Differentiation of phytoplasmas associated with sugarcane and gramineous weed white leaf disease and sugarcane grassy shoot disease by RFLP and sequencing

1997 ◽  
Vol 95 (4) ◽  
pp. 660-663 ◽  
Author(s):  
P. Wongkaew ◽  
Y. Hanboonsong ◽  
P. Sirithorn ◽  
C. Choosai ◽  
S. Boonkrong ◽  
...  
Keyword(s):  
Leaf Disease ◽  
White Leaf ◽  
Sugarcane Grassy Shoot Disease ◽  
White Leaf Disease ◽  
Grassy Shoot Disease

scholarly journals Candidates for Symbiotic Control of Sugarcane White Leaf Disease

2012 ◽  
Vol 78 (19) ◽  
pp. 6804-6811 ◽  
Author(s):  
Jureemart Wangkeeree ◽  
Thomas A. Miller ◽  
Yupa Hanboonsong
Keyword(s):  
Developmental Stages ◽  
Cloning And Sequencing ◽  
Content Type ◽  
Leaf Disease ◽  
White Leaf ◽  
Symbiotic Control ◽  
Sugarcane White Leaf ◽  
White Leaf Disease ◽  
Bacterial Genes

ABSTRACTThe leafhopperMatsumuratettix hiroglyphicus(Matsumura) is the most important vector of a phytoplasma pathogen causing sugarcane white leaf (SCWL) disease. The purpose of this study was to evaluate candidate bacterial symbionts for possible use as vehicles in the control of the disease. 16S rRNA bacterial genes were amplified from whole bodies ofM. hiroglyphicusleafhoppers and analyzed by cloning and sequencing. Two dominant groups were found: one belonged to theBetaproteobacteriathat did not closely match any sequences in the database and was named bacterium associated withM. hiroglyphicus(BAMH). Another one found to be abundant in this leafhopper is “CandidatusSulcia muelleri” in the orderBacteroidetes, which was previously reported in the insect members of the Auchenorrhyncha. MostM. hiroglyphicusleafhoppers carry both BAMH and “Ca. Sulcia muelleri.” Fluorescentin situhybridization showed that BAMH and “Ca. Sulcia muelleri” colocalized in the same bacteriomes. BAMH was present in the midgut and ovaries of the leafhopper and was found in all developmental stages, including eggs, nymphs, and adults. Because BAMH appears to be specific for the SCWL vector, we evaluated it as a candidate for symbiotic control of sugarcane white leaf disease.

scholarly journals First report of a 'Candidatus Phytoplasma trifolii'-related strain (16SrVI-A) associated with white leaf disease in Spirea japonica

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Eray Şimşek ◽  
Hümeyra Ayvacı ◽  
Havva Akkurak ◽  
Murat Dikilitas ◽  
Mehmet Ertuğrul Güldür
Keyword(s):  
Related Strain ◽  
First Report ◽  
Leaf Disease ◽  
White Leaf ◽  
White Leaf Disease

scholarly journals Discovery of potential protein biomarkers associated with sugarcane white leaf disease susceptibility using a comparative proteomic approach

PeerJ ◽  
2022 ◽  
Vol 10 ◽  
pp. e12740
Author(s):  
Kantinan Leetanasaksakul ◽  
Sittiruk Roytrakul ◽  
Narumon Phaonakrop ◽  
Suthathip Kittisenachai ◽  
Siriwan Thaisakun ◽  
...  
Keyword(s):  
Metal Ion ◽  
Defense Mechanism ◽  
Potential Candidate ◽  
Protein Biomarkers ◽  
Sugarcane Varieties ◽  
Leaf Disease ◽  
Proteomic Approach ◽  
White Leaf ◽  
Sugarcane White Leaf ◽  
White Leaf Disease

Sugarcane white leaf disease (SCWLD) is caused by phytoplasma, a serious sugarcane phytoplasma pathogen, which causes significant decreases in crop yield and sugar quality. The identification of proteins involved in the defense mechanism against SCWLD phytoplasma may help towards the development of varieties resistant to SCWLD. We investigated the proteomes of four sugarcane varieties with different levels of susceptibility to SCWLD phytoplasma infection, namely K88-92 and K95-84 (high), KK3 (moderate), and UT1 (low) by quantitative label-free nano-liquid chromatography-tandem mass spectrometry (nano LC-MS/MS). A total of 248 proteins were identified and compared among the four sugarcane varieties. Two potential candidate protein biomarkers for reduced susceptibility to SCWLD phytoplasma were identified as proteins detected only in UT1. The functions of these proteins are associated with protein folding, metal ion binding, and oxidoreductase. The candidate biomarkers could be useful for further study of the sugarcane defense mechanism against SCWLD phytoplasma, and in molecular and conventional breeding strategies for variety improvement.

scholarly journals Wolbachia (Rickettsiales: Alphaproteobacteria) Infection in the Leafhopper Vector of Sugarcane White Leaf Disease

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Jureemart Wangkeeree ◽  
Panida Tewaruxsa ◽  
Jariya Roddee ◽  
Yupa Hanboonsong
Keyword(s):  
Biological Control Agent ◽  
Insect Pest ◽  
Geographic Location ◽  
Control Agent ◽  
Phylogenic Tree ◽  
Gene Markers ◽  
Leaf Disease ◽  
White Leaf ◽  
White Leaf Disease ◽  
Wsp Gene

Abstract Wolbachia is a maternally inherited bacterium ubiquitous in insects that has attracted interest as a prospective insect pest-control agent. Here, we detected and characterized Wolbachia in the leafhoppers Matsumuratettix hiroglyphicus (Matsumura) (Cicadellidae: Hemiptera) and Yamatotettix flavovittatus Matsumura (Cicadellidae: Hemiptera), insect vectors of the phytoplasma that cause white leaf disease in sugarcane. The 16S rRNA and wsp gene markers revealed that Wolbachia was not present in the M. hiroglyphicus but naturally occurs in Y. flavovittatus. Additionally, the infection rates in adult leafhoppers ranged from 0 to 100% depending on geographic location. Moreover, Wolbachia was detected in the eggs and first- to fifth-instar nymphs of Y. flavovittatus. A phylogenic tree of Wolbachia indicated that it resided in the monophyletic supergroup B clade and clustered in the Ori subgroup. Furthermore, fluorescence in situ hybridization revealed that Wolbachia localized to the egg apices, randomly distributed in the egg cytoplasm, and was concentrated in the nymph and adult bacteriomes, as well as occasional detection in the thorax and abdomen. To the best of our knowledge, the present study is the first to demonstrate the prevalence of Wolbachia in the leafhopper Y. flavovittatus. The obtained results would provide useful information for the future development of Wolbachia as a biological control agent for the leafhopper vectors.

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