Molecular mapping of the potato virus Y resistance gene Rysto in potato

1997 ◽  
Vol 94 (2) ◽  
pp. 198-203 ◽  
Author(s):  
G. Brigneti ◽  
J. Garcia-Mas ◽  
D. C. Baulcombe
2011 ◽  
Vol 60 (6) ◽  
pp. 1048-1054 ◽  
Author(s):  
C. Lacroix ◽  
L. Glais ◽  
J.-L. Verrier ◽  
C. Charlier ◽  
C. Lorencetti ◽  
...  

Euphytica ◽  
2006 ◽  
Vol 149 (3) ◽  
pp. 367-372 ◽  
Author(s):  
Masatoshi Sato ◽  
Kazuko Nishikawa ◽  
Kuninori Komura ◽  
Kazuyoshi Hosaka

2018 ◽  
Vol 67 (7) ◽  
pp. 1629-1635 ◽  
Author(s):  
T. Ohki ◽  
M. Sano ◽  
K. Asano ◽  
T. Nakayama ◽  
T. Maoka

2014 ◽  
Vol 65 (4) ◽  
pp. 1095-1109 ◽  
Author(s):  
Š. Baebler ◽  
K. Witek ◽  
M. Petek ◽  
K. Stare ◽  
M. Tušek-Žnidarič ◽  
...  

2002 ◽  
Vol 32 (6) ◽  
pp. 1067-1075 ◽  
Author(s):  
Sandrine Ruffel ◽  
Marie-Hélène Dussault ◽  
Alain Palloix ◽  
Benoît Moury ◽  
Abdelhafid Bendahmane ◽  
...  

Plant Disease ◽  
2006 ◽  
Vol 90 (5) ◽  
pp. 559-566 ◽  
Author(s):  
P. M. Baldauf ◽  
S. M. Gray ◽  
K. L. Perry

A survey of six potato viruses, Potato virus A (PVA), Potato virus M (PVM), Potato virus S(PVS), Potato virus X (PVX), Potato virus Y (PVY), and Potato leafroll virus (PLRV), was conducted in New York and Maine during 2002 and 2003. Leaf samples were tested by enzyme-linked immunosorbent assay and PVY-positive samples were further tested to determine whether a necrotic strain of PVY (PVYN) or a strain able to induce necrosis in tobacco and in potato tubers (PVYNTN) were present. In both years, PVY and PVS were identified in a majority of the samples, and mixed infections predominated in 83% of the symptomatic leaves in 2002. Of the total 394 PVY-positive samples, 3 reacted with monoclonal antibody (MAb) 1F5 and caused veinal necrosis (VN) in tobacco. Two of these isolates caused tuber necrosis in the potato cv. Yukon Gold. Three PVY isolates reacted with MAb 1F5 but did not cause VN in tobacco, and two caused VN but did not react with MAb 1F5. None of these eight isolates were able to overcome the Ry resistance gene in the potato cultivar Eva, but several were able to overcome the Ny resistance gene found in Allegany. PVYN isolates were not widespread in the northeastern United States; however, several PVY isolates differed from both PVYN and the ordinary strain of PVY and may represent strain recombinants.


Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 832
Author(s):  
Brittney M. Caruana ◽  
Brendan C. Rodoni ◽  
Fiona Constable ◽  
Anthony T. Slater ◽  
Noel O. I. Cogan

Potato is an important food crop worldwide and is grown in a large number of countries. As such, the crop is under disease pressures and the need for selecting disease resistance genes during breeding programs is essential. Of particular importance within Australia and other parts of the world is the potyvirus, Potato virus Y (PVY). In this paper, three commonly used PVY resistance markers, M45, RYSC3 and M6, were evaluated using existing genomic resources and phenotypic data from the Australian potato breeding program to identify a region where the PVY resistance gene, Ryadg may reside. A region of Chromosome XI was investigated, and a cluster of disease resistance genes was identified that the resistance gene Ryadg is suspected to reside within. Protein characterization was also performed on the putative resistant gene. A specific variant that had complete association with the resistance gene was identified and a single nucleotide polymorphism (SNP) assay was designed to avoid dissociation of marker and gene in future breeding programs. This SNP marker (SNP37279) was validated as a Kompetitive Allele-specific PCR (KASP) genotyping assay and was found to perform more accurately than all previously used markers for detecting Ryadg.


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