Optimization of medium components for phytase production by E. coli using response surface methodology

1999 ◽  
Vol 21 (6) ◽  
pp. 477-481 ◽  
Author(s):  
K. Sunitha ◽  
Jung-Kee Lee ◽  
Tae-Kwang Oh
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Phytase Production ◽  
E Coli ◽  
Medium Components

scholarly journals Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

2017 ◽  
Vol 26 (1) ◽  
pp. 57-64
Author(s):  
Abd-El Aziem Farouk ◽  
Anis Shobirin Meor Hussin ◽  
Ralf Greiner ◽  
Shareef Mohideen Ismail ◽  
Hamadah Mohd Nur Lubis
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Temperature ◽  
Expression System ◽  
Optimal Conditions ◽  
Phytase Production ◽  
E Coli ◽  
Central Composite Experimental Design ◽  
Full Factorial ◽  
Central Composite

The production of phytase in a recombinant E.coli using the pBAD expression  system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3  central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature.  Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.   

Optimisation of Culture media through Response surface methodology to improve Antioxidant activity of E. coli

2021 ◽  
pp. 4909-4912
Author(s):  
Neha Sharma ◽  
Sharmistha Banerjee ◽  
Shuchi Kaushik ◽  
Rajesh Singh Tomar
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Culture Media ◽  
Antioxidant Property ◽  
Scavenging Activity ◽  
E Coli ◽  
Medium Components ◽  
Extracellular Metabolites ◽  
Wet Biomass

Antioxidants play a significant role in oxidative stress management and health protection. One variable at a time response surface methodology (RSM) was used to formulate different composition of media to increase the antioxidant property of selected microorganism (Escherichia coli MTCC no. 40). 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assays was used to measure antioxidant activity of selected microorganism. The reduction potentiality of DPPH radical was determined by the DPPH% antioxidant or scavenging activity of the solution. In the present study, we have measured antioxidant activity of both intracellular and extracellular metabolites in dry and wet extracellular metabolites in supernatant respectively. In DPPH assay, extracellular metabolites showed better antioxidant potential in comparison with the reference compound. The comparison was based on antioxidant activity in different responses (number 1-16) for both dry and wet biomass. According to the results maximum DPPH % antioxidant or scavenging activity was showed by response 9 (wet biomass) and response 6 (dry biomass) that is 24.8447 and 35.0142 respectively in comparison to standard (4.4636). Hence, response surface methodology is used in bioprocess technology to optimize the medium components which is the primary step involved to enhance the antioxidant activity of particular microorganism.

scholarly journals R unning Head: Novel Recombinant Phytate - d egrading Enzyme Production Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

2017 ◽  
Vol 26 (1) ◽  
Author(s):  
Abd - ElAziem Farouk ◽  
Anis Shobirin Meor Hussin ◽  
Ralf Greiner ◽  
Shareef Mohideen Ismail ◽  
Mohamed Faizal Batcha ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Temperature ◽  
Expression System ◽  
Phytase Production ◽  
E Coli ◽  
Significant Parameter ◽  
Central Composite Experimental Design ◽  
Full Factorial ◽  
Central Composite

The production of phytase in a recombinant E.coliusing thepBAD expression system wasoptimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentrationin the cultivation media and the incubation temperature were optimized in order to maximize phytase productionusing 23 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among thesefactors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. OPtim al conditionsfor phytase production were determinedto be100 μg/ml ampicilin, 0.2 % arabinose and an incubation temperature of37ºC.The production of phytase in the recombinant E. coliwas scaledup to 100ml and 1000ml.

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