Development and characterization of Hordeum chilense chromosome-specific STS markers suitable for wheat introgression and marker-assisted selection

1999 ◽  
Vol 98 (5) ◽  
pp. 721-727 ◽  
Author(s):  
P. Hernández ◽  
M. Hemmat ◽  
N. F. Weeden ◽  
G. Dorado ◽  
A. Martín
Keyword(s):  
Marker Assisted Selection ◽  
Hordeum Chilense ◽  
Sts Markers

Identification of AFLP makers linked to non-seed shattering locus (sht1) in buckwheat and conversion to STS markers for marker-assisted selection

Genome ◽  
2004 ◽  
Vol 47 (3) ◽  
pp. 469-474 ◽  
Author(s):  
K Matsui ◽  
Y Kiryu ◽  
T Komatsuda ◽  
N Kurauchi ◽  
T Ohtani ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Bulked Segregant Analysis ◽  
Aflp Markers ◽  
Aflp Analysis ◽  
Common Buckwheat ◽  
Gene Encoding ◽  
Sts Markers ◽  
Self Compatibility ◽  
Almost All

Shattering habit in buckwheat has two forms: brittle pedicel and weak pedicel. Brittle pedicel is observed in wild buckwheat, but not in cultivated buckwheat. Brittle pedicel in buckwheat is produced by two complementary, dominant genes, Sht1 and Sht2. The sht1 locus is linked to the S locus; almost all common buckwheat cultivars possess the allele sht1. To detect molecular makers linked to the sht1 locus, we used amplified fragment-length polymorphism (AFLP) analysis in combination with bulked segregant analysis of segregating progeny of a cross between a non-brittle common buckwheat and a brittle self-compatible buckwheat line. We screened 312 primer combinations and constructed a linkage map around the sht1 locus by using 102 F2 plants. Five AFLP markers were linked to the sht1 locus. Two of these, e54m58/610 and e55m46/320, cosegregated with the sht1 locus without recombination. The two AFLP markers were converted to STS markers according to the sequence of the AFLPs. The STS markers are useful for marker-assisted selection of non-brittle pedicel plants and provides a stepping-stone for map-based cloning and characterization of the gene encoding non-brittle pedicel.Key words: Fagopyrum esculentum, brittle pedicel, self-compatibility, bulked segregant analysis.

Characterization of Overexpressed Bx7 Gene (Bx7OE) in Chinese and CIMMYT Wheats by STS Markers

2009 ◽  
Vol 35 (3) ◽  
pp. 403-411 ◽  
Author(s):  
Yan REN ◽  
Dan LIANG ◽  
Ping-Ping ZHANG ◽  
Zhong-Hu HE ◽  
Jing CHEN ◽  
...  
Keyword(s):  
Sts Markers

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

Euphytica ◽  
2014 ◽  
Vol 203 (1) ◽  
pp. 165-176 ◽  
Author(s):  
M. G. Mattera ◽  
C. M. Ávila ◽  
S. G. Atienza ◽  
A. Cabrera
Keyword(s):  
Molecular Characterization ◽  
Introgression Lines ◽  
Hordeum Chilense

Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and its Evaluation in Marker-Assisted Selection

Plant Disease ◽  
2021 ◽  
Author(s):  
Wenrui Wang ◽  
Huagang He ◽  
Huiming Gao ◽  
Hongxing Xu ◽  
Wenyue Song ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Wheat Breeding ◽  
Agronomic Performance ◽  
Breeding Line ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease seriously threatening yield and quality of common wheat (Triticum aestivum L., 2n=6x=42, AABBDD). Characterization of resistance genes against powdery mildew is useful in parental selection and for developing disease resistant cultivars. Chinese wheat breeding line KN0816 has superior agronomic performance and resistance to powdery mildew at all growth stages. Genetic analysis using populations of KN0816 crossed with different susceptible parents indicated that a single dominant gene, tentatively designated PmKN0816, conferred seedling resistance to different Bgt isolates. Using a bulked segregant analysis (BSA), PmKN0816 was mapped to the Pm6 interval on chromosome arm 2BL using polymorphic markers linked to the catalogued genes Pm6, Pm52, and Pm64, and flanked by markers CISSR02g-6 and CIT02g-2 both with genetic distances of 0.7 cM. Analysis of closely linked molecular markers indicated that the marker alleles of PmKN0816 differed from those of other powdery mildew resistance genes on 2BL, including Pm6, Pm33, Pm51, Pm64, and PmQ. Based on the genetic and physical locations and response pattern to different Bgt isolates, PmKN0816 is most likely a new powdery mildew resistance gene and confers effective resistance to all the 14 tested Bgt isolates. In view of the elite agronomic performance of KN0816 combined with the resistance, PmKN0816 is expected to become a valuable resistance gene in wheat breeding. To transfer PmKN0816 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers of PmKN0816 were evaluated and four of them (CIT02g-2, CISSR02g-6, CIT02g-10, and CIT02g-17) were confirmed to be applicable for MAS in different genetic backgrounds.

Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Southern catfish (Silurus meridionalis)

Aquaculture ◽  
2020 ◽  
Vol 517 ◽  
pp. 734783 ◽  
Author(s):  
Shuqing Zheng ◽  
Xiaoshuang Wang ◽  
Shuai Zhang ◽  
Juan Long ◽  
Wenjing Tao ◽  
...  
Keyword(s):  
Dna Markers ◽  
Marker Assisted Selection ◽  
Silurus Meridionalis

Cloning and molecular characterization of B-hordeins from Hordeum chilense (Roem. et Schult.)

2005 ◽  
Vol 111 (3) ◽  
pp. 551-560 ◽  
Author(s):  
F. Pistón ◽  
A. Martín ◽  
G. Dorado ◽  
F. Barro
Keyword(s):  
Molecular Characterization ◽  
Hordeum Chilense

Marker-assisted selection in Gossypium spp. for Meloidogyne incognita resistance and histopathological characterization of a near immune line

Euphytica ◽  
2020 ◽  
Vol 216 (2) ◽  
Author(s):  
C. M. L. Lopes ◽  
N. D. Suassuna ◽  
J. E. Cares ◽  
A. C. M. M. Gomes ◽  
F. J. Perina ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Marker Assisted Selection ◽  
Gossypium Spp

scholarly journals Development and Validation of High-Resolution Melting Markers Derived from Rysto STS Markers for High-Throughput Marker-Assisted Selection of Potato Carrying Rysto

2016 ◽  
Vol 106 (11) ◽  
pp. 1366-1375 ◽  
Author(s):  
Xianzhou Nie ◽  
Darcy Sutherland ◽  
Virginia Dickison ◽  
Mathuresh Singh ◽  
Agnes M. Murphy ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Marker Assisted Selection ◽  
High Resolution Melting ◽  
Chromosome Region ◽  
Sts Markers ◽  
Reverse Primer ◽  
Selection For ◽  
Development And Validation ◽  
Selection Of

Sequence analysis of the chromosome region harboring the sequence-tagged site (STS) markers YES3-3A and YES3-3B for Rysto, a gene responsible for extreme resistance to Potato virus Y (PVY) in potato, was performed in tetraploid potato ‘Barbara’ (Rrrr) and ‘AC Chaleur’ (rrrr) as well as their progeny selections. Three and two sequence variants were identified in Barbara resistant (R) selections and AC Chaleur susceptible (S) selections, respectively. Further analysis indicates that the variant with a 21-nucleotide (nt) deletion is likely the chromosome copy harboring the STS markers. Two primer pairs, one targeting the region containing a 20-nt deletion and the other targeting the region anchoring the YES3-3A reverse primer, were designed. As anticipated, pair one produced two visible fragments in Barbara-R bulk and one visible fragment in AC Chaleur-S bulk; pair two produced one visible fragment in all samples. When subjected to high-resolution melting (HRM) analysis, two distinct melting profiles for R and S samples were observed. Analysis of 147 progeny of Barbara × AC Chaleur revealed 72 and 75 progeny with R and S melting profiles, respectively, which was consistent with YES3-3A and YES3-3B assays and phenotyping analysis, thus demonstrating the potential of HRM profiles as novel molecular markers for Rysto. The efficacy of the newly developed HRM markers for high-throughput marker-assisted selection for Rysto-conferred resistance to PVY was validated further with three populations involving Barbara as the R parent.

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