Early Identification of Mycobacterium tuberculosis and Mycobacterium avium Using the Polymerase Chain Reaction on Samples Positive by a Rapid Commercial Culture System

1999 ◽  
Vol 18 (5) ◽  
pp. 346-351 ◽  
Author(s):  
C. Sion ◽  
J. Degraux ◽  
M. Delmée
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Avium ◽  
Early Identification ◽  
Culture System ◽  
Commercial Culture ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals ASSESSMENT OF DIAGNOSTIC TECHNIQUES OF URINARY TUBERCULOSIS

2013 ◽  
Vol 5 (1) ◽  
pp. e2013034 ◽  
Author(s):  
Khaled Ismail Ghaleb ◽  
Magdy Mohamed Afifi ◽  
Mohamed Mohamed El Gohary
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Early Diagnosis ◽  
Culture System ◽  
Chain Reaction ◽  
Lowenstein Jensen ◽  
Polymerase Chain ◽  
Urinary Tuberculosis ◽  
Dna Enzyme Immunoassay ◽  
E Mail

    Khalid Ghaleb a,* , Magdy Afifib, Mohamad El-Gohary c aDepartment of Medical Laboratories, Faculty of Applied Medical Science, King Khalid University, Bisha 551, Saudia Arabia bDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assuit 71524, Egypt cDepartment of Internal Medicine, Faculty of Medicine, Al-Azhar University, Assuit, Egypt The corresponding author e-mail: [email protected] Current Tel: 00966595388496 Saudia,  00201119338055 Egypt The place of the study worked : Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assuit 71524, Egypt, e-mail: [email protected]  Tel: 00201006554961 Abstract Early diagnosis of active tuberculosis remains an elusive challenge. In addition, one third of the world's population is latently infected with Mycobacterium tuberculosis (Mtb) and up to 10% of infected individuals develop tuberculosis (TB) in their lifetime. In this investigation, the incidence of urinary tuberculosis among renal patients was studied. Three hundreds urine samples were processed for detection of Mtb by Ziehl-Neelson (ZN) smear examination, Lowenstein Jensen (LJ) medium, radiometric BACTEC460 system as well as polymerase chain reaction (PCR) and DNA Enzyme Immunoassay (DEIA) test.  Out of 300 urine samples, 2 were positive by both  ZN smears and LJ medium with incidence rate of 0.66 %, 3 positive samples by BACTEC460 culture system with incidence of 1%. PCR assay gave more positive results than smear and culture examination (i.e. 8 positive samples with incidence  rate of 2.6%).  The specificities were 25% for both ZN smears and LJ medium, 37.5% for BACTEC460 culture system, and 100% for PCR test, while  sensitivities of all assays were 100%. Thus PCR is a rapid and sensitive method for the early diagnosis of urinary tuberculosis.   Keywords: List of abbreviations:Acid Fast Bacilli (AFB)-Base pair (bp)-DNA Enzyme Immunoassay (DEIA)  -Extrapulmonary Tuberculosis (EPTB)-International Union Against Tuberculosis  (IUAT)-Lowenstein Jensen (LJ)-Mycobacterium tuberculosis (Mtb) -Polymerase Chain Reaction (PCR)-Tuberculosis (TB)-Urinary Tuberculosis (UTB-Urogenital Tuberculosis (UGTB)-Ziehl-Neelson (ZN)  

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