Molecular characterization of the genome composition of partial amphiploids derived from Triticum aestivum×Thinopyrum ponticum and T. aestivum×Th. intermedium as sources of resistance to wheat streak mosaic virus and its vector, Aceria tosichella

1998 ◽  
Vol 97 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Q. Chen ◽  
R. L. Conner ◽  
F. Ahmad ◽  
A. Laroche ◽  
G. Fedak ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Mosaic Virus ◽  
Molecular Characterization ◽  
Wheat Streak Mosaic Virus ◽  
Genome Composition ◽  
Sources Of Resistance ◽  
Thinopyrum Ponticum ◽  
Aceria Tosichella ◽  
Partial Amphiploids

scholarly journals Development of Serological Procedures for Rapid and Reliable Detection of Wheat Streak Mosaic Virus in a Single Wheat Curl Mite

Plant Disease ◽  
1997 ◽  
Vol 81 (3) ◽  
pp. 250-253 ◽  
Author(s):  
T. Mahmood ◽  
G. L. Hein ◽  
R. C. French
Keyword(s):  
Triticum Aestivum ◽  
Mosaic Virus ◽  
Wheat Streak Mosaic Virus ◽  
Immunofluorescent Microscopy ◽  
Wheat Curl Mite ◽  
Aceria Tosichella ◽  
Reliable Detection

Wheat streak mosaic virus (WSMV) is transmitted by the wheat curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous WCM. With dot-immunobinding assay, WSMV was detected in WCM fed on WSMV-infected wheat (Triticum aestivum) but not in WCM maintained on healthy plants. Both immunofluorescent and dot-immunobinding assays were sufficiently sensitive to detect WSMV in individual WCM, providing a means to determine the percentage of viruliferous WCM in field collections.

Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum × Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPD

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1062-1071 ◽  
Author(s):  
Xueyong Zhang ◽  
Yushen Dong ◽  
Richard R.-C. Wang
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
Thinopyrum Ponticum ◽  
Genome Recombination ◽  
Partial Amphiploid ◽  
Thinopyrum Bessarabicum ◽  
Thinopyrum Elongatum ◽  
Partial Amphiploids

Genomic in situ hybridization (GISH) and Southern hybridization of genome-specific RAPD markers were used to demonstrate that the E genome (including Ee and Eb from Thinopyrum elongatum and Thinopyrum bessarabicum, respectively) and the St genome (from Pseudoroegneria species) were the two basic genomes in Thinopyrum ponticum. GISH also revealed that the centromeric region may be the critical area that discriminates the St genome from the E genome in Th. ponticum. Of the seven partial amphiploids isolated from backcrossed progenies of Triticum aestivum × Thinopyrum ponticum hybrids, two (lines 693 and 7631) have eight pairs of chromosomes from the Ee and (or) Eb genomes. Four partial amphiploids (lines 784, 68, 7430, and 40767-1) have an incomplete St genome, i.e., six pairs of chromosomes of St and one pair of chromosomes from Ee or Eb. In a heptaploid individual of the partial amphiploid 40767-2, there were four pairs of St chromosomes, one pair of St/1B Robertsonian translocation chromosomes, one pair of St/E translocation chromosomes, and one pair of Ee or Eb chromosomes. The isoelectric focusing of Est-5, Est-4, β-Amy-1, α-Amy-1, and α-Amy-2 and the RAPD data generated with 24 decamer primers on five partial amphiploids (lines 784, 693, 7631, 68, and 7430) indicated that lines 693 and 7631 had identical genomes from Th. ponticum. The partial amphiploid 784 probably had a set of chromosomes completely different from those of 693 and 7631. These results indicate that genome recombination usually occurred during the formation of new polyploid lines. Key words : Thinopyrum ponticum, wheat, partial amphiploid, GISH, isozyme, RAPD.

Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica)

2009 ◽  
Vol 37 (4) ◽  
pp. 375-379 ◽  
Author(s):  
Tombisana Thokchom ◽  
Tanuja Rana ◽  
Vipin Hallan ◽  
Raja Ram ◽  
Aijaz A. Zaidi
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Malus Domestica ◽  
Indian Strain

scholarly journals Detection and molecular characterization of Pepper mild mottle virus in Serbia

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 651-663 ◽  
Author(s):  
Dragana Milosevic ◽  
Ivana Stankovic ◽  
Aleksandra Bulajic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Great Influence ◽  
Alfalfa Mosaic Virus ◽  
Resistance Breeding ◽  
Elisa Test ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus ◽  
Das Elisa

During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P1,2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.

Molecular Characterization of the 3'-Terminal Region of Turnip mosaic virus Isolates from Eastern China

2007 ◽  
Vol 155 (6) ◽  
pp. 333-341 ◽  
Author(s):  
Y.-P. Tian ◽  
X.-P. Zhu ◽  
J.-L. Liu ◽  
X.-Q. Yu ◽  
J. Du ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Eastern China ◽  
Turnip Mosaic Virus ◽  
Terminal Region ◽  
Virus Isolates
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