Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato

2000 ◽  
Vol 101 (5-6) ◽  
pp. 918-924 ◽  
Author(s):  
C. C. Huang ◽  
Y.-Y. Cui ◽  
C. R. Weng ◽  
P. Zabel ◽  
P. Lindhout
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Chromosome 6 ◽  
Powdery Mildew Resistance Gene ◽  
Pcr Markers ◽  
Diagnostic Pcr ◽  
Mildew Resistance ◽  
Tomato Powdery Mildew

Identification of Powdery Mildew Resistance Gene in Pea Line X9002

2015 ◽  
Vol 41 (4) ◽  
pp. 515 ◽  
Author(s):  
Zhong-Yi WANG ◽  
Hai-Ning FU ◽  
Su-Li SUN ◽  
Can-Xin DUAN ◽  
Xiao-Fei WU ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

scholarly journals Using the 6RLKu Minichromosome of Rye (Secale cereale L.) to Create Wheat-Rye 6D/6RLKu Small Segment Translocation Lines with Powdery Mildew Resistance

2018 ◽  
Vol 19 (12) ◽  
pp. 3933 ◽  
Author(s):  
Haimei Du ◽  
Zongxiang Tang ◽  
Qiong Duan ◽  
Shuyao Tang ◽  
Shulan Fu
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Secale Cereale ◽  
Powdery Mildew Resistance ◽  
Translocation Chromosome ◽  
Translocation Line ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Small Segment ◽  
Secale Cereale L

Long arms of rye (Secale cereale L.) chromosome 6 (6RL) carry powdery mildew resistance genes. However, these sources of resistance have not yet been successfully used in commercial wheat cultivars. The development of small segment translocation chromosomes carrying resistance may result in lines carrying the 6R chromosome becoming more commercially acceptable. However, no wheat-rye 6RL small segment translocation line with powdery mildew resistance has been reported. In this study, a wheat-rye 6RLKu minichromosome addition line with powdery mildew resistance was identified, and this minichromosome was derived from the segment between L2.5 and L2.8 of the 6RLKu chromosome arm. Following irradiation, the 6RLKu minichromosome divided into two smaller segments, named 6RLKumi200 and 6RLKumi119, and these fragments participated in the formation of wheat-rye small segment translocation chromosomes 6DS/6RLKumi200 and 6DL/6RLKumi119, respectively. The powdery mildew resistance gene was found to be located on the 6RLKumi119 segment. Sixteen 6RLKumi119-specific markers were developed, and their products were cloned and sequenced. Nucleotide BLAST searches indicated that 14 of the 16 sequences had 91–100% similarity with nine scaffolds derived from 6R chromosome of S. cereale L. Lo7. The small segment translocation chromosome 6DL/6RLKumi119 makes the practical utilization in agriculture of powdery mildew resistance gene on 6RLKu more likely. The nine scaffolds are useful for further studying the structure and function of this small segment.

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