Molecular mapping of chromosome segments introgressed from Solanum lycopersicoides into cultivated tomato (Lycopersicon esculentum)

2000 ◽  
Vol 100 (2) ◽  
pp. 232-241 ◽  
Author(s):  
R. T. Chetelat ◽  
V. Meglic
Keyword(s):  
Lycopersicon Esculentum ◽  
Molecular Mapping ◽  
Solanum Lycopersicoides ◽  
Chromosome Segments

A library of Solanum lycopersicoides introgression lines in cultivated tomato

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 685-697 ◽  
Author(s):  
Michael A Canady ◽  
Vladimir Meglic ◽  
Roger T Chetelat
Keyword(s):  
Lycopersicon Esculentum ◽  
Segregation Distortion ◽  
Germ Line ◽  
Introgression Lines ◽  
Primary Group ◽  
Solanum Lycopersicoides ◽  
Minimum Number ◽  
Map Resolution ◽  
Strong Segregation ◽  
Chromosome Segments

A set of introgression lines (ILs), containing individual chromosome segments from the wild nightshade Solanum lycopersicoides bred into the genetic background of cultivated tomato (Lycopersicon esculentum), has been developed. A primary group of 56 lines was selected for maximum representation of the S. lycopersicoides genome (~96% of the total map units), homozygosity, and a minimum number of introgressed segments per line. A secondary set of 34 lines provides increased map resolution in certain regions. Approximately 34% of the lines were sterile in the homozygous condition, but could be maintained by heterozygotes. To facilitate identification of segregating ILs, restriction fragment length polymorphism probes were converted to higher throughput cleaved amplified polymorphic sequence markers, which supplement allozyme and morphological loci. Strong segregation distortion was observed in F2 progeny of heterozygous ILs, with an excess of L. esculentum alleles in most regions. For introgressions on distal chromosome 1L, a preferential transmission of S. lycopersicoides alleles was observed in the male germ line. Homozygous ILs generally yielded less seed from self pollination than corresponding heterozygotes, indicating that sterility effects were recessive. This IL library provides a novel resource for genetic studies of traits found in S. lycopersicoides.Key words: Lycopersicon esculentum, Solanum lycopersicum, Solanum lycopersicoides, segregation distortion, alien introgression.

scholarly journals Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)

2021 ◽  
Author(s):  
Puneet Kaur Mangat ◽  
Junghyun Shim ◽  
Ritchel B. Gannaban ◽  
Joshua J. Singleton ◽  
Rosalyn B. Angeles-Shim
Keyword(s):  
Chromosome Complement ◽  
Introgression Lines ◽  
Addition Lines ◽  
Solanum Lycopersicoides ◽  
Drought Tolerant ◽  
Use Efficiency ◽  
Tomato Breeding ◽  
Chromosome Segments ◽  
Alien Addition Lines

Abstract Key message Alien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines of S. lycopersicoides confer novel phenotypes with commercial and agronomic value in tomato breeding. Abstract Solanum lycopersicoides is a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) of S. lycopersicoides to establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wild S. lycopersicoides parent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more than S. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.

scholarly journals A Chlorophyll Fluorescence Screening Test to Evaluate Chilling Tolerance in Tomato

HortScience ◽  
1990 ◽  
Vol 25 (3) ◽  
pp. 334-339 ◽  
Author(s):  
Mark A. Walker ◽  
Dale M. Smith ◽  
K. Peter Pauls ◽  
Bryan D. McKersie
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Lycopersicon Esculentum ◽  
Temperature Stress ◽  
Low Temperatures ◽  
Screening Test ◽  
Full Range ◽  
Chilling Tolerance ◽  
Solanum Lycopersicoides ◽  
Commercial Cultivars

The chilling tolerance of commercial Lycopersicon esculentum cultivars (H2653, H722), Solanum lycopersicoides, an F1 hybrid of S. lycopersicoides × Sub-Arctic Maxi, and 25 BC2F2 lines of L. hirsutum × H722 (backcrossed twice to H722) was evaluated using a chlorophyll fluorescence assay. The ratio of the initial to the peak fluorescence (Fo: Fp) measured from fully expanded leaves was chosen as an indicator of plant health. Chilling induced an increase in Fo: Fp that was correlated with the sensitivity of the plant to low-temperature stress. Values of Fo: Fp remained low for cold-treated S. lycopersicoides and the F1 hybrid, which showed few symptoms of chilling-related damage, whereas the commercial cultivars, which were essentially intolerant to low temperatures, had large increases in Fo: Fp. A full range of Fo: Fp values was measured in the 25 BC2F2 lines, indicating that some chilling tolerance from the L. hirsutum parent was expressed by plants in these populations.

Isozyme analysis, chromosome pairing, and fertility of Lycopersicon esculentum × Solanum lycopersicoides diploid backcross hybrids

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Roger T. Chetelat ◽  
Charles M. Rick ◽  
Joseph W. DeVerna
Keyword(s):  
Lycopersicon Esculentum ◽  
Chromosome Pairing ◽  
Linkage Mapping ◽  
Intergeneric Hybridization ◽  
Morphological Markers ◽  
Stainable Pollen ◽  
Mendelian Segregation ◽  
Solanum Lycopersicoides ◽  
Novel Traits ◽  
Backcross Hybrids

The wild nightshade Solanum lycopersicoides possesses potentially valuable traits for tomato (Lycopersicon esculentum) improvement. Male sterility of the intergenic F1 hybrid and its stylar rejection of tomato pollen have until recently prevented introgression. In the work herein described, ovule fertility was detected in the F1 hybrid following pollinations with S. lycopersicoides. Pollinations of the F1 with L. pennellii derived bridging hybrids resulted in a BC1 population of 65 plants. BC2 seed was obtained from most BC1 individuals. Male fertility of the BC1 plants ranged from 0 to 66% acetocarmine-stainable pollen. Of the 43 plants scored, 41 were diploids (2n = 24) and 2 were aneuploids (2n = 25 and 2n = 26). Chromosome pairing of the BC1 at diakinesis and metaphase I averaged 11.1 bivalents per pollen mother cell. BC1 and BC2 plants were scored for 19 isozyme and morphological markers covering 10 of the 12 S. lycopersicoides chromosomes. Significant deviations from Mendelian segregation were observed for seven loci, with an excess of homozygotes (+/+) in all cases. Wa, which controls the white anther trait of S. lycopersicoides, was mapped to within 15 centiMorgans (cM) of Got-4, while Mdh-4 was closely linked to Pgi-1 (<2 cM). Plants heterozygous for the S. lycopersicoides allele (+/s) at Dia-2, Skdh-1, Wa, and Aco-1 showed significantly lower pollen stainability than homozygotes (+/+). Skdh-1 and Got-3 heterozygotes had less chromosome pairing at meiosis than homozygotes. These hybrids will permit the introgression of novel traits from S. lycopersicoides into tomato.Key words: tomato, segregation, linkage mapping, quantitative trait loci, intergeneric hybridization.

scholarly journals Fertile Somatic Hybrids between F1(Lycopersicon esculentum*L. peruvianum var. humifusum) and Solanum lycopersicoides.

1997 ◽  
Vol 47 (4) ◽  
pp. 327-333
Author(s):  
Asako Matsumoto ◽  
Shigeru Imanishi ◽  
Monzur Hossain ◽  
Alice Escalante ◽  
Hiroaki Egashira
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Solanum Lycopersicoides

Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides

1986 ◽  
Vol 71 (5) ◽  
pp. 691-697 ◽  
Author(s):  
L. W. Handley ◽  
R. L. Nickels ◽  
M. W. Cameron ◽  
P. P. Moore ◽  
K. C. Sink
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrid ◽  
Solanum Lycopersicoides ◽  
Hybrid Plants

Identification, transmission, and cytological behavior of Solanum lycopersicoides Dun. monosomic alien addition lines in tomato (Lycopersicon esculentum Mill.)

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 40-50 ◽  
Author(s):  
Roger T Chetelat ◽  
Charles M Rick ◽  
Pedro Cisneros ◽  
Kevin B Alpert ◽  
Joseph W DeVerna
Keyword(s):  
Lycopersicon Esculentum ◽  
Extra Chromosome ◽  
Addition Lines ◽  
Solanum Lycopersicoides ◽  
Visual Markers ◽  
Total Progeny ◽  
Fruit Characters ◽  
Sexual Crosses ◽  
Homoeologous Chromosomes ◽  
Alien Addition Lines

The wild nightshade Solanum lycopersicoides possesses a number of disease- and arthropod-resistance and environmental stress tolerance traits of potential value for the cultivated tomato, Lycopersicon esculentum. F1 hybrids are readily obtained by sexual crosses, but backcrosses to tomato are hindered by sterility and incompatibility barriers. In the present studies, intergeneric sesquidiploid hybrids (2 genomes of L. esculentum and 1 of S. lycopersicoides) were pollinated with Lycopersicon pennellii derived bridging lines, yielding diploid, aneuploid (up to 2n + 6), and polyploid (3x, 4x) progeny. After diploids (58% of total progeny), the group most frequently obtained was 2n + 1 (31%), which included all 12 possible monosomic alien addition lines (MAALs) containing a single extra chromosome from S. lycopersicoides in a tomato background. MAAL groups were identified with a combination of allozyme, RFLP, and monogenic visual markers. Transmission rates of the extra chromosomes in MAALs varied from 0%, for chromosome 6, to 24%, for chromosome 10. At diakinesis and metaphase I of meiosis, the alien chromosome was usually unpaired (in an average of 90% of PMCs), but association of homoeologous chromosomes was observed in each of the 12 MAAL groups (in an average of 5.7% of PMCs). The MAALs showed a striking morphological resemblance to the corresponding tomato primary trisomic. In addition, each MAAL expressed S. lycopersicoides specific leaf, flower, and (or) fruit characters. This set of MAALs, the first of its kind in Lycopersicon, is expected to be useful for a variety of gene-mapping and introgression studies.

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