Modelling of the L-glutamic acid production with Corynebacterium glutamicum under biotin limitation

1997 ◽  
Vol 17 (3) ◽  
pp. 139-142 ◽  
Author(s):  
R. Bona ◽  
A. Moser
Keyword(s):  
Glutamic Acid ◽  
Corynebacterium Glutamicum ◽  
Acid Production ◽  
Glutamic Acid Production ◽  
Biotin Limitation

scholarly journals High production of L-glutamic acid from date juice extracts by Corynebacterium glutamicum using fed-batch cultures: pulsed and continuous feeding modes

2021 ◽  
Vol 8 (1) ◽  
pp. 37-50
Author(s):  
Mouffok Abdenacer ◽  
Nancib Aicha ◽  
Boudrant Joseph ◽  
Nancib Nabil
Keyword(s):  
Glutamic Acid ◽  
Corynebacterium Glutamicum ◽  
Ammonium Sulfate ◽  
Batch Culture ◽  
Acid Production ◽  
Fed Batch ◽  
Feeding Modes ◽  
Continuous Feeding ◽  
Date Juice ◽  
Glutamic Acid Production

Abstract In the present work, L-glutamic acid production by Corynebacterium glutamicum fermentation on date juice extracts applying two fed-batch feeding modes, pulsed and continuous, were investigated. According to the obtained results, the continuous feeding fed-batch mode was found to be the most efficient process. Moreover the continuous feeding rate mode with a feeding medium containing date juice sugars enriched with ammonium sulfate was found even more favorable as it enhances the L-glutamic acid production by approximately 2.35 fold more than the batch culture and by about 1.17 fold more than the pulsed feeding. In this respect, comparing the traditional batch culture to the continuously fed culture with a medium containing date juice sugars with ammonium sulfate showed increases of 135.47% in L-glutamic acid production, 104% in productivity, 39.09% in biomass, and 47.69% in the yield respectively allowing us to reach a final L-glutamic acid concentration of about 138 g/L, the highest ever published.

Enhanced L-glutamic acid production by immobilized Corynebacterium glutamicum X680 using Response Surface Methodology

2021 ◽  
Vol 16 (12) ◽  
pp. 87-94
Author(s):  
Subhadeep Ganguly ◽  
Smaranika Pattnaik
Keyword(s):  
Response Surface Methodology ◽  
Glutamic Acid ◽  
Response Surface ◽  
Corynebacterium Glutamicum ◽  
Acid Production ◽  
Immobilized Cells ◽  
Lower Amount ◽  
Acid Fermentation ◽  
Statistical Tool ◽  
Glutamic Acid Production

L-glutamic acid is a non-essential amino acid largely used as flavor enhancer and food additive. It also has several therapeutic applications. Fermentation has gained superiority over its chemical synthesis as it produced stereo-specific isomer. Corynebacterium glutamicum is mostly used microorganism for Lglutamic acid fermentation. The study was dealing with optimization of L-glutamic acid production by immobilized mutant Corynebacterium glutamicum X680 in calcium alginate beads using response surface methodology as effective statistical tool. Among several parameters studied, pH, inoculums size, incubation time, concentration of sodium alginate, agitation and cell:alginate ration showed the most significant effect. Immobilized cells produced significantly (p<0.01) lower amount of L-glutamic acid (24.3mg/ml) compared to the production by free cells (27.6mg/ml). However, reusability of the beads minimized production cost and hence conferred benefit as far as the market economy is concerned.

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