The low-density lipoprotein receptor gene family: multiple roles in lipid metabolism

1999 ◽  
Vol 77 (3) ◽  
pp. 306-315 ◽  
Author(s):  
Thomas E. Willnow
Keyword(s):  
Lipid Metabolism ◽  
Gene Family ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Multiple Roles ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Density Lipoprotein Receptor ◽  
Receptor Gene Family

Germ Cell-Somatic Cell Dichotomy of a Low-Density Lipoprotein Receptor Gene Family Member in Testis

1997 ◽  
Vol 16 (1) ◽  
pp. 35-43 ◽  
Author(s):  
KEN A. LINDSTEDT ◽  
HIDEAKI BUJO ◽  
MICHELLE G. MAHON ◽  
JOHANNES NIMPF ◽  
WOLFGANG J. SCHNEIDER
Keyword(s):  
Germ Cell ◽  
Gene Family ◽  
Somatic Cell ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Density Lipoprotein Receptor ◽  
Receptor Gene Family

scholarly journals The Low Density Lipoprotein Receptor Gene Family

1998 ◽  
Vol 273 (48) ◽  
pp. 32213-32221 ◽  
Author(s):  
Walter Stockinger ◽  
Elke Hengstschläger-Ottnad ◽  
Sabine Novak ◽  
Andrew Matus ◽  
Manfred Hüttinger ◽  
...  
Keyword(s):  
Gene Family ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Density Lipoprotein Receptor ◽  
Receptor Gene Family

scholarly journals Low Density Lipoprotein Receptor Gene Family Members Mediate Yolk Deposition

1997 ◽  
Vol 127 (5) ◽  
pp. 801S-804S ◽  
Author(s):  
Hideaki Bujo ◽  
Marcela Hermann ◽  
Ken A. Lindstedt ◽  
Johannes Nimpf ◽  
Wolfgang J. Schneider
Keyword(s):  
Gene Family ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Family Members ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Density Lipoprotein Receptor ◽  
Receptor Gene Family

Apolipoprotein A-V Interaction with Members of the Low Density Lipoprotein Receptor Gene Family†

Biochemistry ◽  
2007 ◽  
Vol 46 (12) ◽  
pp. 3896-3904 ◽  
Author(s):  
Stefan K. Nilsson ◽  
Aivar Lookene ◽  
Jennifer A. Beckstead ◽  
Jørgen Gliemann ◽  
Robert O. Ryan ◽  
...  
Keyword(s):  
Gene Family ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Apolipoprotein A ◽  
Density Lipoprotein Receptor ◽  
Receptor Gene Family

scholarly journals Endocytic trafficking of megalin/RAP complexes: dissociation of the complexes in late endosomes.

1997 ◽  
Vol 8 (3) ◽  
pp. 517-532 ◽  
Author(s):  
R P Czekay ◽  
R A Orlando ◽  
L Woodward ◽  
M Lundstrom ◽  
M G Farquhar
Keyword(s):  
Gene Family ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Late Endosomes ◽  
Early Endosomes ◽  
Density Lipoprotein Receptor ◽  
Receptor Gene Family

Megalin (gp330) is a member of the low-density lipoprotein receptor gene family. Like other members of the family, it is an endocytic receptor that binds a number of specific ligands. Megalin also binds the receptor-associated protein (RAP) that serves as an exocytic traffic chaperone and inhibits ligand binding to the receptor. To investigate the fate of megalin/RAP complexes, we bound RAP glutathione-S-transferase fusion protein (RAP-GST) to megalin at the surface of L2 yolk sac carcinoma cells and followed the trafficking of the complexes by immunofluorescence and immunogold labeling and by their distribution on Percoll gradients. We show that megalin/RAP-GST complexes, which are internalized via clathrin-coated pits, are delivered to early endosomes where they accumulate during an 18 degrees C temperature block and colocalize with transferrin and transferrin receptor. Upon release from the temperature block, the complexes travel to late endosomes where they colocalize with rab7 and can be coprecipitated with anti-RAP-GST antibodies. Dissociation of the complex occurs in late endosomes and is most likely triggered by the low pH (approximately 5.5) of this compartment. RAP is then rapidly delivered to lysosomes and degraded whereas megalin is recycled to the cell surface. When the ligand, lipoprotein lipase, was bound to megalin, the receptor was found to recycle through early endosomes. We conclude that in contrast to receptor/ligand complexes, megalin/RAP complexes traffic through late endosomes, which is a novelty for members of the low-density lipoprotein receptor gene family.

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