Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth)

1998 ◽  
Vol 97 (8) ◽  
pp. 1235-1239 ◽  
Author(s):  
V. Ivandic ◽  
U. Walther ◽  
A. Graner
Keyword(s):  
Leaf Rust ◽  
Wild Barley ◽  
Molecular Mapping ◽  
Puccinia Hordei ◽  
Complete Resistance ◽  
New Gene

scholarly journals Identification and mapping of a leaf rust resistance gene in barley line Q21861

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 236-241 ◽  
Author(s):  
I. G. Borovkova ◽  
B. J. Steffenson ◽  
Y. Jin ◽  
A. Kilian ◽  
A. Kleinhofs ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Puccinia Hordei ◽  
Doubled Haploid Population ◽  
Leaf Rust Resistance Gene ◽  
Resistance Locus ◽  
Sequence Tagged Sites ◽  
Barley Line

Barley line Q21861 possesses an incompletely dominant gene (RphQ) for resistance to leaf rust caused by Puccinia hordei. To investigate the allelic and linkage relations between RphQ and other known Rph genes, F2 populations from crosses between Q21861 and donors of Rph1 to Rph14 (except for Rph8) were evaluated for leaf rust reaction at the seedling stage. Results indicate that RphQ is either allelic with or closely linked to the Rph2 locus. A doubled haploid population derived from a cross between Q21861 and SM89010 (a leaf rust susceptible line) was used for molecular mapping of the resistance locus. Bulked segregant analysis was used to identify markers linked to RphQ, using random amplified polymorphic DNAs (RAPDs), restriction fragment length polymorphisms (RFLPs), and sequence tagged sites (STSs). Of 600 decamer primers screened, amplified fragments generated by 9 primers were found to be linked to the RphQ locus; however, only 4 of them were within 10 cM of the target. The RphQ locus was mapped to the centromeric region of chromosome 7, with a linkage distance of 3.5 cM from the RFLP marker CDO749. Rrn2, an RFLP clone from the ribosomal RNA intergenic spacer region, was found to be very closely linked with RphQ, based on bulked segregant analysis. An STS marker, ITS1, derived from Rrn2, was also closely linked (1.6 cM) to RphQ.Key words: Hordeum vulgare, Puccinia hordei, allelism testing, linkage, molecular markers.

scholarly journals The barley leaf rust resistance gene Rph3 encodes a putative executor protein

2021 ◽  
Author(s):  
Hoan Dinh ◽  
Davinder Singh ◽  
Diana Cruz ◽  
Goetz Hensel ◽  
Martin Mascher ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Plant Disease Resistance ◽  
Wild Barley ◽  
Control Plant ◽  
Plant Diseases ◽  
Puccinia Hordei ◽  
Functional Markers ◽  
Leaf Rust Resistance Gene ◽  
Transcription Activator

Abstract Host resistance is considered the most effective means to control plant diseases; however, individually deployed resistance genes are often rapidly overcome by pathogen adaptation. Combining multiple effective resistance genes is the optimal approach to durable resistance, but the lack of functional markers for resistance genes has hampered implementation. Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major Resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3 gene, which originated from wild barley and was first introgressed into cultivated Egyptian germplasm, encodes a unique transmembrane resistance protein that differs from all known plant disease resistance proteins at the amino acid sequence level. Genetic profiles of diverse accessions indicated limited genetic diversity in Rph3 in domesticated germplasm, and higher diversity in wild barley from the Eastern Mediterranean region. Expression profiling using P. hordei isolates with contrasting pathogenicity for the Rph3 host locus showed that the Rph3 gene was expressed only in interactions with Rph3-avirulent isolates, a phenomenon also observed for transcription activator-like effector-dependent genes known as executors conferring resistance to Xanthomonas spp. Like the known transmembrane executors such as Bs3 and Xa7 heterologous expression of Rph3 in N. benthamiana induced a cell death response. Given that Rph3 shares several features with executor genes, it seems likely that P. hordei contains effectors similar to the transcription activator-like effectors that target host executor genes. The isolation of Rph3 highlights convergent evolutionary processes in diverse plant-pathogen interaction systems, where similar defence mechanisms evolved independently in monocots and dicots and provide evidence for executor genes in the Triticeae tribe.

Discovery and fine mapping of Rph28: a new gene conferring resistance to Puccinia hordei from wild barley

2021 ◽  
Author(s):  
M. Mehnaz ◽  
P. Dracatos ◽  
A. Pham ◽  
T. March ◽  
A. Maurer ◽  
...  
Keyword(s):  
Fine Mapping ◽  
Wild Barley ◽  
Puccinia Hordei ◽  
New Gene

scholarly journals BED‐domain‐containing NLR from wild barley confers resistance to leaf rust

2021 ◽  
Author(s):  
Chunhong Chen ◽  
Matthias Jost ◽  
Bethany Clark ◽  
Matthew Martin ◽  
Oadi Matny ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Wild Barley

Molecular Mapping of Leaf Rust Resistance Gene 5 in Barley

Crop Science ◽  
2003 ◽  
Vol 43 (1) ◽  
pp. 388 ◽  
Author(s):  
J. A. Mammadov ◽  
J. C. Zwonitzer ◽  
R. M. Biyashev ◽  
C. A. Griffey ◽  
Y. Jin ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Molecular mapping of a new gene for resistance to frogeye leaf spot of soya bean in 'Peking'

2001 ◽  
Vol 120 (1) ◽  
pp. 73-78 ◽  
Author(s):  
W. Yang ◽  
D. B. Weaver ◽  
B. L. Nielsen ◽  
J. Qiu
Keyword(s):  
Leaf Spot ◽  
Molecular Mapping ◽  
Soya Bean ◽  
Frogeye Leaf Spot ◽  
New Gene

Molecular mapping and markers for leaf rust resistance gene Lr24 in CIMMYT wheat line 19HRWSN-122

Euphytica ◽  
2015 ◽  
Vol 206 (1) ◽  
pp. 57-66
Author(s):  
Ai-yong Qi ◽  
Pei-pei Zhang ◽  
Xian-chun Xia ◽  
Zhong-hu He ◽  
Julio Huerta-Espino ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Wheat Line
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