Cloning of six cherry self-incompatibility alleles and development of allele-specific PCR detection

2001 ◽  
Vol 102 (6-7) ◽  
pp. 1046-1055 ◽  
Author(s):  
T. Sonneveld ◽  
T. P. Robbins ◽  
R. Bošković ◽  
K. R. Tobutt
Keyword(s):  
Pcr Detection ◽  
Self Incompatibility ◽  
Specific Pcr ◽  
Allele Specific ◽  
Incompatibility Alleles ◽  
Allele Specific Pcr

scholarly journals Self-Incompatibility Alleles in Iranian Pear Cultivars

2019 ◽  
Author(s):  
Maryam Bagheri ◽  
Ahmad Ershadi
Keyword(s):  
Rapid Method ◽  
Pcr Amplification ◽  
Self Incompatibility ◽  
Specific Primers ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Incompatibility Alleles ◽  
Consensus Primers ◽  
Allele Specific Pcr

AbstractIn the present study, the S-alleles of eighteen pear cultivars, (including fourteen cultivars planted commercially in Iran and four controls) are determined. 34 out of 36 S-alleles are detected using nine allele-specific primers, which are designed for amplification of S101/S102, S105, S106, S107, S108, S109, S111, S112 and S114, as well as consensus primers, PycomC1F and PycomC5R. S104, S101 and S105 were the most common S-alleles observed, respectively, in eight, seven and six cultivars. In 16 cultivars, (‘Bartlett’ (S101S102), ‘Beurre Giffard’ (S101S106), ‘Comice’ (S104S105), ‘Doshes’ (S104S107), ‘Koshia’ (S104S108), ‘Paskolmar’ (S101S105), ‘Felestini’ (S101S107), ‘Domkaj’ (S104S120), ‘Ghousi’ (S104S107), ‘Kaftar Bache’ (S104S120), ‘Konjoni’ (S104S108), ‘Laleh’ (S105S108), ‘Natanzi’ (S104S105), ‘Sebri’ (S101S104), ‘Se Fasleh’ (S101S105) and ‘Louise Bonne’ (S101S108)) both alleles are identified but in two cultivars, (‘Pighambari’ (S105) and ‘Shah Miveh Esfahan’ (S107)) only one allele is recognized. It is concluded that allele-specific PCR amplification can be considered as an efficient and rapid method to identify S-genotype of Iranian pear cultivars.

Evolutionary pharmacogenetics of CYP2D6 in Ngawbe Guaymi of Panama: allele-specific PCR detection of the CYP2D6B allele and RFLP analysis

1993 ◽  
Vol 3 (5) ◽  
pp. 231-238 ◽  
Author(s):  
Luc??a F. Jorge ◽  
Tom??s D. Arias ◽  
Ulrich Griese ◽  
Daniel W. Nebert ◽  
Michel Eichelbaum
Keyword(s):  
Rflp Analysis ◽  
Pcr Detection ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

scholarly journals Identification of Self-incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis

2014 ◽  
Vol 46 (4) ◽  
pp. 364-371
Author(s):  
Kang Hee Cho ◽  
Jeong-Hee Kim2 ◽  
Jung Woo Lee ◽  
Soon-Il Kwon ◽  
Jong Taek Park ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
Specific Pcr ◽  
Allele Specific ◽  
S Allele ◽  
Allele Specific Pcr

Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis

2000 ◽  
Vol 101 (3) ◽  
pp. 344-349 ◽  
Author(s):  
M. Tamura ◽  
K. Ushijima ◽  
H. Sassa ◽  
H. Hirano ◽  
R. Tao ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

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