scholarly journals Assignment of DNA markers to Nicotiana sylvestris chromosomes using monosomic alien addition lines

1997 ◽  
Vol 94 (3-4) ◽  
pp. 331-337 ◽  
Author(s):  
D. F. Suen ◽  
C. K. Wang ◽  
R. F. Lin ◽  
Y. Y. Kao ◽  
F. M. Lee ◽  
...  
Author(s):  
Puneet Kaur Mangat ◽  
Junghyun Shim ◽  
Ritchel B. Gannaban ◽  
Joshua J. Singleton ◽  
Rosalyn B. Angeles-Shim

Abstract Key message Alien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines of S. lycopersicoides confer novel phenotypes with commercial and agronomic value in tomato breeding. Abstract Solanum lycopersicoides is a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) of S. lycopersicoides to establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wild S. lycopersicoides parent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more than S. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.


1987 ◽  
Vol 50 (2) ◽  
pp. 91-97 ◽  
Author(s):  
B. P. Forster ◽  
S. M. Reader ◽  
S. A. Forsyth ◽  
R. M. D. Koebner ◽  
T. E. Miller ◽  
...  

SummarySix wheat/Agropyron intermedium addition lines are described on the basis of their phenotype and biochemical markers. An assessment of homoeology of each addition chromosome is made. Chromosome morphology, plant phenotype, isozyme and protein studies are compared with similar data for other wheat/alien addition lines and other members of the Triticeae. These comparisons give consistent results and it is concluded that addition lines L1, L2, L3, L4, L5 and L7 carry Agropyron chromosomes of homoeologous groups 7, 3, 1, 4, 5 and 6 respectively. This agrees with previously published work with one exception: the L5 chromosome belongs to homoeologous group 5 and not group 2 as proposed by Figueiras et al. (1986).


2010 ◽  
Vol 130 (1) ◽  
pp. 60-66 ◽  
Author(s):  
D. Sarr ◽  
J-M. Lacape ◽  
M. Rodier-Goud ◽  
J-M. Jacquemin ◽  
H. Benbouza ◽  
...  

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 236-240 ◽  
Author(s):  
G. Hueros ◽  
J. M. Gonzalez ◽  
J. C. Sanz ◽  
E. Ferrer

Gliadin protein components from Aegilops longissima were separated by two-dimensional electrophoresis. No equivalents for α-gliadin were noted. Addition and substitution lines of Ae. longissima in Triticum aestivum 'Chinese Spring' allowed the identification of homoeologous Gli-1 and Gli-2 loci in Ae. longissima chromosomes 1S1 and 6S1. The chromosomal constitution of the alien addition lines was ascertained by C-banding. In addition, C-banding analysis revealed that the Ae. longissima addition set was incomplete as only six distinct addition lines were identified. No evidence for structural modifications between the alien chromosomes in the lines and their Ae. longissima counterparts was found.Key words: gliadins, C-banding, gene location, Aegilops longissima, wheat.


2007 ◽  
Vol 82 (5) ◽  
pp. 403-408 ◽  
Author(s):  
Monika Garg ◽  
Hala M. M. Elamein ◽  
Hiroyuki Tanaka ◽  
Hisashi Tsujimoto

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