Determination of the histamine metabolite tele-methylimidazoleacetic acid and of creatinine in urine by the same HPLC system

1999 ◽  
Vol 48 (2) ◽  
pp. 75-80 ◽  
Author(s):  
G. Granerus ◽  
B. Lönnqvist ◽  
U. Wass
Keyword(s):  
Histamine Metabolite ◽  
Hplc System ◽  
Methylimidazoleacetic Acid

Determination of acetaldehyde and acetone emanating from human skin using a passive flux sampler—HPLC system

2007 ◽  
Vol 859 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Yoshika Sekine ◽  
Satomi Toyooka ◽  
Simon F. Watts
Keyword(s):  
Human Skin ◽  
Hplc System

scholarly journals Experimental Testing of a Size-Exclusion Chromatography Method Used for Evaluation of Molecular Parameters of Equine Anti-Ebola Immunoglobulin

2019 ◽  
Vol 19 (4) ◽  
pp. 261-267
Author(s):  
Е. Yu. Mishalova ◽  
E. V. Gordeev ◽  
V. N. Lebedev ◽  
S. A. Melnikov ◽  
S. A. Nimirskaya ◽  
...  
Keyword(s):  
Specific Activity ◽  
Experimental Testing ◽  
Horse Serum ◽  
Test Procedure ◽  
Size Exclusion ◽  
Array Detector ◽  
Test Procedures ◽  
Molecular Parameters ◽  
Hplc System

Haemorrhagic fever caused by the Ebola virus is a highly hazardous infectious disease with a mortality rate of 50– 90 %. Heterologous immunoglobulins with a high virus-neutralizing titer are an important element of the WHO-endorsed set of measures for emergency prevention and treatment of the disease. Specific activity of these products is largely determined by their fractional composition, and, in particular, by molecular mass distribution (MMD). The size-exclusion-high-performance liquid chromatography (SEC-HPLC) has traditionally been used for determination of the MMD of the target protein in human immunoglobulin-based products. The use of this method for evaluation of molecular parameters of heterologous immunoglobulin requires confirmation of its specificity, accuracy and precision, and establishment of the chromatographic system suitability criteria in the context of a new test object.The aim of the study was to test the applicability of the SEC-HPLC method to the assessment of molecular parameters of anti-Ebola immunoglobulin derived from horse serum.Materials and methods: three batches of purified equine anti-Ebola immunoglobulin were used in the study. Normal equine and human immunoglobulins of the IgG isotype were used as reference standards. The HPLC test procedures described in the European Pharmacopoeia 9.6 and State Pharmacopoeia of the Russian Federation, 14th ed., were used for determination of monomers and other immunoglobulin fractions. An Agilent 1260 Infinity (Agilent, USA) HPLC system with a diode array detector and an Agilent Bio SEC-3 HPLC column were used for quality evaluation of the tested products.Results: the resolution factor between IgG monomer and dimer peaks (1.69 and 2.10), and the chromatographic column efficiency (>2000) make it possible to use the SEC-HPLC system for evaluation of molecular parameters of heterologous immunoglobulin. The study demonstrated reproducibility of the test procedure.Conclusions: the study confirmed the applicability of the SEC-HPLC procedure for evaluation of molecular parameters of anti-Ebola immunoglobulin derived from horse serum. It demonstrated the compliance of the purified immunoglobulin to the national and international quality requirements in terms of «Molecular parameters».

A common method for the determination of several calcium channel blockers using an HPLC system with ultraviolet detection

Talanta ◽  
1998 ◽  
Vol 47 (5) ◽  
pp. 1245-1254 ◽  
Author(s):  
M.A Garcı́a ◽  
C Soláns ◽  
J.J Aramayona ◽  
L.J Fraile ◽  
M.A Bregante ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Calcium Channel Blockers ◽  
Common Method ◽  
Channel Blockers ◽  
Ultraviolet Detection ◽  
Hplc System

Total and individual determination of carbamate pesticides by use of an integrated flow-injection/HPLC system

1992 ◽  
Vol 33 (9-10) ◽  
pp. 449-453 ◽  
Author(s):  
M. T. Tena ◽  
P. Linares ◽  
M. D. Luque de Castro ◽  
M. Valcárcel
Keyword(s):  
Flow Injection ◽  
Carbamate Pesticides ◽  
Hplc System ◽  
Individual Determination
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