Taxonomic position of the human intestinal protozoan parasite Isospora belli as based on ribosomal RNA sequences

2000 ◽  
Vol 86 (8) ◽  
pp. 669-676 ◽  
Author(s):  
C. Franzen ◽  
A. Müller ◽  
R. Bialek ◽  
V. Diehl ◽  
B. Salzberger ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Protozoan Parasite ◽  
Taxonomic Position ◽  
Intestinal Protozoan ◽  
Rna Sequences ◽  
Ribosomal Rna Sequences

Characterization of the first European isolate ofNeospora caninum(Dubey, Carpenter, Speer, Topper and Uggla)

Parasitology ◽  
1995 ◽  
Vol 111 (5) ◽  
pp. 563-568 ◽  
Author(s):  
J. S. Barber ◽  
O. J. M. Holmdahl ◽  
M. R. Owen ◽  
F. Guy ◽  
A. Uggla ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Neospora Caninum ◽  
Severe Disease ◽  
Clinical Signs ◽  
Protozoan Parasite ◽  
The United States ◽  
Rna Sequences ◽  
European Isolate ◽  
Tissue Sections ◽  
Ribosomal Rna Sequences

SUMMARYNeospora caninumis an apicomplexan, protozoan parasite, which causes severe disease in dogs and cattle. It has previously been isolated only in the United States. A 5-week-old Boxer pup with a progressive hindlimb paresis was diagnosed as suffering from neosporosis on the basis of clinical signs and the presence of anti-Neosporaantibodies in it, 2 litter-mates and its darn. Despite treatment with sulphonamides, the pup was euthanased 3 days later. The diagnosis of neosporosis was confirmed by immunohistochemical examination of muscle and CNS tissue sections from the pup. Parasites were isolated into Vero cell culture from the cerebrum, and confirmed asNeospora caninumby immunofluorescence with specific antibody, tachyzoite ultrastructure and 16S-like ribosomal RNA sequences. This isolate (designated NC-Liverpool) has been continuously passaged every 7–10 days. Its growth characteristics, ultrastructure and antigenic profile, as revealed by immunoblotting, have revealed no major differences from the American NC-1 isolate. Furthermore, no difference was seen when comparing the sequences of 16S-like ribosomal RNA and the ITS1 region of the two isolates.

scholarly journals High Diversity of Cryptosporidium Species and Subtypes Identified in Cryptosporidiosis Acquired in Sweden and Abroad

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 523
Author(s):  
Marianne Lebbad ◽  
Jadwiga Winiecka-Krusnell ◽  
Christen Rune Stensvold ◽  
Jessica Beser
Keyword(s):  
Cryptosporidium Parvum ◽  
Ribosomal Rna ◽  
Diarrheal Disease ◽  
Protozoan Parasite ◽  
Small Subunit ◽  
Cryptosporidium Species ◽  
Intestinal Protozoan ◽  
Genotype I ◽  
Glycoprotein Gene ◽  
High Diversity

The intestinal protozoan parasite Cryptosporidium is an important cause of diarrheal disease worldwide. The aim of this study was to expand the knowledge on the molecular epidemiology of human cryptosporidiosis in Sweden to better understand transmission patterns and potential zoonotic sources. Cryptosporidium-positive fecal samples were collected between January 2013 and December 2014 from 12 regional clinical microbiology laboratories in Sweden. Species and subtype determination was achieved using small subunit ribosomal RNA and 60 kDa glycoprotein gene analysis. Samples were available for 398 patients, of whom 250 (63%) and 138 (35%) had acquired the infection in Sweden and abroad, respectively. Species identification was successful for 95% (379/398) of the samples, revealing 12 species/genotypes: Cryptosporidium parvum (n = 299), C. hominis (n = 49), C. meleagridis (n = 8), C. cuniculus (n = 5), Cryptosporidium chipmunk genotype I (n = 5), C. felis (n = 4), C. erinacei (n = 2), C. ubiquitum (n = 2), and one each of C. suis, C. viatorum, C. ditrichi, and Cryptosporidium horse genotype. One patient was co-infected with C. parvum and C. hominis. Subtyping was successful for all species/genotypes, except for C. ditrichi, and revealed large diversity, with 29 subtype families (including 4 novel ones: C. parvum IIr, IIs, IIt, and Cryptosporidium horse genotype VIc) and 81 different subtypes. The most common subtype families were IIa (n = 164) and IId (n = 118) for C. parvum and Ib (n = 26) and Ia (n = 12) for C. hominis. Infections caused by the zoonotic C. parvum subtype families IIa and IId dominated both in patients infected in Sweden and abroad, while most C. hominis cases were travel-related. Infections caused by non-hominis and non-parvum species were quite common (8%) and equally represented in cases infected in Sweden and abroad.

scholarly journals Base Pairing Constraints Drive Structural Epistasis in Ribosomal RNA Sequences

2010 ◽  
Vol 27 (8) ◽  
pp. 1868-1876 ◽  
Author(s):  
Julien Y. Dutheil ◽  
Fabrice Jossinet ◽  
Eric Westhof
Keyword(s):  
Ribosomal Rna ◽  
Base Pairing ◽  
Rna Sequences ◽  
Ribosomal Rna Sequences

PHYLOGENETIC RELATIONSHIPS IN GREEN PLANTS—A COMMENT ON THE USE OF 5S RIBOSOMAL RNA SEQUENCES BY BREMER ET AL.

Taxon ◽  
1988 ◽  
Vol 37 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Kelly P. Steele ◽  
Kent E. Holsinger ◽  
Robert K. Jansen ◽  
David W. Taylor
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Relationships ◽  
Rna Sequences ◽  
5S Ribosomal Rna ◽  
Green Plants ◽  
Ribosomal Rna Sequences

Phylogenetic Study of Haloanaerobic Bacteria by 16S Ribosomal RNA Sequences Analysis

1995 ◽  
Vol 18 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Tatjana P. Tourova ◽  
Eugenia S. Boulygina ◽  
Tatjana N. Zhilina ◽  
Richard S. Hanson ◽  
George A. Zavarzin
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Phylogenetic Study ◽  
Rna Sequences ◽  
Ribosomal Rna Sequences ◽  
Sequences Analysis
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