Effects of leukotriene B 4 receptor antagonist, LY293111Na, on antigen-induced bronchial hyperresponsiveness and leukocyte infiltration in sensitized guinea pigs

2001 ◽  
Vol 50 (3) ◽  
pp. 136-141 ◽  
Author(s):  
F. Asanuma ◽  
K. Kuwabara ◽  
A. Arimura ◽  
Y. Furue ◽  
J.H. Fleisch ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Guinea Pigs ◽  
Bronchial Hyperresponsiveness ◽  
Leukocyte Infiltration

Sulfidopeptide leukotrienes mediate acrolein-induced bronchial hyperresponsiveness

1989 ◽  
Vol 66 (4) ◽  
pp. 1838-1845 ◽  
Author(s):  
G. D. Leikauf ◽  
C. A. Doupnik ◽  
L. M. Leming ◽  
H. E. Wey
Keyword(s):  
Receptor Antagonist ◽  
Guinea Pigs ◽  
Bronchoalveolar Lavage Fluid ◽  
Bronchial Hyperresponsiveness ◽  
Lavage Fluid ◽  
Lipid Mediators ◽  
Leukotriene Receptor Antagonist ◽  
Bronchial Responsiveness ◽  
Lipoxygenase Inhibitors ◽  
Leukotriene Receptor

The sulfidopeptide leukotrienes are bronchoconstrictive lipid mediators thought to have an important role in the pathophysiology of asthma. The objective of this study was to determine if treatment with a leukotriene receptor antagonist and 5-lipoxygenase inhibitors could diminish acrolein-induced bronchial hyperresponsiveness and to determine whether leukotriene (LT) C4 generation is augmented by acrolein exposure. Guinea pigs (groups of 6–7) were exposed to 1.3 ppm acrolein for 2 h and bronchial responsiveness to intravenous acetylcholine determined twice before, and once 1, 2, 6, and 24 h after exposure. Immediately after acrolein exposure (5 min) specific total airway resistance (sRt) increased from 0.86 +/- 0.01 to 1.29 +/- 0.07 ml.cmH2O.ml-1.s. Within 1 h after exposure, the effective dose of acetylcholine sufficient to double sRt (ED200) decreased from 114.0 +/- 6.6 to 58.5 +/- 6.5 micrograms.kg-1.min-1. Bronchial hyperresponsiveness became maximal at 2 h with ED200 = 44.7 +/- 4.2 and persisted for up to 24 h after exposure (24 h ED200 = 60.2 +/- 11.6 micrograms.kg-1.min). A LTC4/LTD4 receptor antagonist, L-649,923 (10 mg/kg iv), and two putative inhibitors of 5-lipoxygenase, L-651,392 (10 mg/kg po) and U-60,257 (5 mg/kg i.v.), diminished the immediate bronchoconstriction and markedly inhibited bronchial hyperresponsiveness. Analysis of bronchoalveolar lavage fluid obtained from guinea pigs after acrolein exposure revealed a significant increase in immunoreactive LTC4 concentrations (control LTC4 = 8.8 +/- 0.3, n = 7; exposed LTC4 = 15.9 +/- 2.4 pg/ml, n = 6). Treatment with L-651,392 inhibited this response (acrolein exposed = 9.4 +/- 2.4 pg/ml, n = 5).(ABSTRACT TRUNCATED AT 250 WORDS)

A 5-HT4 agonist mosapride enhances rectorectal and rectoanal reflexes in guinea pigs

2003 ◽  
Vol 285 (2) ◽  
pp. G389-G395 ◽  
Author(s):  
Hidehiko Shimatani ◽  
Yu Kojima ◽  
Makoto Kadowaki ◽  
Tadashi Nakagawa ◽  
Hisao Fujii ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Receptor Antagonist ◽  
Strain Gauge ◽  
Guinea Pigs ◽  
Internal Anal Sphincter ◽  
Pressure Transducer ◽  
Force Transducer ◽  
Rectal Distension ◽  
Pelvic Nerves ◽  
Strain Gauge Force Transducer

The rectal distension-evoked reflex rectal (R-R) contractions and internal anal sphincter (R-IAS) relaxations in guinea pigs were generated through the extrinsic sacral excitatory nerve pathway (pelvic nerves) and the intrinsic cholinergic excitatory and nitrergic inhibitory nerve pathways. The aim of the present study was to evaluate whether a prokinetic benzamide, mosapride, enhances the R-R and R-IAS reflexes mediated via 5-HT4 receptors in the guinea pig. The mechanical activities of the R and IAS were recorded with a balloon connected to a pressure transducer and a strain gauge force transducer in the anesthetized guinea pig with intact spinal-intestinal pathways. Gradual and sustained rectal distension evoked R-R contractions and synchronous R-IAS relaxations. Mosapride (0.1–1.0 mg/kg iv) dose-dependently enhanced both R-R and R-IAS reflex responses. Reflex indexes for R-R and R-IAS maximally increased from 1.0 (control) to 1.92 and 1.88, respectively. A specific 5-HT4 receptor antagonist, GR 113808 (1.0 mg/kg iv), antagonized the enhancement of the R-R and R-IAS reflexes induced by mosapride 1.0 mg/kg iv. The present results indicate that mosapride enhanced the R-R and R-IAS reflexes mediated through 5-HT4 receptors.

scholarly journals Role of neutral endopeptidase in endotoxin-induced bronchial hyperresponsiveness to substance P in guinea pigs

1998 ◽  
Vol 47 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Shigenori Iwamae ◽  
Hideo Tsukagoshi ◽  
Takeshi Hisada ◽  
Daisuke Uno ◽  
Masatomo Mori
Keyword(s):  
Substance P ◽  
Guinea Pigs ◽  
Bronchial Hyperresponsiveness ◽  
Neutral Endopeptidase

The non-pfjptide NK-1 receptor antagonist LY303870 inhibits neurogenic dural inflammation in guinea pigs

Life Sciences ◽  
1997 ◽  
Vol 60 (18) ◽  
pp. 1553-1561 ◽  
Author(s):  
Lee A. Phebus ◽  
Kirk W. Johnson ◽  
Peter W. Stengel ◽  
Karen L. Lobb ◽  
James A. Nixon ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Guinea Pigs

scholarly journals Antithrombotic Effects of KW-3635, a Thromboxane A2-Receptor Antagonist, in Guinea Pigs.

1994 ◽  
Vol 65 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Shiro Shirakura ◽  
Katsuya Higo ◽  
Masami Takeda ◽  
Akira Karasawa
Keyword(s):  
Receptor Antagonist ◽  
Guinea Pigs ◽  
Thromboxane A2 ◽  
Thromboxane A2 Receptor ◽  
Antithrombotic Effects
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