Cell separation by expanded bed adsorption: use of ion exchange chromatography for the separation of E. coli and S. cerevisiae

2000 ◽  
Vol 23 (3) ◽  
pp. 245-250 ◽  
Author(s):  
L. B. Ujam ◽  
R. H. Clemmitt ◽  
H. A. Chase
Keyword(s):  
Ion Exchange ◽  
Cell Separation ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
E Coli

scholarly journals A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery

2009 ◽  
Vol 52 (2) ◽  
pp. 427-436 ◽  
Author(s):  
João Batista Severo Jr. ◽  
Roberto Rodrigues de Souza ◽  
José Carlos Curvelo Santana ◽  
Elias Basile Tambourgi
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Binding Capacity ◽  
Ion Exchange Resin ◽  
Exchange Chromatography ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Bed Height ◽  
Salt Addition ◽  
Bed Expansion

In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.

Recovery of Nuclease Produced by Lactococcus Lactis Using Expanded Bed Ion Exchange Chromatography

2006 ◽  
Vol 28 (13) ◽  
pp. 1033-1039 ◽  
Author(s):  
E. Bodo ◽  
A. Durieux ◽  
C. Saint-Hubert ◽  
R. Lavallée ◽  
J-M Boufflette ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Lactococcus Lactis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Expanded Bed

scholarly journals Purification of Peroxidase from Rice Bran Using Expanded-Bed Ion-Exchange Chromatography

2015 ◽  
Vol 33 (2) ◽  
pp. 153-164 ◽  
Author(s):  
G.V. Gautério ◽  
S.S. Fernandes ◽  
F.O. Molon ◽  
F.S. Figueira ◽  
J.G. Buffon ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Rice Bran ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Expanded Bed

scholarly journals Characterisation of Lactoferrin Isolated from Acid Whey Using Pilot-Scale Monolithic Ion-Exchange Chromatography

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 804
Author(s):  
Bojana Bogovič Matijašić ◽  
Jernej Oberčkal ◽  
Petra Mohar Lorbeg ◽  
Diana Paveljšek ◽  
Nina Skale ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ion Exchange ◽  
Binding Capacity ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Iron Binding ◽  
E Coli ◽  
Freeze Dried ◽  
Acid Whey ◽  
Iron Binding Capacity

The aim of this study was to characterize the properties of lactoferrin (LF) obtained in a process developed for its isolation from acid whey derived from the production of fresh curd cheese, using a unique technology of ion-exchange chromatography on CIM® monolithic columns. The freeze-dried lactoferrin samples produced on the pilot plant (capacity 1 m3) were examined for the purity, iron-binding capacity, antibacterial activity, and pH- and temperature-stability. Apo-LF inhibited several tested strains (enterobacteria, Staphylococcus, Streptococcus salivarius) except clostridia, lactic acid bacteria, and bifidobacteria. Sample of LF intentionally saturated with Fe3+ lost its antibacterial activity, indicating the involvement of mechanisms based on depriving bacteria of an iron source. All samples, regardless of the iron-saturation level, exhibited stability in pH range 4.0 to 11.0. LF with higher iron content (A-value = 41.9%) showed better thermal stability. Heat treatment up to 72 °C/3 s did not reduce antimicrobial activity against E. coli O157: H7 tox-. Higher purity (above 91%), higher iron-binding capacity and higher inhibitory activity against E. coli O157: H7 tox- compared to some similar products from the market was observed. These results demonstrate a high potential of monolithic ion-exchange chromatography for industrial processing of acid whey as a source of LF that can be used in new products with high-added value. The upscaling of the process is ongoing on a demonstration plant (10–30 m3/day capacity).

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