Evidence of alternative promoters directing isoform-specific expression of human endothelin-converting enzyme-1 mRNA in cultured endothelial cells

1997 ◽  
Vol 75 (7) ◽  
pp. 512-521 ◽  
Author(s):  
Hans-Dieter Orzechowski ◽  
Claus-Michael Richter ◽  
Heiko Funke-Kaiser ◽  
Burkhard Kröger ◽  
Martin Schmidt ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Alternative Promoters ◽  
Converting Enzyme ◽  
Specific Expression ◽  
Cultured Endothelial Cells ◽  
Endothelin Converting Enzyme

Transcriptional Mechanism of Protein Kinase C-Induced Isoform-Specific Expression of the Gene for Endothelin-Converting Enzyme-1in Human Endothelial Cells

2001 ◽  
Vol 60 (6) ◽  
pp. 1332-1342 ◽  
Author(s):  
Hans-Dieter Orzechowski ◽  
Astrid Günther ◽  
Stefan Menzel ◽  
Andreas Zimmermann ◽  
Heiko Funke-Kaiser ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Converting Enzyme ◽  
Human Endothelial Cells ◽  
Specific Expression ◽  
Kinase C ◽  
Endothelin Converting Enzyme

Expression of human endothelin-converting enzyme isoforms: role of angiotensin IIThis article is one of a selection of papers published in the special issue (part 1 of 2) on Forefronts in Endothelin.

2008 ◽  
Vol 86 (6) ◽  
pp. 299-309 ◽  
Author(s):  
W. Goettsch ◽  
A. Schubert ◽  
H. Morawietz
Keyword(s):  
Endothelial Cells ◽  
Mrna Expression ◽  
Umbilical Vein ◽  
Artery Bypass ◽  
Control Group ◽  
Ang Ii ◽  
Converting Enzyme ◽  
Mammary Arteries ◽  
Endothelin Converting Enzyme ◽  
Internal Mammary

A key step in endothelin-1 (ET-1) synthesis is the proteolytic cleavage of big ET-1 by the endothelin-converting enzyme-1 (ECE-1). Four alternatively spliced isoforms, ECE-1a to ECE-1d, have been discovered; however, regulation of the expression of specific ECE-1 isoforms is not well understood. Therefore, we stimulated primary human umbilical vein endothelial cells (HUVECs) with angiotensin II (Ang II). Furthermore, expression of ECE-1 isoforms was determined in internal mammary arteries of patients undergoing coronary artery bypass grafting surgery. Patients had received one of 4 therapies: angiotensin-converting enzyme inhibitors (ACE-I), Ang II type 1 receptor blockers (ARB), HMG-CoA reductase inhibitors (statins), and a control group that had received neither ACE-I, ARB (that is, treatment not interfering in the renin–angiotensin system), nor statins. Under control conditions, ECE-1a is the dominant isoform in HUVECs (4.5 ± 2.8 amol/μg RNA), followed by ECE-1c (2.7 ± 1.0 amol/μg), ECE-1d (0.49 ± 0.17 amol/μg), and ECE-1b (0.17 ± 0.04 amol/μg). Stimulation with Ang II did not change the ECE-1 expression pattern or the ET-1 release. We found that ECE-1 mRNA expression was higher in patients treated with statins than in patients treated with ARB therapy (5.8 ± 0.76 RU versus 3.0 ± 0.4 RU), mainly attributed to ECE-1a. In addition, ECE-1a mRNA expression was higher in patients receiving ACE-I therapy than in patients receiving ARB therapy (1.68 ± 0.27 RU versus 0.83 ± 0.07 RU). We conclude that ECE-1a is the major ECE-1 isoform in primary human endothelial cells. Its expression in internal mammary arteries can be regulated by statin therapy and differs between patients with ACE-I and ARB therapy.

Expression of the Endothelin-Converting Enzyme-1 Isoforms in Endothelial Cells

2000 ◽  
Vol 36 ◽  
pp. S15-S18
Author(s):  
Laurent Muller ◽  
Olivier Valdenaire ◽  
Alain Barret ◽  
Petra Korth ◽  
Florence Pinet ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Converting Enzyme ◽  
Endothelin Converting Enzyme

Physiologic Shear Stress Suppresses Endothelin-Converting Enzyme-1 Expression in Vascular Endothelial Cells

1998 ◽  
Vol 31 ◽  
pp. S42-S45 ◽  
Author(s):  
Ken Masatsugu ◽  
Hiroshi Itoh ◽  
Tae-Hwa Chun ◽  
Yoshihiro Ogawa ◽  
Naoshita Tamura ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Converting Enzyme ◽  
Endothelin Converting Enzyme
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