A Group I Intron in the Nuclear Small Subunit rRNA Gene of Cryptendoxyla hypophloia, an Ascomycetous Fungus: Evidence for a New Major Class of Group I Introns

1999 ◽  
Vol 48 (5) ◽  
pp. 493-500 ◽  
Author(s):  
Sung-Oui Suh ◽  
Kevin G. Jones ◽  
Meredith Blackwell
Keyword(s):  
Group I Intron ◽  
Small Subunit ◽  
Rrna Gene ◽  
Group I Introns ◽  
Small Subunit Rrna ◽  
Small Subunit Rrna Gene ◽  
Major Class ◽  
Group I ◽  
Ascomycetous Fungus

Reverse splicing of a mobile twin-ribozyme group I intron into the natural small subunit rRNA insertion site

2005 ◽  
Vol 33 (3) ◽  
pp. 482-484 ◽  
Author(s):  
Å.B. Birgisdottir ◽  
S.D. Johansen
Keyword(s):  
Horizontal Transfer ◽  
Homing Endonuclease ◽  
Insertion Site ◽  
Group I Intron ◽  
Small Subunit ◽  
Group I Introns ◽  
Small Subunit Rrna ◽  
Homing Endonuclease Gene ◽  
Group I ◽  
Endonuclease Gene

A mobile group I intron containing two ribozyme domains and a homing endonuclease gene (twin-ribozyme intron organization) can integrate by reverse splicing into the small subunit rRNA of bacteria and yeast. The integration is sequence-specific and corresponds to the natural insertion site (homing site) of the intron. The reverse splicing is independent of the homing endonuclease gene, but is dependent on the group I splicing ribozyme domain. The observed distribution of group I introns in nature can be explained by horizontal transfer between natural homing sites by reverse splicing and subsequent spread in populations by endonuclease-dependent homing.

Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences

2003 ◽  
Vol 126 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Christophe Noël ◽  
Corinne Peyronnet ◽  
Delphine Gerbod ◽  
Virginia P Edgcomb ◽  
Pilar Delgado-Viscogliosi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

scholarly journals A Unique Group I Intron in Coxiella burnetii Is a Natural Splice Mutant

2009 ◽  
Vol 191 (12) ◽  
pp. 4044-4046 ◽  
Author(s):  
Rahul Raghavan ◽  
Linda D. Hicks ◽  
Michael F. Minnick
Keyword(s):  
Coxiella Burnetii ◽  
Group I Intron ◽  
23S Rrna ◽  
Rrna Gene ◽  
Group I Introns ◽  
Group I ◽  
23S Rrna Gene ◽  
Self Splicing

ABSTRACT Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3′-terminal adenine in place of a conserved guanine. Here, we show that, unlike all other group I introns, Cbu.L1917 utilizes a different cofactor for each splicing step and has a decreased self-splicing rate in vitro.

Genetic interrelationships of proteolyticClostridium botulinum types A, B, and F and other members of theClostridium botulinum complex as revealed by small-subunit rRNA gene sequences

1994 ◽  
Vol 64 (3-4) ◽  
pp. 273-283 ◽  
Author(s):  
R. A. Hutson ◽  
D. E. Thompson ◽  
P. A. Lawson ◽  
R. P. Schocken-Itturino ◽  
E. C. B�ttger ◽  
...  
Keyword(s):  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

Free-living nematodes associated with pine cones of Pinus thunbergii and P. taeda at Japanese coastal and inland forest sites

Nematology ◽  
2019 ◽  
Vol 21 (4) ◽  
pp. 389-400 ◽  
Author(s):  
Yudai Kitagami ◽  
Natsumi Kanzaki ◽  
Toko Tanikawa ◽  
Yosuke Matsuda
Keyword(s):  
Gene Sequence ◽  
Small Subunit ◽  
Pinus Thunbergii ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Free Living ◽  
Small Subunit Rrna Gene ◽  
Forest Sites ◽  
High Bootstrap ◽  
Pine Cones

Summary We surveyed the distribution of nematodes in 56 cones of Pinus thunbergii collected from both live branches and on the forest floor in three coastal and inland habitats and in 11 cones of P. taeda collected at different heights. We identified 47 nematodes to family or genera by analysis of an 18S small subunit rRNA gene sequence. The frequencies of occurrence of free-living cone nematodes were 97% in coastal P. thunbergii, 92% in inland P. thunbergii, and 82% in P. taeda. Phylogenetic analysis assigned the nematodes to four clades with high bootstrap values. Nine sequences that were found only in cones on live branches were clustered with Panagrobelus stammeri and an unknown Panagrobelus sp. Our results imply that nematodes are commonly associated with cones in pine forest ecosystems and that a capacity for anhydrobiosis may be a key to surviving above-ground.

The molecular evidence of Babesia microti infection in small mammals collected in Slovenia

Parasitology ◽  
2003 ◽  
Vol 126 (2) ◽  
pp. 113-117 ◽  
Author(s):  
D. DUH ◽  
M. PETROVEC ◽  
T. TRILAR ◽  
T. AVSIC-ZUPANC
Keyword(s):  
Small Mammals ◽  
Small Subunit ◽  
Clethrionomys Glareolus ◽  
Babesia Microti ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Pcr Assay ◽  
Small Subunit Rrna Gene ◽  
Yellow Necked Mouse

In Europe, the zoonotic cycle of Babesia microti has not been determined so far. Recently, B. microti was detected in Ixodes ricinus ticks in Slovenia by using molecular methods. In order to investigate the mammalian hosts of B. microti in Slovenia we collected 261 small mammals representing 11 species. They were tested for the presence of babesial parasites with a PCR assay based on the nuclear small subunit rRNA gene (nss-rDNA). The bank vole (Clethrionomys glareolus) and yellow-necked mouse (Apodemus flavicollis) were infected with B. microti. The prevalence rate was 15·9% for C. glareolus and 11·8% for A. flavicollis. Nucleotide sequences of amplified portions of B. microti nss-rDNA from C. glareolus and A. flavicollis were indistinguishable from each other and identical with those previously described in I. ricinus ticks collected in Slovenia. The results of this study represent molecular evidence of B. microti in small mammals in Europe.

Sign in / Sign up

Export Citation Format

Share Document