A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines

1998 ◽  
Vol 97 (1-2) ◽  
pp. 83-89 ◽  
Author(s):  
I. Eujayl ◽  
M. Baum ◽  
W. Powell ◽  
W. Erskine ◽  
E. Pehu
2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Sungwoo Lee ◽  
Keith R. Freewalt ◽  
Leah K. McHale ◽  
Qijian Song ◽  
Tae-Hwan Jun ◽  
...  

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 425-438 ◽  
Author(s):  
Li-Jing Chen ◽  
Hong-Mei Li ◽  
Shao-Kun Sun ◽  
Muhammad Irfan ◽  
Jing-Wei Lin ◽  
...  

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium.


2004 ◽  
Vol 129 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Renbing Zhang ◽  
Yong Xu ◽  
Ke Yi ◽  
Haiying Zhang ◽  
Ligong Liu ◽  
...  

A genetic linkage map was constructed for watermelon using 117 recombinant inbred lines (RILs) (F2S7) descended from a cross between the high quality inbred line 97103 [Citrullus lanatus var. lanatus (Thunb.) Matsum. & Nakai] and the Fusarium wilt (races 0, 1, and 2) resistant U.S. Plant Introduction (PI) 296341 (C. lanatus var. citroides). The linkage map contains 87 randomly amplified polymorphic DNA (RAPD) markers, 13 inter simple sequence repeat (ISSR) markers, and four sequenced characterized amplified region (SCAR) markers. The map consists of 15 linkage groups. Among them are a large linkage group of 31 markers covering a mapping distance of 277.5 cM, six groups each with 4 to 12 markers covering a mapping distance of 51.7 to 172.2 cM, and eight small groups each with 2-5 markers covering a mapping distance of 7.9 to 46.4 cM. The map covers a total distance of 1027.5 cM with an average distance of 11.7 cM between two markers. The map is useful for the further development of quantitative trait loci (QTLs) affecting fruit qualities and for identification of genes conferring resistance to Fusarium wilt (races 0, 1 and 2). The present map can be used for further construction of a reference linkage map for watermelon based on an immortalized mapping population with progenies homozygous for most gene loci.


Genome ◽  
2002 ◽  
Vol 45 (1) ◽  
pp. 175-188 ◽  
Author(s):  
J T Ouédraogo ◽  
B S Gowda ◽  
M Jean ◽  
T J Close ◽  
J D Ehlers ◽  
...  

An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population of 94 recombinant inbred lines (RILs) derived from the cross between 'IT84S-2049' and '524B'. A set of 242 molecular markers, mostly amplified fragment length polymorphism (AFLP), linked to 17 biological resistance traits, resistance genes, and resistance gene analogs (RGAs) were scored for segregation within the parental and recombinant inbred lines. These data were used in conjunction with the 181 random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), AFLP, and biochemical markers previously mapped to construct an integrated linkage map for cowpea. The new genetic map of cowpea consists of 11 linkage groups (LGs) spanning a total of 2670 cM, with an average distance of 6.43 cM between markers. Astonishingly, a large, contiguous portion of LG1 that had been undetected in previous mapping work was discovered. This region, spanning about 580 cM, is composed entirely of AFLP markers (54 in total). In addition to the construction of a new map, molecular markers associated with various biological resistance and (or) tolerance traits, resistance genes, and RGAs were also placed on the map, including markers for resistance to Striga gesnerioides races 1 and 3, CPMV, CPSMV, B1CMV, SBMV, Fusarium wilt, and root-knot nematodes. These markers will be useful for the development of tools for marker-assisted selection in cowpea breeding, as well as for subsequent map-based cloning of the various resistance genes.Key words: Cowpea, AFLP, RAPD, resistance gene analogs, genetic linkage map.


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 453D-453
Author(s):  
Norman Weeden ◽  
Gail Timmerman-Vaughan

A linkage map for a set of 51 F2-derived recombinant inbred lines has been constructed from the segregation data of ≈850 morphological, isozyme, RFLP, STS, RAPD, and AFLP markers. The final map consists of seven clear linkage groups with a total length of nearly 900 cM. The wide variety of loci placed on this map permits its comparison with partial maps that have been developed in other programs. For the most part, the arrangement of loci agrees with that in previous maps, and no evidence for translocation heterozygosity in this cross is apparent. Although some clustering of markers is observed, for the most part the markers are well-distributed, and few gaps greater than 5 cM are found in the coverage. The availability of this first “complete” and highly saturated map for pea should permit more efficient comparison of the partial maps that have been generated in a number of different crosses, as well as provide a firm basis for future mapping and molecular studies in this species.


2005 ◽  
Vol 110 (4) ◽  
pp. 669-677 ◽  
Author(s):  
A. Hamwieh ◽  
S. M. Udupa ◽  
W. Choumane ◽  
A. Sarker ◽  
F. Dreyer ◽  
...  

2009 ◽  
Vol 18 (5) ◽  
pp. 825-835 ◽  
Author(s):  
Wei-Ji Wang ◽  
Han-Ping Wang ◽  
Hong Yao ◽  
Geoff K. Wallat ◽  
Laura G. Tiu ◽  
...  

2010 ◽  
pp. 141-148 ◽  
Author(s):  
Y.S. Guo ◽  
C.J. Liu ◽  
Y.H. Zhao ◽  
J.H. Xie ◽  
P.R. Ren ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document