High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato

1997 ◽  
Vol 95 (1-2) ◽  
pp. 153-162 ◽  
Author(s):  
A. Bendahmane ◽  
K. Kanyuka ◽  
D. C. Baulcombe
Keyword(s):  
High Resolution ◽  
Potato Virus ◽  
Physical Mapping ◽  
Potato Virus X ◽  
Extreme Resistance ◽  
Tetraploid Potato

scholarly journals High Resolution DNA Melting Assays for Detection of Rx1 and Rx2 for High-Throughput Marker-Assisted Selection for Extreme Resistance to Potato virus X in Tetraploid Potato

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 382-390 ◽  
Author(s):  
Xianzhou Nie ◽  
Virginia L. Dickison ◽  
Sydney Brooks ◽  
Bihua Nie ◽  
Mathuresh Singh ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Potato Virus ◽  
Gel Electrophoresis ◽  
Potato Virus X ◽  
Dna Melting ◽  
Extreme Resistance ◽  
Reliable Detection ◽  
Selection For ◽  
Selection Of

Assessment of the existing PCR-gel electrophoresis-based methods for detection of Rx1 and Rx2, the genes that independently control extreme resistance (ER) to Potato virus X (PVX), indicated that the 5Rx1F/5Rx1R primer pair led to reliable detection of Rx1, whereas the 106Rx2F/106Rx2R primer pair detected Rx2 despite some nonspecific reactions in potato clones/cultivars without Rx2. However, the methodology is time consuming and does not differentiate the absence of Rx1/Rx2 from a failed PCR reaction. A newly designed primer pair that targets Rx1 and Rx2 as well as rx1 and rx2 produced an amplicon for all alleles. When the primer pair was combined with 5Rx1F/5Rx1R, respective amplicons were produced, although they were not distinguishable by regular agarose gel electrophoresis. When subjected to a high-resolution DNA melting (HRM) assay, two distinct melting profiles for Rx1 and rx1, respectively, were detected. Triplex PCR-gel electrophoresis and -HRM assay for detection of Rx1, Rx2, and rx1/rx2 were also performed. The efficacy of the HRM assays were validated in potato cultivars/clones with known phenotypes, indicating its potential for high-throughput selection of potato clones/cultivars carrying Rx1 or Rx2. Duplex PCR-HRM assays of over 600 progeny from 12 crosses involving various parents correctly detected the presence or absence of Rx1 in each progeny, allowing accurate prediction of the phenotype. Progeny that tested positive for Rx1 by HRM exhibited ER to PVX whereas progeny that tested negative for Rx1 were susceptible to PVX infection. The genotype of each parent and the possible presence of Nx in two Rx1-possessing parents are also discussed.

scholarly journals Perturbation of nuclear‐cytosolic shuttling of Rx1 compromises extreme resistance and translational arrest of potato virus X transcripts

2021 ◽  
Author(s):  
Manon MS Richard ◽  
Marijn Knip ◽  
Joëlle Schachtschabel ◽  
Machiel S Beijaert ◽  
Frank LW Takken
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Extreme Resistance ◽  
Translational Arrest

Gene-centromere mapping in potato by half-tetrad analysis: map distances of H1, Rx, and Ry and their possible use for ascertaining the mode of 2n-pollen formation

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Marinus Wagenvoort ◽  
Ewa Zimnoch-Guzowska
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Virus X ◽  
2N Pollen ◽  
Tetrad Analysis ◽  
Extreme Resistance ◽  
Second Division Restitution ◽  
Pollen Formation ◽  
Tetraploid Progeny ◽  
Map Distance

Diploids from the tetraploid potato varieties 'Alcmaria' and 'Pansta' and from the tetraploid CPRO genotypes Y66-13-610 and Y66-13-636 were used in half-tetrad analyses to estimate the gene-centromere map distances of the genes Rx, Ry, and H1. Employing tetraploid progeny from 2x (second division restitution) – 4x testcrosses the gene-centromere map distance of H1, conferring resistance to pathotype Ro1 of Globodera rostochiensis, was estimated to be 16.3 centimorgans (cM). For Rχ, conferring extreme resistance to potato virus X (PVX), a map distance of 33.9 cM was estimated. The gene Ry, conferring extreme resistance to potato virus Y (PVY), was estimated to be located 14.2 cM from the centromere. Using the estimated map distance for Rx, it was attempted to determine the mode of 2n-pollen formation in four diploid interspecific hybrids, including the species Solanum tuberosum, Solanum chacoense, Solanum yungasense, and Solanum phureja, by half-tetrad analysis in tetraploid progeny from 4x-2x testcrosses. The mean frequency of 8.7% nulliplex plants for Rx was outside the range of the 95% confidence intervals, for both first division restitution and second division restitution 2n pollen.Key words: nematode resistance, potato virus X resistance, potato virus Y resistance, 2n eggs, gene-centromere mapping, Solanum.

Extreme resistance to infection by potato virus X in genotypes of wild tuber-bearingSolanum species

1991 ◽  
Vol 34 (3) ◽  
pp. 317-324 ◽  
Author(s):  
Alejandro Carlos Tozzini ◽  
María Fernanda Ceriani ◽  
María Verónica Saladrigas ◽  
H. Esteban Hopp
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Extreme Resistance ◽  
Resistance To Infection

Evaluation of extreme resistance genes of Potato virus X (Rx1 and Rx2) in different potato genotypes

2018 ◽  
Vol 125 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Hassan O. Shaikhaldein ◽  
Borbala Hoffmann ◽  
Ibrahim A. Alaraidh ◽  
Dalia G. Aseel
Keyword(s):  
Potato Virus ◽  
Resistance Genes ◽  
Potato Virus X ◽  
Extreme Resistance

Yukon Nugget: a Mid-Season Yellow Skin, Yellow Flesh Specialty Potato with Extreme Resistance to Potato Virus X

2016 ◽  
Vol 93 (6) ◽  
pp. 602-608 ◽  
Author(s):  
V. Sathuvalli ◽  
C. R. Brown ◽  
S. Yilma ◽  
B. A. Charlton ◽  
C. C. Shock ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Extreme Resistance ◽  
Yellow Flesh

Reactive oxygen species contribute to symptomless, extreme resistance to Potato virus X in tobacco

2021 ◽  
Author(s):  
Lóránt Király ◽  
Réka Albert ◽  
Orsolya Zsemberi ◽  
Ildikó Schwarczinger ◽  
Yasser Mohamed Hafez ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Potato Virus ◽  
Reactive Oxygen ◽  
Potato Virus X ◽  
Defense Responses ◽  
Oxygen Species ◽  
Extreme Resistance ◽  
Hypersensitive Resistance ◽  
Resistance Response ◽  
Regulator Genes

Here we show that in tobacco (Nicotiana tabacum cv. Samsun NN Rx1) the development of Rx1 gene-mediated, symptomless, extreme resistance to Potato virus X (PVX) is preceded by an early, intensive accumulation of the reactive oxygen species (ROS) superoxide (O2.-), evident between 1-6 hours after inoculation and associated with enhanced NADPH oxidase activities. This suggests a direct contribution of this ROS to virus restriction during symptomless, extreme resistance. Superoxide inhibition in PVX-inoculated leaves by infiltration of antioxidants (superoxide dismutase, SOD and catalase, CAT) partially suppresses extreme resistance parallel with the appearance of localized leaf necrosis resembling a hypersensitive resistance response (HR). F1 progeny from crosses of Rx1 and ferritin-overproducer (deficient in production of the ROS OH.) tobaccos also display a suppressed extreme resistance to PVX, since significantly increased virus levels are coupled to HR, suggesting a role of the hydroxyl radical (OH.) in this symptomless antiviral defense. In addition, treatment of PVX-susceptible tobacco with a superoxide-generating agent (riboflavin/methionine) results in HR-like symptoms and reduced PVX titers. Finally, by comparing defense responses during PVX-elicited symptomless, extreme resistance and HR-type resistance elicited by Tobacco mosaic virus (TMV) we conclude that defense reactions typical of an HR (e.g. induction of cell death/ROS-regulator genes and antioxidants) are early and transient in the course of extreme resistance. Our results demonstrate the contribution of early accumulation of ROS (superoxide, OH.) in limiting PVX replication during symptomless extreme resistance and support earlier findings that virus-elicited HR represents a delayed, slower resistance response than symptomless, extreme resistance.

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