Intron Distribution in Ancient Paralogs Supports Random Insertion and Not Random Loss

1997 ◽  
Vol 44 (6) ◽  
pp. 573-584 ◽  
Author(s):  
Glen Cho ◽  
Russell F. Doolittle
Keyword(s):  
Random Loss ◽  
Random Insertion ◽  
Intron Distribution

TANDEM DUPLICATION RANDOM LOSS EVENTS INFLUENCED TO GENE ORDER CHANGES IN BAIKALIAN AMPHIPODS

2018 ◽  
Author(s):  
E.A. SIROTININA ◽  
◽  
E.V. ROMANOVA ◽  
D. YU. SHERBAKOV ◽  
◽  
...  
Keyword(s):  
Gene Order ◽  
Tandem Duplication ◽  
Random Loss

A Novel Ty1-Mediated Fragmentation Method for Native and Artificial Yeast Chromosomes Reveals That the Mouse Steel Gene is a Hotspot for Ty1 Integration

Genetics ◽  
1996 ◽  
Vol 143 (2) ◽  
pp. 673-683
Author(s):  
Jacob Z Dalgaard ◽  
Mukti Banerjee ◽  
M Joan Curcio
Keyword(s):  
Transcription Unit ◽  
Yeast Genome ◽  
Physical Analysis ◽  
Single Chromosome ◽  
Artificial Chromosomes ◽  
Unique Site ◽  
High Fraction ◽  
A Site ◽  
Yeast Artificial Chromosomes ◽  
Random Insertion

Abstract We have developed a powerful new tool for the physical analysis of genomes called Ty1-mediated chromosomal fragmentation and have used the method to map 24 retrotransposon insertions into two different mousederived yeast artificial chromosomes (YACs). Expression of a plasmid-encoded GAL1:Ty1 fusion element marked with the retrotransposition indicator gene, ade2AI, resulted in a high fraction of cells that sustained a single Ty1 insertion marked with ADE2. Strains in which Ty1ADE2 inserted into aYAC were identified by cosegregation of the ADE2 gene with the URA3-marked YAC. Ty1ADE2 elements also carried a site for the endonuclease I-DmoI, which we demonstrate is not present anywhere in the yeast genome. Consequently, I-DmoI cleaved a single chromosome or YAC at the unique site of Ty1ADE2 insertion, allowing rapid mapping of integration events. Our analyses showed that the frequency of Ty1ADE2 integration into YACs is equivalent to or higher than that expected based on random insertion. Remarkably, the 50-kb transcription unit of the mouse Steel locus was shown to be a highly significant hotspot for Ty1 integration. The accessibility of mammalian transcription units to Ty1 insertion stands in contrast to that of yeast transcription units.

scholarly journals A Novel Gene Involved in the Survival of Streptococcus mutans under Stress Conditions

2013 ◽  
Vol 80 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Dan Li ◽  
Yukie Shibata ◽  
Toru Takeshita ◽  
Yoshihisa Yamashita
Keyword(s):  
Streptococcus Mutans ◽  
Streptococcus Pyogenes ◽  
Biofilm Formation ◽  
Acid Tolerance ◽  
Stress Conditions ◽  
Insertion Mutagenesis ◽  
Trna Modification ◽  
Content Type ◽  
Virulence Proteins ◽  
Random Insertion

ABSTRACTAStreptococcus mutansmutant defective in aciduricity was constructed by random-insertion mutagenesis. Sequence analysis of the mutant revealed a mutation ingidA, which is known to be involved in tRNA modification inStreptococcus pyogenes. Complementation ofgidAbyS. pyogenesgidArecovered the acid tolerance ofS. mutans. Although thegidA-inactivatedS. pyogenesmutant exhibited significantly reduced expression of multiple extracellular virulence proteins, theS. mutansmutant did not. On the other hand, thegidAmutant ofS. mutansshowed reduced ability to withstand exposure to other stress conditions (high osmotic pressure, high temperature, and bacitracin stress) besides an acidic environment. In addition, loss of GidA decreased the capacity for glucose-dependent biofilm formation by over 50%. This study revealed thatgidAplays critical roles in the survival ofS. mutansunder stress conditions, including lower pH.

scholarly journals Random Mutagenesis of Clostridium cellulolyticum by Using a Tn1545 Derivative

2010 ◽  
Vol 76 (13) ◽  
pp. 4546-4549 ◽  
Author(s):  
Jean-Charles Blouzard ◽  
Odile Valette ◽  
Chantal Tardif ◽  
Pascale de Philip
Keyword(s):  
Cell Wall ◽  
Metabolic Engineering ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Random Mutagenesis ◽  
Cell Wall Degradation ◽  
Clostridium Cellulolyticum ◽  
Plant Cell Wall Degradation ◽  
Random Insertion

ABSTRACT Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.

Random Insertion and Deletion Mutagenesis

2003 ◽  
pp. 53-64 ◽  
Author(s):  
Hiroshi Murakami ◽  
Takahiro Hohsaka ◽  
Masahiko Sisido
Keyword(s):  
Deletion Mutagenesis ◽  
Insertion And Deletion ◽  
Random Insertion

scholarly journals Agrobacterium tumefaciens-Mediated Transformation ofValsa mali: An Efficient Tool for Random Insertion Mutagenesis

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Caixia Wang ◽  
Xiangnan Guan ◽  
Hanyan Wang ◽  
Guifang Li ◽  
Xiangli Dong ◽  
...  
Keyword(s):  
Single Gene ◽  
Economic Losses ◽  
Insertion Mutagenesis ◽  
Transformation System ◽  
Canker Disease ◽  
Germination Ability ◽  
First Time ◽  
Insight Into ◽  
Random Insertion ◽  
Valsa Mali

Valsa maliis a causal agent of apple and pear trees canker disease, which is a destructive disease that causes serious economic losses in eastern Asia, especially in China. The lack of an efficient transformation system forValsa maliretards its investigation, which poses difficulties to control the disease. In this research, a transformation system for this pathogen was established for the first time usingA. tumefaciens-mediated transformation (ATMT), with the optimal transformation conditions as follows: 106/mL conidia suspension, cocultivation temperature 22°C, cocultivation time 72 hours, and 200 μM acetosyringone (AS) in the inductive medium. The average transformation efficiency was 1015.00 ± 37.35 transformants per 106recipient conidia. Thirty transformants were randomly selected for further confirmation and the results showed the presence of T-DNA in all hygromycin B resistant transformants and also revealed random and single gene integration with genetic stability. Compared with wild-type strain, those transformants exhibited various differences in morphology, conidia production, and conidia germination ability. In addition, pathogenicity assays revealed that 14 transformants had mitigated pathogenicity, while one had enhanced infection ability. The results suggest that ATMT ofV. maliis a useful tool to gain novel insight into this economically important pathogen at molecular levels.

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