A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene

1998 ◽  
Vol 96 (2) ◽  
pp. 196-202 ◽  
Author(s):  
D. Danesh ◽  
S. Peñuela ◽  
J. Mudge ◽  
R. L. Denny ◽  
H. Nordstrom ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Resistance Gene ◽  
Bacterial Artificial Chromosome Library ◽  
Artificial Chromosome ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Nematode Resistance Gene

Physical mapping of resistant and susceptible soybean genomes near the soybean cyst nematode resistance gene Rhg4

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1057-1064 ◽  
Author(s):  
K S Lewers ◽  
S D Nilmalgoda ◽  
A L Warner ◽  
H T Knap ◽  
B F Matthews
Keyword(s):  
Resistance Gene ◽  
Soybean Cyst Nematode ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Nematode Resistance ◽  
Physical Distance ◽  
Cyst Nematode ◽  
Nematode Resistance Gene ◽  
Glycine Max L ◽  
Soybean Cyst Nematode Resistance

The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is the foremost pest of soybean (Glycine max L. Merr.). The rhg1 allele on linkage group (LG) G and the Rhg4 allele on LG A2 are important in conditioning resistance. Markers closely linked to the Rhg4 locus were used previously to screen a library of bacterial artificial chromosome (BAC) clones from susceptible 'Williams 82' and identified a single 150-kb BAC, Gm_ISb001_056_G02 (56G2). End-sequenced subclones positioned onto a restriction map provided landmarks for identifying the corresponding region from a BAC library from accession PI 437654 with broad resistance to SCN. Seventy-three PI 437654 BACs were assigned to contigs based upon HindIII restriction fragment profiles. Four contigs represented the PI 437654 counterpart of the 'Williams 82' BAC, with PCR assays connecting these contigs. Some of the markers on the PI 437654 contigs are separated by a greater physical distance than in the 'Williams 82' BAC and some primers amplify bands from BACs in the mid-portion of the connected PI 437654 BAC contigs that are not amplified from the 'Williams 82' BAC. These observations suggest that there is an insertion in the PI 437654 genome relative to the 'Williams 82' genome in the Rhg4 region.Key words: BAC, deletion, insertion, resistance gene, soybean cyst nematode.

Physical mapping of resistant and susceptible soybean genomes near the soybean cyst nematode resistance gene Rhg4

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1057-1064 ◽  
Author(s):  
K.S. Lewers ◽  
S.D. Nilmalgoda ◽  
A.L. Warner ◽  
H.T. Knap ◽  
B.F. Matthews
Keyword(s):  
Resistance Gene ◽  
Physical Mapping ◽  
Soybean Cyst Nematode ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Nematode Resistance Gene ◽  
Soybean Cyst Nematode Resistance

Cloning and sequence analysis of the leucine-rich repeats region of cereal cyst nematode resistance gene

2006 ◽  
Vol 3 (3) ◽  
pp. 231-235
Author(s):  
Zhai Xu-Guang ◽  
Liu Yi ◽  
Wu Fang ◽  
Deng Guang-Bing ◽  
Pan Zhi-Fen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Resistance Gene ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Coding Region ◽  
Sequence Identity ◽  
Nematode Resistance Gene

AbstractAccording to the sequence of Rccn4, which is highly similar to the nucleotide-binding site (NBS) coding region of the cereal cyst nematode resistance gene, Cre3, three 3′ nested primers were designed to amplify its 3′ flanking region through single oligonucleotide nested polymerase chain reaction (SON-PCR). One 1264 bp band, Rccn-L, was amplified from E-10, a wheat–Aegilops variabilis translocation line containing the cereal cyst nematode resistance gene from Ae. variabilis. Sequence analysis showed that Rccn-L possesses the 3′ flanking sequence of Rccn4 and contains a 55 bp-sized consensus sequence with Rccn4. The coding region was 1026 bp, consisting of an incomplete open reading frame, a terminator codon and no initiation codon and intron; it encoded a peptide of 342 amino acid residues and shared 86% nucleotide sequence identity with Cre3. The peptide had a conserved leucine-rich repeat (LRR) domain, containing the imperfect repeats, XXLXXLXXL, comprising 17% leucine residues, and shares, respectively, 89% nucleotide sequence and 78% amino acid sequence identity with the LRR sequence of the Cre3 locus. In the present study, SON-PCR was used successfully, for the first time, in plant genome research and proved to be a valuable method in plant gene cloning. The acquirement of Rccn-L established the foundation for obtaining the complete Rccn gene and further structural and functional investigations.

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