Effects of prostanoid receptor agonists on immunologically-activated rat peritoneal mast cells

1998 ◽  
Vol 47 (0) ◽  
pp. 20-21 ◽  
Author(s):  
C. L. Chan ◽  
H. Y. A. Lau
Keyword(s):  
Mast Cells ◽  
Prostanoid Receptor ◽  
Receptor Agonists ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

scholarly journals α1-Adrenergic Receptor Blockade by Prazosin Synergistically Stabilizes Rat Peritoneal Mast Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Nozomu Abe ◽  
Hiroaki Toyama ◽  
Yutaka Ejima ◽  
Kazutomo Saito ◽  
Tsutomu Tamada ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Receptor Antagonist ◽  
Adrenergic Receptors ◽  
Adrenergic Receptor ◽  
High Dose ◽  
Patch Clamp Technique ◽  
Receptor Agonists ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

Background. Adrenaline quickly inhibits the release of histamine from mast cells. Besides β2-adrenergic receptors, several in vitro studies also indicate the involvement of α-adrenergic receptors in the process of exocytosis. Since exocytosis in mast cells can be detected electrophysiologically by the changes in the membrane capacitance (Cm), its continuous monitoring in the presence of drugs would determine their mast cell-stabilizing properties. Methods. Employing the whole-cell patch-clamp technique in rat peritoneal mast cells, we examined the effects of adrenaline on the degranulation of mast cells and the increase in the Cm during exocytosis. We also examined the degranulation of mast cells in the presence or absence of α-adrenergic receptor agonists or antagonists. Results. Adrenaline dose-dependently suppressed the GTP-γ-S-induced increase in the Cm and inhibited the degranulation from mast cells, which was almost completely erased in the presence of butoxamine, a β2-adrenergic receptor antagonist. Among α-adrenergic receptor agonists or antagonists, high-dose prazosin, a selective α1-adrenergic receptor antagonist, significantly reduced the ratio of degranulating mast cells and suppressed the increase in the Cm. Additionally, prazosin augmented the inhibitory effects of adrenaline on the degranulation of mast cells. Conclusions. This study provided electrophysiological evidence for the first time that adrenaline dose-dependently inhibited the process of exocytosis, confirming its usefulness as a potent mast cell stabilizer. The pharmacological blockade of α1-adrenergic receptor by prazosin synergistically potentiated such mast cell-stabilizing property of adrenaline, which is primarily mediated by β2-adrenergic receptors.

scholarly journals Native heparin from rat peritoneal mast cells.

1977 ◽  
Vol 252 (2) ◽  
pp. 518-521 ◽  
Author(s):  
R W Yurt ◽  
R W Leid ◽  
K F Austen
Keyword(s):  
Mast Cells ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

The Potencies of Thapsigargin and Analogues as Activators of Rat Peritoneal Mast Cells

Planta Medica ◽  
1986 ◽  
Vol 52 (04) ◽  
pp. 251-255 ◽  
Author(s):  
Elsebeth Norup ◽  
Ulla Smitt ◽  
S. Christensen
Keyword(s):  
Mast Cells ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells
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