Sequence-tagged-site (STS) markers of arbitrary genes: the utility of black spruce-derived STS primers in other conifers

1998 ◽  
Vol 97 (5-6) ◽  
pp. 735-743 ◽  
Author(s):  
D. J. Perry ◽  
J. Bousquet
Keyword(s):  
Black Spruce ◽  
Sequence Tagged Site ◽  
Sts Markers

Genetic diversity and mating system of post-fire and post-harvest black spruce: an investigation using codominant sequence-tagged-site (STS) markers

2001 ◽  
Vol 31 (1) ◽  
pp. 32-40 ◽  
Author(s):  
Daniel J Perry ◽  
Jean Bousquet
Keyword(s):  
Genetic Diversity ◽  
Mating System ◽  
Negative Impact ◽  
Black Spruce ◽  
Sequence Tagged Site ◽  
Sts Markers ◽  
Advance Regeneration ◽  
Spruce Stands ◽  
Fixation Indices ◽  
Fire Origin

cDNA-based sequence-tagged-site (STS) markers were used to examine the genetic composition of three mature, layer-origin populations of black spruce (Picea mariana (Mill.) BSP), which were the result of logging operations in the first half of the 20th century, and compare them with four mature, seedling-origin populations that regenerated naturally following fire. The amount of STS-marker variation revealed in these populations was very similar to that previously observed in a rangewide panel of black spruce trees. There was little differentiation among populations, and no significant differences in heterozygosities, numbers of alleles, or fixation indices were evident between layer-origin and fire-origin stands. Likewise, when mating-system parameters were estimated in one population of each of these two types, no significant differences were found; outcrossing was essentially complete with no evidence of mating among relatives. The estimated correlation of paternity within progeny arrays was about 17 and 13% in the fire-origin and layer-origin stands, respectively, but again the observed difference was not statistically significant. At least at the current scale of sampling, silvicultural practices that result in stand replacement by layer-origin advance regeneration appear not to have had negative impact upon the genetic diversity or level of inbreeding in second-growth black spruce stands.

scholarly journals Sequence-Tagged-Site (STS) Markers of Arbitrary Genes: Development, Characterization and Analysis of Linkage in Black Spruce

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 1089-1098
Author(s):  
Daniel J Perry ◽  
Jean Bousquet
Keyword(s):  
Black Spruce ◽  
Pcr Primers ◽  
Mendelian Inheritance ◽  
Length Variation ◽  
Direct Repeats ◽  
Noncoding Regions ◽  
Sequence Tagged Site ◽  
Sts Markers ◽  
Length Polymorphisms ◽  
Allelic Segregation

Abstract Sequence-tagged-site (STS) markers of arbitrary genes were investigated in black spruce [Picea mariana (Mill.) B.S.P.]. Thirty-nine pairs of PCR primers were used to screen diverse panels of haploid and diploid DNAs for variation that could be detected by standard agarose gel electrophoresis without further manipulation of amplification products. Codominant length polymorphisms were revealed at 15 loci. Three of these loci also had null amplification alleles as did 3 other loci that had no apparent product-length variation. Dominant length polymorphisms were observed at 2 other loci. Alleles of codominant markers differed in size by as little as 1 bp to as much as an estimated 175 bp with nearly all insertions/deletions found in noncoding regions. Polymorphisms at 3 loci involved large (33 bp to at least 114 bp) direct repeats and similar repeats were found in 7 of 51 cDNAs sequenced. Allelic segregation was in accordance with Mendelian inheritance and linkage was detected for 5 of 63 pairwise combinations of loci tested. Codominant STS markers of 12 loci revealed an average heterozygosity of 0.26 and an average of 2.8 alleles in a range-wide sample of 22 trees.

scholarly journals Yeast artificil chromosome (YAC) clones and sequence tagged site (STS) markers anchored at human chromosome 21

1991 ◽  
Vol 36 (4) ◽  
pp. 331-334 ◽  
Author(s):  
Toshihiko Eki ◽  
Kazushige Yokoyama ◽  
Hiroyuki Tashiro ◽  
Kazuo Ozawa ◽  
Yasufumi Murakami ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 21 ◽  
Sequence Tagged Site ◽  
Human Chromosome 21 ◽  
Sts Markers

scholarly journals Sequence Tagged Site Markers to Rsp1, Rsp2, and Rsp3 Genes for Resistance to Septoria Speckled Leaf Blotch in Barley

2007 ◽  
Vol 97 (2) ◽  
pp. 162-169 ◽  
Author(s):  
S. H. Lee ◽  
S. M. Neate
Keyword(s):  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Gene Pyramiding ◽  
Sts Marker ◽  
Leaf Blotch ◽  
Repulsion Phase ◽  
Sequence Tagged Site ◽  
Sts Markers ◽  
Site Markers ◽  
Barley Germplasm

Five random amplified polymorphic DNA markers, two in coupling (OPAH5545C, and OPBA12314C) and three in repulsion phase (UBC285158R, OPC2441R, and OPB17451R), closely linked to Rsp genes conferring resistance to Septoria speckled leaf blotch (SSLB), were identified using bulked segregant analysis in three F2 populations, each containing a Rsp gene. These markers were converted into the sequence tagged site (STS) markers SUBC285, SOPC2, SOPAH5, and SOPBA12. Another STS marker (MWG938) linked to Rsp2 in coupling phase was also identified in an F2 population from the cross Robust/CIho 4780. The STS markers were tested on a set of 42 resistant and susceptible barley germplasm lines and 98 landraces. The expected sizes of marker fragments associated with each allele at Rsp loci were present in resistant or susceptible accessions. Efficiency of marker-assisted selection (MAS) for Rsp1, Rsp2, and Rsp3 using STS markers were evaluated in three F2–3 populations in the greenhouse and the field. Results of testing F2–3 progeny demonstrated that the accuracy of MAS was, with one exception, greater than 97% in the greenhouse and in two field locations (90% in the Osnabrock, ND trial for Rsp2). The STS markers closely linked to Rsp genes also identified the SSLB resistance corresponding to Rsp1, Rsp2, or Rsp3 in gene pyramiding F2 populations. The STS markers tightly linked to Rsp genes may be useful for M and for pyramiding with other genes in barley breeding for SSLB resistance.

scholarly journals Sequence-tagged-site (STS) markers of arbitrary genes: the amount and nature of variation revealed in Norway spruce

Heredity ◽  
1999 ◽  
Vol 83 (3) ◽  
pp. 239-248 ◽  
Author(s):  
Daniel J Perry ◽  
Nathalie Isabel ◽  
Jean Bousquet
Keyword(s):  
Norway Spruce ◽  
Sequence Tagged Site ◽  
Sts Markers

Antitranspirant action of abscisic acid and ten synthetic analogs in black spruce

1990 ◽  
Vol 80 (3) ◽  
pp. 365-370 ◽  
Author(s):  
Terence J. Blake ◽  
Weixing Tan ◽  
Suzanne R. Abrams
Keyword(s):  
Abscisic Acid ◽  
Black Spruce ◽  
Synthetic Analogs

Fertilization of black spruce or poor site peatland in Minnesota.

1981 ◽  
Author(s):  
David H. Alban ◽  
Richard F. Watt
Keyword(s):  
Black Spruce ◽  
Poor Site

Characterization of Overexpressed Bx7 Gene (Bx7OE) in Chinese and CIMMYT Wheats by STS Markers

2009 ◽  
Vol 35 (3) ◽  
pp. 403-411 ◽  
Author(s):  
Yan REN ◽  
Dan LIANG ◽  
Ping-Ping ZHANG ◽  
Zhong-Hu HE ◽  
Jing CHEN ◽  
...  
Keyword(s):  
Sts Markers
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