Kinetics of phenol degradation using Pseudomonas putida MTCC 1194

1998 ◽  
Vol 18 (5) ◽  
pp. 373-377 ◽  
Author(s):  
K. Bandyopadhyay ◽  
D. Das ◽  
B. R. Maiti
Keyword(s):  
Pseudomonas Putida ◽  
Phenol Degradation ◽  
Kinetics Of

scholarly journals Kinetics of Cometabolic Transformation of 4-chlorophenol and Phenol Degradation by Pseudomonas putida Cells in Batch and Biofilm Reactors

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1663
Author(s):  
Yen-Hui Lin
Keyword(s):  
Pseudomonas Putida ◽  
Specific Growth ◽  
Competitive Inhibition ◽  
Phenol Degradation ◽  
Inhibition Constant ◽  
Utilization Rate ◽  
Transformation Rate ◽  
Biofilm Reactors ◽  
Half Saturation Constant ◽  
Kinetics Of

The biodegradation kinetics of 4-chlorophenol (4-CP) and phenol and microbial growth of Pseudomonas putida (P. putida) cells were estimated in batch and biofilm reactors. The kinetic parameters of cells on phenol were determined using the Haldane formula. The maximum specific growth rate of P. putida on phenol, the half-saturation constant of phenol and the self-inhibition constant of phenol were 0.512 h−1, 78.38 mg/L and 228.5 mg/L, respectively. The yield growth of cells on phenol (YP) was 0.618 mg phenol/mg cell. The batch experimental results for the specific transformation rate of 4-CP by resting P. putida cells were fitted with Haldane kinetics to evaluate the maximum specific utilization rate of 4-CP, half-saturation constant of 4-CP, and self-inhibition constant of 4-CP, which were 0.246 h−1, 1.048 mg/L and 53.40 mg/L, respectively. The negative specific growth rates of cells on 4-CP obtained were fitted using a kinetic equation to investigate the true transformation capacity and first-order endogenous decay coefficient, which were 4.34 mg 4-CP/mg cell and 5.99 × 10−3 h−1, respectively. The competitive inhibition coefficients of phenol to 4-CP transformation and 4-CP to phenol degradation were 6.75 and 9.27 mg/L, respectively; therefore, phenol had a higher competitive inhibition of 4-CP transformation than the converse. The predicted model examining cometabolic transformation of 4-CP and phenol degradation showed good agreement with the experimental observations. The removal efficiencies for phenol and 4-CP were 94.56–98.45% and 96.09–98.85%, respectively, for steady-state performance.

Kinetics of phenol degradation using

1998 ◽  
Vol 18 (5) ◽  
pp. 373 ◽  
Author(s):  
K. Bandyopadhyay ◽  
D. Das ◽  
B. R. Maiti
Keyword(s):  
Phenol Degradation ◽  
Kinetics Of

Transcriptional kinetics of the cross-talk between the ortho -cleavage and TOL pathways of toluene biodegradation in Pseudomonas putida mt-2

2016 ◽  
Vol 228 ◽  
pp. 112-123 ◽  
Author(s):  
Argyro Tsipa ◽  
Michalis Koutinas ◽  
Efstratios N. Pistikopoulos ◽  
Athanasios Mantalaris
Keyword(s):  
Pseudomonas Putida ◽  
Cross Talk ◽  
The Cross ◽  
Kinetics Of ◽  
Toluene Biodegradation

scholarly journals Different Spectra of Stationary-Phase Mutations in Early-Arising versus Late-Arising Mutants of Pseudomonas putida: Involvement of the DNA Repair Enzyme MutY and the Stationary-Phase Sigma Factor RpoS

2002 ◽  
Vol 184 (24) ◽  
pp. 6957-6965 ◽  
Author(s):  
Signe Saumaa ◽  
Andres Tover ◽  
Lagle Kasak ◽  
Maia Kivisaar
Keyword(s):  
Stationary Phase ◽  
Pseudomonas Putida ◽  
Transcriptional Activation ◽  
Sigma Factor ◽  
Phenol Degradation ◽  
Test System ◽  
Growth Barriers ◽  
Mutational Processes ◽  
Sigma Factor Rpos ◽  
Selection Of

ABSTRACT Stationary-phase mutations occur in populations of stressed, nongrowing, and slowly growing cells and allow mutant bacteria to overcome growth barriers. Mutational processes in starving cells are different from those occurring in growing bacteria. Here, we present evidence that changes in mutational processes also take place during starvation of bacteria. Our test system for selection of mutants based on creation of functional promoters for the transcriptional activation of the phenol degradation genes pheBA in starving Pseudomonas putida enables us to study base substitutions (C-to-A or G-to-T transversions), deletions, and insertions. We observed changes in the spectrum of promoter-creating mutations during prolonged starvation of Pseudomonas putida on phenol minimal plates. One particular C-to-A transversion was the prevailing mutation in starving cells. However, with increasing time of starvation, the importance of this mutation decreased but the percentage of other types of mutations, such as 2- to 3-bp deletions, increased. The rate of transversions was markedly elevated in the P. putida MutY-defective strain. The occurrence of 2- to 3-bp deletions required the stationary-phase sigma factor RpoS, which indicates that some mutagenic pathway is positively controlled by RpoS in P. putida.

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