A semi-structured model for the growth and β-Galactosidase production by fed-batch fermentation of Kluyveromyces marxianus

1999 ◽  
Vol 21 (4) ◽  
pp. 313-318
Author(s):  
N. Bojorge ◽  
B. Valdman ◽  
F. Acevedo ◽  
J. C. Gentina
2013 ◽  
Vol 2 (3) ◽  
pp. 127-131 ◽  
Author(s):  
H Hadiyanto ◽  
D. Ariyanti ◽  
A.P. Aini ◽  
D.S. Pinundi

Nowadays reserve of fossil fuel has gradually depleted. This condition forces many researchers to  find energy alternatives which is renewable and sustainable in the future. Ethanol derived from cheese industrial waste (whey) using fermentation process can be a new perspective in order to secure both energy and environment. The aim of this study was  to compare the operation modes (batch and fed-batch) of fermentation system on ethanol production from whey using Kluyveromyces marxianus. The result showed that the fermentation process for ethanol production by fed-batch system was higher at some point of parameters compared with batch system. Growth rate and ethanol yield (YP/S) of fed-batch fermentation were 0.122/h and 0.21 gP/gS respectively; growth rate and ethanol yield (YP/S) of batch fermentation were 0.107/h, and 0.12 g ethanol/g substrate, respectively. Based on the data of biomass and ethanol concentrations, the fermentation process for ethanol production by fed-batch system were higher at some point of parameters compared to batch system. Periodic substrate addition performed on fed-batch system leads the yeast growth in low substrate concentrations and consequently  increasing their activity and ethanol productivity. Keywords: batch; ethanol; fed-batch; fermentation;Kluyveromyces marxianus, whey


1999 ◽  
Vol 21 (4) ◽  
pp. 313 ◽  
Author(s):  
N. Bojorge ◽  
B. Valdman ◽  
F. Acevedo ◽  
J. C. Gentina

2008 ◽  
Vol 4 (1) ◽  
Author(s):  
Marcia Luciana Cazetta ◽  
Rubens Monti ◽  
Jonas Contiero

The effects of sucrose concentration, pH, temperature and aeration rate on the production of biomass and inulinase (2,1-beta-D-fructan fructanohydrolase, EC 3.2.1.7) were investigated using fed-batch fermentation by Kluyveromyces marxianus var. bulgaricus. The best sucrose concentration for enzyme activity was 10 g L-1, whereas activity was inhibited when the sucrose concentration was 20 g L-1. Enzyme activity was strongly influenced by pH of the culture. pH 5.0 was the most suitable for both enzyme activity and cell growth, achieving 98.04 U mL-1 and 14.05 g L-1, respectively, after 72 hours of cultivation. The highest production of inulinase and biomass was observed at temperatures of 25oC and 30oC, whereas a decrease in activity was observed at 35oC and 40oC. The best aeration rate was 1.0 vvm. The increase in aeration rate or oxygen injection provided no increase in enzyme activity and biomass.


2014 ◽  
Vol 47 ◽  
pp. 108-112 ◽  
Author(s):  
Hadiyanto ◽  
Dessy Ariyanti ◽  
Apsari Puspita Aini ◽  
Desiyantri Siti Pinundi

2012 ◽  
Vol 18 (5) ◽  
pp. 791
Author(s):  
Xiaoyun DING ◽  
Bin ZHUGE ◽  
Huiying FANG ◽  
Hong ZONG ◽  
Xiaoxiao LIU ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document