Enhanced detection of mutations in BRCA1 exon 11 using restriction endonuclease fingerprinting-single-strand conformation polymorphism

2000 ◽  
Vol 78 (10) ◽  
pp. 580-587
Author(s):  
Astanand Jugessur ◽  
Petter Frost ◽  
Tone Ikdahl Andersen ◽  
Solrun Steine ◽  
Annika Lindblom ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Exon 11 ◽  
Detection Of Mutations ◽  
Conformation Polymorphism ◽  
Brca1 Exon

scholarly journals Optimization of single-strand conformation polymorphism analysis in the presence of polyethylene glycol

1997 ◽  
Vol 43 (1) ◽  
pp. 30-33 ◽  
Author(s):  
Arseni Markoff ◽  
Alex Savov ◽  
Vladimir Vladimirov ◽  
Nadia Bogdanova ◽  
Ivo Kremensky ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gc Content ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Sscp Analysis ◽  
Polymorphism Analysis ◽  
Conformation Polymorphism Analysis ◽  
Detection Of Mutations ◽  
Conformation Polymorphism

Abstract We report optimization of single-strand conformation polymorphism (SSCP) analysis in the presence of polyethylene glycol. The protocol developed separates single-strand conformers in a much shorter time (1–3 h) than conventional SSCP protocols and broadens the applicability of SSCP analysis from 150 to as much as 500 bp of DNA by different percentages of GC content present. We conclude that addition of polyethylene glycol helps improve the differential separation of conformers and, in combination with high-resolution polyacrylamide gel electrophoresis, offers an alternative to previous SSCP analysis protocols. This protocol should be very useful for clinical applications in routine detection of mutations as well as for research purposes.

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