Recovery of transgenic rice plants expressing the rice dwarf virus outer coat protein gene (S8)

1997 ◽  
Vol 94 (3-4) ◽  
pp. 522-527 ◽  
Author(s):  
H. H. Zheng ◽  
Y. Li ◽  
Z. H. Yu ◽  
W. Li ◽  
M. Y. Chen ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Rice ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Rice Dwarf Virus ◽  
Outer Coat ◽  
Rice Plants ◽  
Transgenic Rice Plants

scholarly journals Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

2010 ◽  
Vol 25 (6) ◽  
pp. 401-408 ◽  
Author(s):  
Guo-cheng Fan ◽  
Fang-luan Gao ◽  
Tai-yun Wei ◽  
Mei-ying Huang ◽  
Li-yan Xie ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Outer Coat ◽  
Rice Gall Dwarf Virus

scholarly journals Assembly of Double-Shelled, Virus-Like Particles in Transgenic Rice Plants Expressing Two Major Structural Proteins of Rice Dwarf Virus

2000 ◽  
Vol 74 (20) ◽  
pp. 9808-9810 ◽  
Author(s):  
Honghong Zheng ◽  
Li Yu ◽  
Chunhong Wei ◽  
Dongwei Hu ◽  
Yunping Shen ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Structural Integrity ◽  
Core Protein ◽  
Dwarf Virus ◽  
Structural Proteins ◽  
Rice Dwarf Virus ◽  
Virus Like Particles ◽  
Rice Plants ◽  
Transgenic Rice Plants ◽  
Core Proteins

ABSTRACT Rice dwarf virus (RDV) is a double-shelled particle that contains a major capsid protein (P8), a major core protein (P3), several minor core proteins, and viral genomic double-stranded RNA. Coexpression of P8 and P3 in transgenic rice plants resulted in formation of double-shelled, virus-like particles (VLPs) similar to the authentic RDV particles. The VLPs were not detected in transgenic rice plant cells expressing P8 alone. This in vivo result suggests that P8 interacted with P3 and that these two proteins provide the structural integrity required for the formation of VLPs in rice cells independently of other structural proteins, nonstructural proteins, or viral genomic double-stranded RNAs.

scholarly journals First Report of Raspberry bushy dwarf virus in Rubus multibracteatus from China

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 603-603 ◽  
Author(s):  
C. J. Chamberlain ◽  
J. Kraus ◽  
P. D. Kohnen ◽  
C. E. Finn ◽  
R. R. Martin
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Black Raspberry ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Plant Pathol

Raspberry bushy dwarf virus (RBDV), genus Idaeovirus, has been reported in commercial Rubus spp. from North and South America, Europe, Australia, New Zealand, and South Africa. Infection can cause reduced vigor and drupelet abortion leading to crumbly fruit and reduced yields (3,4). In recent years, Rubus germplasm in the form of seed, was obtained on several collection trips to The People's Republic of China to increase the diversity of Rubus spp. in the USDA-ARS National Clonal Germplasm Repository, (Corvallis, OR). Before planting in the field, seedlings were tested for the presence of RBDV, Tomato ringspot virus, and Tobacco streak virus using triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) (antiserum produced by R. R. Martin). One symptomless plant of R. multibracteatus H. Lev. & Vaniot (PI 618457 in USDA-ARS GRIN database), from Guizhou province in China, tested positive for RBDV (RBDV-China). After mechanical transmission on Chenopodium quinoa Willd., this isolate produced typical symptoms of RBDV (3). To determine if RBDV-China was a contaminant during the handling of the plants, or if the source was a seedborne virus, the coat protein gene was sequenced and compared to published sequences of RBDV. RNA was extracted from leaves of R. multibracteatus and subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers that flank the coat protein gene. Products from four separate PCR reactions were sequenced directly or were cloned into the plasmid vector pCR 2.1 (Invitrogen, Carlsbad, CA) and then sequenced. The coding sequence of the coat protein gene of RBDV-China was 87.5% (722/825) identical to that isolated from black raspberry (Genbank Accession No. s55890). The predicted amino acid sequences were 91.6% (251/274) identical. Previously, a maximum of five amino acid differences had been observed in the coat proteins of different RBDV strains (1). The 23 differences observed between RBDV-China and the isolate from black raspberry (s55890) confirm that the RBDV in R. multibracteatus is not a greenhouse contaminant but is indeed a unique strain of RBDV. In addition, monoclonal antibodies (MAbs) to RBDV (2) were tested against RBDV-China. In these tests, MAb D1 did not detect RBDV-China, whereas MAb R2 and R5 were able to detect the strain. This is the first strain of RBDV that has been clearly differentiated by MAbs using standard TAS-ELISA tests. Although RBDV is common in commercial Rubus spp. worldwide, to our knowledge, this is the first report of RBDV in R. multibracteatus, and the first report of RBDV from China. The effects of this new strain of RBDV could be more or less severe, or have a different host range than previously studied strains. It is more divergent from the type isolate than any other strain that has been studied to date. Phylogenetic analysis of coat protein genes of RBDV may be useful in understanding the evolution and spread of this virus. References: (1) A. T. Jones et al. Eur. J. Plant Pathol. 106:623, 2000. (2) R. R. Martin. Can. J. Plant. Pathol. 6:264, 1984. (3) A. F. Murant. Raspberry Bushy Dwarf. Page 229 in: Virus Diseases of Small Fruits. R. H. Converse, ed. U.S. Dep. Agric. Agric. Handb. 631, 1987. (4) B. Strik and R. R. Martin. Plant Dis. 87:294, 2003.

Sequence and identification of the barley yellow dwarf virus coat protein gene

Virology ◽  
1988 ◽  
Vol 165 (1) ◽  
pp. 306-309 ◽  
Author(s):  
W.A. Miller ◽  
P.M. Waterhouse ◽  
A.A. Kortt ◽  
W.L. Gerlach
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Virus Coat Protein ◽  
Yellow Dwarf Virus ◽  
Virus Coat

scholarly journals In Vitro Expression and Antibody Preparation of Rice black-streaked dwarf virus Coat Protein Gene

2016 ◽  
Vol 22 (1) ◽  
pp. 32-37
Author(s):  
Bong Choon Lee ◽  
Sang-Yun Cho ◽  
Ju Young Bae ◽  
Sang Min Kim ◽  
Dong Bum Shin ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Antibody Preparation ◽  
In Vitro Expression ◽  
Virus Coat Protein ◽  
Virus Coat
Sign in / Sign up

Export Citation Format

Share Document