and are suitable as intron-target (IT) markers to trace the 6VS chromatin introduced into wheat. Conclusions: The results obtained and markers developed in this work will facilitate introduction of important genes to common wheat from its wild relative, while reducing the presence of unfavorable genes due to linkage drag.

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