Molecular markers linked to white rust resistance in mustard Brassica juncea

1998 ◽  
Vol 97 (5-6) ◽  
pp. 865-870 ◽  
Author(s):  
K. V. Prabhu ◽  
D. J. Somers ◽  
G. Rakow ◽  
R. K. Gugel
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Rust Resistance ◽  
White Rust

Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.)

2015 ◽  
Vol 95 (5) ◽  
pp. 939-945 ◽  
Author(s):  
Binay K. Singh ◽  
Divakar Nandan ◽  
Supriya Ambawat ◽  
Bhagirath Ram ◽  
Arun Kumar ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Rust Resistance ◽  
Indian Mustard ◽  
Resistance Locus ◽  
White Rust ◽  
Pcr Products ◽  
Percent Disease Index ◽  
Different Populations

Singh, B. K., Nandan, D., Supriya, A., Ram, B., Kumar, A., Singh, T., Meena, H. S., Kumar, V., Singh, V. V., Rai, P. K. and Singh, D. 2015. Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.). Can. J. Plant Sci. 95: 939–945. Successful application of molecular markers in marker-assisted pyramiding relies on effective determination of the target phenotype. In this respect, evaluation of the efficiency of markers for marker-assisted selection through cross-validation in different genetic backgrounds and in different populations is a crucial step. In the present study, the previously identified Arabidopsis-derived intron polymorphic (IP) markers At5g41560 and At2g36360, which were highly linked with AcB1-A4.1 and AcB1-A5.1, respectively, were validated in a set of 25 genotypes of Indian Mustard and in three different F2 populations. The relationships between the variation of PCR products of the two markers with the percent disease index (PDI) of the tested genotypes, and the co-segregation analysis of the markers with disease phenotype in F2 populations clearly indicated that At5g41560 and At2g36360 are genotype-nonspecific markers and are closely linked to white rust resistance loci AcB1-A4.1 and AcB1-A5.1, respectively. It also became evident from the present study that AcB1-A4.1 and an another white rust resistance locus Ac(2)t are likely the same gene locus.

Genetics of white rust resistance in Indian mustard (Brassica juncea L.) and its validation on using molecular markers

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
V. V. Singh ◽  
Monika Dubey ◽  
Neeraj Gurjar ◽  
Balbeer . ◽  
Priyamedha . ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Banding Pattern ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Indian Mustard ◽  
Single Dominant Gene ◽  
White Rust ◽  
Resistant Genotypes

White rust resistance loci (AcB1-A4.1 and AcB1-A5.1) associated with intron polymorphic (IP) markers i.e. At5g41560 and At2g36360, respectively, were used for validation of P1, P2, F1, F2, BC1F1 and BC2F1 generations. The donor parents namely, Bio-YSR and BEC-144 produced desired banding pattern of 430 and 750 bp while recipients viz., NRCHB 101 and DRMR-150-35 exhibited different pattern from donors confirming white rust resistance loci 4.1 and 5.1 with marker At5g41560 and At2g36360, respectively. Confirmation of these set of two IP markers in the parents and F1s lead us to further screening of selected F2, BC1F1 and BC2F1 populations. Available data on white rust reaction in different generations under study revealed that single dominant gene is responsible for white rust resistance. Potential of molecular markers in developing white rust resistant genotypes is proved under present study.

scholarly journals Deciphering the Molecular Architecture of a Candidate R-gene (BjuWRR1) Product Mediating White Rust Resistance in Brassica juncea

2021 ◽  
Vol 12 (5) ◽  
pp. 393-401
Author(s):  
Chinmaya Kumar Das ◽  
◽  
Umasankar Nayak ◽  
Preetinanda Pati ◽  
Mihir Ranjan Mohanty ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Molecular Interaction ◽  
Rust Resistance ◽  
Hydrophobic Interactions ◽  
Coiled Coil ◽  
R Gene ◽  
Molecular Architecture ◽  
Three Dimensional Model ◽  
White Rust ◽  
Rust Pathogen

In this investigation, a three-dimensional model of a R-gene encoded product BjuWRR1 which is known to play a role in white rust resistance in Brassica juncea was developed to synthesize innovative ways for evolving white rust resistant cultivars. The model was built from the amino acid sequence of BjuWRR1 using structural template information of a disease resistance protein (RPP13-like protein 4 of Arabidopsis thaliana) with the help of homology-based modelling approach. Built models were validated for their stereochemical parameters and structural descriptors using Ramachandran plot analysis, protein structure analysis and ERRAT analysis. Structural analysis of BjuWRR1 model revealed that it is composed of three distinct domains namely a coiled-coil domain, a central NB-ARC nucleotide binding domain and a hypervariable leucine-rich repeat domain. Further, canonical conserved motifs such as P-loop, Kinase2-motif and HD-motif were found in the NB-ARC domain. The built model would help in understanding the molecular basis of plant-immunity against white rust pathogen by understanding the significance of inter-domain interactions in BuWRR1 in triggering the activation of downstream defense response against the white rust pathogen by promoting oligomerization of coiled-coil domains through stabilized hydrophobic interactions and interaction with NB-ARC domain. Presence of patches of charged residues in each domain of BjuWRR1 indicated their possible role in intra-molecular interaction with other domains. Therefore, this model can help in designing functional genomic studies to understand the role of intra-molecular interaction in BjuWRR1 to mediate resistance against white rust pathogen.

Identification of RFLP markers linked to the white rust resistance gene (Acr) in mustard (Brassica juncea (L.) Czern. and Coss.)

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 626-628 ◽  
Author(s):  
W Y Cheung ◽  
R K Gugel ◽  
B S Landry
Keyword(s):  
Brassica Juncea ◽  
Rust Resistance ◽  
Single Gene ◽  
Rapid Identification ◽  
Rflp Markers ◽  
Rust Disease ◽  
Albugo Candida ◽  
White Rust ◽  
Rflp Map ◽  
Race 2

White rust and staghead, caused by Albugo candida, is an economically important disease of Brassica juncea and Brassica rapa crops in western Canada. The identification of genes for white rust resistance in these crops and the development of molecular markers for these genes will allow the rapid identification of resistant germplasm and should accelerate the development of white rust resistant cultivars. In this study, 119 F1-derived doubled-haploid progeny lines of a cross between white rust susceptible (J90-4317) and white rust resistant (J90-2733) B. juncea lines were evaluated for resistance to A. candida race 2. A single gene (Acr) responsible for conferring resistance to this pathogen was mapped on a densely populated B. juncea RFLP map developed earlier. A cosegregating RFLP marker (X140a) and two other closely linked RFLP markers (X42 and X83) were identified; the latter two markers were 2.3 and 4 cM from the Acr locus, respectively. These markers may be useful for marker-assisted selection and map-based cloning of this gene.Key words: Brassica juncea, mustard, Albugo candida, white rust, disease resistance, RFLP.

Inheritance of White Rust Resistance in Brassica juncea

1986 ◽  
Vol 97 (1) ◽  
pp. 75-77 ◽  
Author(s):  
S. K. uhukral ◽  
Hari Singh
Keyword(s):  
Brassica Juncea ◽  
Rust Resistance ◽  
White Rust
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