Identification of larch species (Larix decidua, Larix kaempferi and Larix X eurolepis) and estimation of hybrid fraction in seed lots by RAPD fingerprints

2000 ◽  
Vol 100 (1) ◽  
pp. 71-74 ◽  
Author(s):  
D. Scheepers ◽  
M. -C. Eloy ◽  
M. Briquet
2017 ◽  
Vol 14 (7) ◽  
pp. 1341-1349 ◽  
Author(s):  
Nova D. Doyog ◽  
Young Jin Lee ◽  
Sun Joo Lee ◽  
Jin Taek Kang ◽  
Sung Yong Kim

2020 ◽  
Vol 24 (6) ◽  
pp. 34-43
Author(s):  
M.D. Merzlenko ◽  
◽  
P.G. Melnik ◽  
Yu.B. Glazunov ◽  
A.A. Kozhenkova ◽  
...  

The results of the cultivation of Scots pine (Pinus sylvestris L.) in the provenance trial in the Serebryanoborsky experimental forest district of the Institute of Forestry of the Russian Academy of Sciences are summarized. The silvicultural effect was assessed by a comprehensive indicator of the feasibility of introducing specific pine conventions. It was established that the use of pine seeds of exclusively local provenances cannot be considered justified. In the vast area of pine there are very remote populations of a local nature, the seed of which, when moved, can be successfully used to create highly productive artificial stands. The 68 year old geographical plantations of larch growth and production rates of 18 climotypes of 14 larch species were compared. These species are Polish larch (Larix polonica Racib.), European larch (Larix decidua Mill. f. Sudetica), Larix sukaczewii Dylis, Siberian larch (Larix sibirika Ledeb.), Larix cajanderi Mayr., Larix gmelinii Rupr., Larix Czekanovskii Szaf, Larix amurensis Kolesn., Olgan larch (Larix olgensis Henry), Japanese larch (Larix kaempferi (Lamb.) Carrière), Larix kurilensis Maur., Larix principis Rupprechtii Maur., Larix potaninii Bat, American larch (Larix laricina (Duroi) K. Koch). Polish larch, Japanese larch, European larch and Olgan larch have the best growth rate results. Climotypes from Siberia and American larch showed worst results. Polish larch (812 m3/ha) and Japanese (804 m3/ha) larch from the Southern Sakhalin have the highest stem volume. The final estimations showed that the climotypes of Polish larch, European larch from Sudetes and Japanese larch from the Southern Sakhalin are suitable for introduction in the Moscow Region. The climotypes from the Far East, namely Larix amurensis Kolesn., Olgan larch and Larix kurilensis Maur., had positive silvicultural effect. The inland climotypes from Asia together with American larch showed negative results.


2008 ◽  
Vol 84 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Fabienne Colas ◽  
Martin Perron ◽  
Denise Tousignant ◽  
Carol Parent ◽  
Monique Pelletier ◽  
...  

Hybrid larch ([Larix × marschlinsii Coaz], HL) is sought after by the forest industry because of its growth characteristics, excellent wood quality and disease resistance. However, the difficulty in obtaining HL seeds limits the production of seedlings for reforestation. Both European larch (Larix decidua Mill.) and Japanese larch (Larix kaempferi [Lamb.] Carrière) produce few seeds and the time lag in their flowering phenology complicates their natural pollination. We have developed a novel type of sheltered seed orchard, dedicated to the production of HL seeds from grafts grown in pots. Inverting the arches of a conventional tunnel provides a sturdy structure which is easy to maintain. Because of its height, the tunnel can accommodate the rapid growth of the trees, which reduces the need for pruning. The tunnels are covered in the winter and spring. In the winter, the covers prevent snow accumulation. In the spring, they create a "greenhouse effect" inside the tunnels, accelerating flower development, preventing pollen contamination from trees outside the tunnels and protecting the flowers from late spring frosts. Over the past five years, flowering has been regular and abundant. Bagging the crowns for pollen harvest is cost-effective and yields large quantities of high quality pollen. Pollination operations are conducted using an electrostatic pistol. Because of the large number of available clones, large quantities of seeds with a high genetic variability can be produced at a competitive cost. In 2006, this seed orchard concept was implemented operationally at the Berthier forest nursery (Québec, Canada). Key words: Japanese larch (Larix kaempferi [Lamb.] Carrière), European larch (Larix decidua Mill.), seed production, electrostatic pollination, indoor seed orchard


Author(s):  
Satoshi Kitaoka ◽  
Laiye Qu ◽  
QiaoZhi Mao ◽  
Yoko Watanabe ◽  
Makoto Watanabe ◽  
...  

Ground-level ozone (O3) increases in the northeastern part of Eurasia, where larch species are dominant trees and have been planted intensively. The Japanese larch (Larix kaempferi; hereafter larch) is classified as the tree species most of its sensitive to O3 based on data from long-shoot needles of seedlings. This criterion should be reconsidered based on O3 uptake, which is strongly depended on stomatal conductance (Gs) of adult trees through the difference in relevant needle traits. Because Gs is closely correlated with photosynthetic activity, we measured the in situ seasonal and yearly change in photosynthetic function, needle mass per area (LMA) and nitrogen (N) content of both short- and long-shoot needles of the canopy of larch trees over successive 3 years. No difference was observed in the in light saturated photosynthetic rate at ambient CO2 (Psat) between needles of hetero types of shoot in the latter part of the growing season, but clear differences were found within a specific year, indicating that seasonal changes in climatic factors determine Psat in the long term. The Psat-N relations differed significantly between the needle types. However, Psat at saturated CO2 (Pmax)-N was quite similar, implying that the CO2 diffusion difference between short- and long-shoots is responsible for the differing Psat -N. In conclusion, seasonal and yearly variations in photosynthetic capacity are mainly determined mainly by climatic variations, whereas shoot type determined the traits of photosynthetic N utilization as well as Gs regulation.


2021 ◽  
Vol 12 ◽  
Author(s):  
Tony Heitkam ◽  
Luise Schulte ◽  
Beatrice Weber ◽  
Susan Liedtke ◽  
Sarah Breitenbach ◽  
...  

In eukaryotic genomes, cycles of repeat expansion and removal lead to large-scale genomic changes and propel organisms forward in evolution. However, in conifers, active repeat removal is thought to be limited, leading to expansions of their genomes, mostly exceeding 10 giga base pairs. As a result, conifer genomes are largely littered with fragmented and decayed repeats. Here, we aim to investigate how the repeat landscapes of two related conifers have diverged, given the conifers’ accumulative genome evolution mode. For this, we applied low-coverage sequencing and read clustering to the genomes of European and Japanese larch, Larix decidua (Lamb.) Carrière and Larix kaempferi (Mill.), that arose from a common ancestor, but are now geographically isolated. We found that both Larix species harbored largely similar repeat landscapes, especially regarding the transposable element content. To pin down possible genomic changes, we focused on the repeat class with the fastest sequence turnover: satellite DNAs (satDNAs). Using comparative bioinformatics, Southern, and fluorescent in situ hybridization, we reveal the satDNAs’ organizational patterns, their abundances, and chromosomal locations. Four out of the five identified satDNAs are widespread in the Larix genus, with two even present in the more distantly related Pseudotsuga and Abies genera. Unexpectedly, the EulaSat3 family was restricted to L. decidua and absent from L. kaempferi, indicating its evolutionarily young age. Taken together, our results exemplify how the accumulative genome evolution of conifers may limit the overall divergence of repeats after speciation, producing only few repeat-induced genomic novelties.


Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 837-848 ◽  
Author(s):  
Jean-François Trontin ◽  
Catherine Grandemange ◽  
Jean-Michel Favre

The 5S ribosomal DNA unit structure and organization have been investigated in Larix decidua and Larix kaempferi using selective amplification of gene and spacer, sequence analysis and homologous probe hybridization. Two highly divergent unit size classes of approximately 650 and 870 bp were detected in both species. Sequence analysis in Larix decidua revealed that length variations occur in the middle spacer region and are the result of duplications (in the long spacers) and considerable sequence heterogeneity. Conversely, the transcribed region is of uniform length (120 bp), and the nucleotide sequence of one Larix decidua clone is similar to that reported for other gymnosperms. Sequence comparison of the larch spacers with two other Pinaceae species (Pinus radiata and Picea glauca) showed that the 5' and 3' regions flanking the gene (40 and 60 bp, respectively) are quite conserved, suggesting a regulatory role. Moreover, a small element of about 70 bp located in the middle spacer region was found to be common to the larch long units and the six Pinus radiata spacer clones previously sequenced (64% sequence identity). The short and long unit size classes are mainly organized in composite tandem array(s) with evidence of extensive zones of strict alternation in both species. Mechanisms underlying this unusual association of divergent units in larch 5S rDNA arrays are discussed.Key words: 5S rRNA genes, spacer variations, cluster organization, sequence comparison, Gymnosperms.


1994 ◽  
Vol 9 (1-4) ◽  
pp. 214-217 ◽  
Author(s):  
Andrzej Lewandowski ◽  
Teijo Nikkanen ◽  
Jarosław Burczyk

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