Cloning and Characterization of a Polygalacturonase Gene Ap2pg1 from Geotrichum candidum Citrus Race Ap2 Pathogenic to Apple Fruit

2002 ◽  
Vol 68 (4) ◽  
pp. 333-337 ◽  
Author(s):  
Masayuki NAKAMURA ◽  
Hisashi IWAI ◽  
Kei ARAI
Keyword(s):  
Geotrichum Candidum ◽  
Apple Fruit ◽  
Geotrichum Candidum Citrus Race ◽  
Polygalacturonase Gene

scholarly journals Quantitative Characterization of Geotrichum candidum Growth in Milk

2021 ◽  
Vol 11 (10) ◽  
pp. 4619
Author(s):  
Petra Šipošová ◽  
Martina Koňuchová ◽  
Ľubomír Valík ◽  
Monika Trebichavská ◽  
Alžbeta Medveďová
Keyword(s):  
Growth Parameters ◽  
Mechanistic Model ◽  
Geotrichum Candidum ◽  
Activity Level ◽  
Effect Of Temperature ◽  
Operational Parameters ◽  
Quantitative Characterization ◽  
Essential Knowledge ◽  
Cardinal Temperatures

The study of microbial growth in relation to food environments provides essential knowledge for food quality control. With respect to its significance in the dairy industry, the growth of Geotrichum candidum isolate J in milk without and with 1% NaCl was investigated under isothermal conditions ranging from 6 to 37 °C. The mechanistic model by Baranyi and Roberts was used to fit the fungal counts over time and to estimate the growth parameters of the isolate. The effect of temperature on the growth of G. candidum in milk was modelled with the cardinal models, and the cardinal temperatures were calculated as Tmin = −3.8–0.0 °C, Topt = 28.0–34.6 °C, and Tmax = 35.2–37.2 °C. The growth of G. candidum J was slightly faster in milk with 1% NaCl and in temperature regions under 21 °C. However, in a temperature range that was close to the optimum, its growth was slightly inhibited by the lowered water activity level. The present study provides useful cultivation data for understanding the behaviour of G. candidum in milk and can serve as an effective tool for assessing the risk of fungal spoilage, predicting the shelf life of dairy products, or assessing the optimal conditions for its growth in relation to the operational parameters in dairy practices.

scholarly journals Characterization of Postharvest Fungicide-Resistant Botrytis cinerea Isolates From Commercially Stored Apple Fruit

2017 ◽  
Vol 107 (3) ◽  
pp. 362-368 ◽  
Author(s):  
Wayne M. Jurick ◽  
Otilia Macarisin ◽  
Verneta L. Gaskins ◽  
Eunhee Park ◽  
Jiujiang Yu ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Apple Fruit ◽  
Sublethal Dose ◽  
Long Term Storage ◽  
Pose Control ◽  
First Time

Botrytis cinerea causes gray mold and is an economically important postharvest pathogen of fruit, vegetables, and ornamentals. Fludioxonil-sensitive B. cinerea isolates were collected in 2011 and 2013 from commercial storage in Pennsylvania. Eight isolates had values for effective concentrations for inhibiting 50% of mycelial growth of 0.0004 to 0.0038 μg/ml for fludioxonil and were dual resistant to pyrimethanil and thiabendazole. Resistance was generated in vitro, following exposure to a sublethal dose of fludioxonil, in seven of eight dual-resistant B. cinerea isolates. Three vigorously growing B. cinerea isolates with multiresistance to postharvest fungicides were further characterized and found to be osmosensitive and retained resistance in the absence of selection pressure. A representative multiresistant B. cinerea strain caused decay on apple fruit treated with postharvest fungicides, which confirmed the in vitro results. The R632I mutation in the Mrr1 gene, associated with fludioxonil resistance in B. cinerea, was not detected in multipostharvest fungicide-resistant B. cinerea isolates, suggesting that the fungus may be using additional mechanisms to mediate resistance. Results from this study show for the first time that B. cinerea with dual resistance to pyrimethanil and thiabendazole can also rapidly develop resistance to fludioxonil, which may pose control challenges in the packinghouse environment and during long-term storage.

scholarly journals Characterization of the Bull's Eye Rot of Apple in Chile

Plant Disease ◽  
2013 ◽  
Vol 97 (4) ◽  
pp. 485-490 ◽  
Author(s):  
Sylvana Soto-Alvear ◽  
Mauricio Lolas ◽  
Inés M. Rosales ◽  
Eduardo R. Chávez ◽  
Bernardo A. Latorre
Keyword(s):  
Morphological Characteristics ◽  
The United States ◽  
Apple Fruit ◽  
Maritime Transportation ◽  
Tubulin Genes ◽  
Internal Transcribed Spacer Sequences ◽  
Apple Production ◽  
Species Specific ◽  
Bull's Eye

Apple fruit in Chile are primarily produced for export to Asia, Europe, and the United States, which typically requires 15 to 40 days of maritime transportation. Therefore, Chilean apple production must fulfill the sanitization requirements imposed by the receiving countries. Under these circumstances, it was important to clarify the etiology of bull's eye rot that can severely affect ‘Cripps Pink’ apple and other late-harvest cultivars in Chile. Based on morphological characteristics and the partial sequence analysis of the internal transcribed spacer sequences and β-tubulin genes, Neofabraea alba was identified as the causal agent of the bull's eye rot of Chilean apple. These results were further corroborated using species-specific primers. The incidence of bull's eye rot varied considerably; for instance, in 2009, 0.0 to 58.7% in 38 Cripps Pink orchards surveyed in the relatively arid and humid apple-growing areas of Chile, respectively. There was no evidence for the presence of N. malicorticis or N. perennans, which are commonly identified as causal agents of bull's eye rot in other apple-producing countries. Altogether, these data suggest that N. alba might represent the predominant and possibly the only cause of bull's-eye rot of Chilean apple.

Separation and Characterization of Two Molecular Forms of Geotrichum candidum Lipase

1990 ◽  
Vol 107 (3) ◽  
pp. 426-430 ◽  
Author(s):  
Akio Sugihara ◽  
Yuji Shimada ◽  
Yoshio Tominaga
Keyword(s):  
Geotrichum Candidum ◽  
Molecular Forms

Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin

2010 ◽  
Vol 56 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Simona Marianna Sanzani ◽  
Leonardo Schena ◽  
Annalisa De Girolamo ◽  
Antonio Ippolito ◽  
Luis González-Candelas
Keyword(s):  
Induced Resistance ◽  
Apple Fruit ◽  
Penicillium Expansum

Characterization of laccase from apple fruit during postharvest storage and its response to diphenylamine and 1-methylcyclopropene treatments

2018 ◽  
Vol 253 ◽  
pp. 314-321 ◽  
Author(s):  
Gong Yihui ◽  
Jun Song ◽  
Lina Du ◽  
Mindy Vinqvist ◽  
Leslie Campbell Palmer ◽  
...  
Keyword(s):  
Apple Fruit ◽  
Postharvest Storage

Isolation and Characterization of Pectin Methylesterase from Apple Fruit

1989 ◽  
Vol 54 (3) ◽  
pp. 653-655 ◽  
Author(s):  
D. CASTALDO ◽  
L. QUAGLIUOLO ◽  
L. SERVILLO ◽  
C. BALESTRIERI ◽  
A. GIOVANE
Keyword(s):  
Pectin Methylesterase ◽  
Apple Fruit ◽  
Isolation And Characterization

Production, partial purification, and immunochemical characterization of multiple forms of lipase from Geotrichum candidum

1989 ◽  
Vol 11 (2) ◽  
pp. 90-95 ◽  
Author(s):  
Tomas Jacobsen ◽  
Jorgen Olsen ◽  
Knud Allermann ◽  
Otto Melcior Poulsen ◽  
Jann Hau
Keyword(s):  
Geotrichum Candidum ◽  
Partial Purification ◽  
Multiple Forms ◽  
Immunochemical Characterization
Sign in / Sign up

Export Citation Format

Share Document