Effect of Cytokinins on Photosynthetic Pigments and Chlorophyllase Activity in in Vitro Cultures of Axillary Buds of Dianthus caryophyllus L.

1997 ◽  
Vol 16 (3) ◽  
pp. 169-172 ◽  
Author(s):  
T. Genkov ◽  
P. Tsoneva ◽  
I. Ivanova
Keyword(s):  
Photosynthetic Pigments ◽  
Dianthus Caryophyllus ◽  
In Vitro Cultures ◽  
Axillary Buds

scholarly journals Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

2016 ◽  
Vol 76 (1) ◽  
pp. 218-227 ◽  
Author(s):  
C. F. Resende ◽  
V. F. Braga ◽  
P. F. Pereira ◽  
C. J. Silva ◽  
V. F. Vale ◽  
...  
Keyword(s):  
Gas Exchange ◽  
Photosynthetic Pigments ◽  
Oxidative Metabolism ◽  
In Vitro Cultures ◽  
Metabolic Effects ◽  
In Vitro Cultivation ◽  
Antioxidant Enzymes Activities ◽  
Vitro Development ◽  
In Vitro Maintenance

Abstract This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA3 or 0.2 µM NAA, sucrose at 15 or 30 g L–1, in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA3 and 15 g L–1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L–1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.

Lignans from in vitro cultures of transgenic roots of Taxus x media var. Hicksii

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
K Sykłowska-Baranek ◽  
A Pietrosiuk ◽  
M Grech-Baran ◽  
M Bonfill ◽  
P Mistrzak
Keyword(s):  
In Vitro Cultures ◽  
Transgenic Roots ◽  
Taxus X Media

Phenolic compounds from in vitro cultures of Rindera graeca Boiss. & Feldr.

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
K Sykłowska-Baranek ◽  
A Pietrosiuk ◽  
K Graikou ◽  
H Damianakos ◽  
M Jeziorek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Cultures

HPTLC determination of catechins in in-vitro cultures of two species of the genusPhyllanthus

2008 ◽  
Vol 21 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Barbara Sparzak ◽  
Mirosława Krauze-Baranowska ◽  
Loretta Pobłocka-Olech
Keyword(s):  
In Vitro Cultures

Caffeoylquinic Acids with Potential Biological Activity from Plant In vitro Cultures as Alternative Sources of Valuable Natural Products

2020 ◽  
Vol 26 (24) ◽  
pp. 2817-2842
Author(s):  
Ewa Skała ◽  
Joanna Makowczyńska ◽  
Joanna Wieczfinska ◽  
Tomasz Kowalczyk ◽  
Przemysław Sitarek
Keyword(s):  
Secondary Metabolites ◽  
Cell Suspension ◽  
Plant Species ◽  
Plant Secondary Metabolites ◽  
Cell Suspension Cultures ◽  
In Vitro Cultures ◽  
Transformed Roots ◽  
Transgenic Roots ◽  
Plant In Vitro Cultures

Background: For a long time, the researchers have been looking for new efficient methods to enhance production and obtain valuable plant secondary metabolites, which would contribute to the protection of the natural environment through the preservation of various plant species, often rare and endangered. These possibilities offer plant in vitro cultures which can be performed under strictly-controlled conditions, regardless of the season or climate and environmental factors. Biotechnological methods are promising strategies for obtaining the valuable plant secondary metabolites with various classes of chemical compounds including caffeoylquinic acids (CQAs) and their derivatives. CQAs have been found in many plant species which are components in the daily diet and exhibit a wide spectrum of biological activities, including antioxidant, immunomodulatory, antihypertensive, analgesic, anti-inflammatory, hepato- and neuroprotective, anti-hyperglycemic, anticancer, antiviral and antimicrobial activities. They have also been found to offer protection against Alzheimer’s disease, and play a role in weight reduction and lipid metabolism control, as well as modulating the activity of glucose-6-phosphatase involved in glucose metabolism. Methods: This work presents the review of the recent advances in use in vitro cultures of various plant species for the alternative system to the production of CQAs and their derivatives. Production of the secondary metabolites in in vitro culture is usually performed with cell suspension or organ cultures, such as shoots and adventitious or transformed roots. To achieve high production of valuable secondary metabolites in in vitro cultures, the optimization of the culture condition is necessary with respect to both biomass accumulation and metabolite content. The optimization of the culture conditions can be achieved by choosing the type of medium, growth regulators or growth conditions, selection of high-productivity lines or culture period, supplementation of the culture medium with precursors or elicitor treatments. Cultivation for large-scale in bioreactors and genetic engineering: Agrobacterium rhizogenes transformation and expression improvement of transcriptional factor or genes involved in the secondary metabolite production pathway are also efficient strategies for enhancement of the valuable secondary metabolites. Results: Many studies have been reported to obtain highly productive plant in vitro cultures with respect to CQAs. Among these valuable secondary metabolites, the most abundant compound accumulated in in vitro cultures was 5-CQA (chlorogenic acid). Highly productive cultures with respect to this phenolic acid were Leonurus sibiricus AtPAP1 transgenic roots, Lonicera macranthoides and Eucomia ulmoides cell suspension cultures which accumulated above 20 mg g-1 DW 5-CQA. It is known that di- and triCQAs are less common in plants than monoCQAs, but it was also possible to obtain them by biotechnological methods. Conclusion: The results indicate that the various in vitro cultures of different plant species can be a profitable approach for the production of CQAs. In particular, an efficient production of these valuable compounds is possible by Lonicera macranthoides and Eucomia ulmoides cell suspension cultures, Leonurus sibiricus transformed roots and AtPAP1 transgenic roots, Echinacea angustifolia adventitious shoots, Rhaponticum carthamoides transformed plants, Lavandula viridis shoots, Sausera involucrata cell suspension and Cichorium intybus transformed roots.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

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