A quantitative analysis of the glial cell reaction in primary sensory termination areas following sciatic nerve injury and treatment with nerve growth factor in the adult rat

1997 ◽  
Vol 114 (3) ◽  
pp. 393-404 ◽  
Author(s):  
N. P. Eriksson ◽  
Jonas Karl Erik Persson ◽  
Håkan Aldskogius ◽  
Mikael Svensson
Keyword(s):  
Quantitative Analysis ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sciatic Nerve ◽  
Glial Cell ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Cell Reaction ◽  
Nerve Growth ◽  
Adult Rat

Repairing rat sciatic nerve injury by a nerve-growth-factor-loaded, chitosan-based nerve conduit

2012 ◽  
Vol 59 (5) ◽  
pp. 388-394 ◽  
Author(s):  
Hongkui Wang ◽  
Qing Zhao ◽  
Weijia Zhao ◽  
Qiong Liu ◽  
Xiaosong Gu ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Nerve Conduit ◽  
Nerve Growth ◽  
Rat Sciatic Nerve

Neural mobilization promotes nerve regeneration by nerve growth factor and myelin protein zero increased after sciatic nerve injury

2014 ◽  
Vol 33 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Joyce Teixeira da Silva ◽  
Fabio Martinez dos Santos ◽  
Aline Caroline Giardini ◽  
Daniel de Oliveira Martins ◽  
Mara Evany de Oliveira ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Myelin Protein ◽  
Myelin Protein Zero ◽  
Nerve Growth ◽  
Protein Zero

Nerve Growth Factor of Red Nucleus Involvement in Pain Induced by Spared Nerve Injury of the Rat Sciatic Nerve

2009 ◽  
Vol 34 (9) ◽  
pp. 1612-1618 ◽  
Author(s):  
Yuan-Yuan Jing ◽  
Jun-Yang Wang ◽  
Xiao-Li Li ◽  
Zhi-Hong Wang ◽  
Liu Pei ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Red Nucleus ◽  
Spared Nerve Injury ◽  
Nerve Growth ◽  
Rat Sciatic Nerve

scholarly journals 3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Diego Noé Rodríguez-Sánchez ◽  
Giovana Boff Araujo Pinto ◽  
Luciana Politti Cartarozzi ◽  
Alexandre Leite Rodrigues de Oliveira ◽  
Ana Livia Carvalho Bovolato ◽  
...  
Keyword(s):  
Growth Factor ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Sciatic Nerve Injury ◽  
Motor Deficits ◽  
3D Printed

Abstract Background Nerve injuries are debilitating, leading to long-term motor deficits. Remyelination and axonal growth are supported and enhanced by growth factor and cytokines. Combination of nerve guidance conduits (NGCs) with adipose-tissue-derived multipotent mesenchymal stromal cells (AdMSCs) has been performing promising strategy for nerve regeneration. Methods 3D-printed polycaprolactone (PCL)-NGCs were fabricated. Wistar rats subjected to critical sciatic nerve damage (12-mm gap) were divided into sham, autograft, PCL (empty NGC), and PCL + MSCs (NGC multi-functionalized with 106 canine AdMSCs embedded in heterologous fibrin biopolymer) groups. In vitro, the cells were characterized and directly stimulated with interferon-gamma to evaluate their neuroregeneration potential. In vivo, the sciatic and tibial functional indices were evaluated for 12 weeks. Gait analysis and nerve conduction velocity were analyzed after 8 and 12 weeks. Morphometric analysis was performed after 8 and 12 weeks following lesion development. Real-time PCR was performed to evaluate the neurotrophic factors BDNF, GDNF, and HGF, and the cytokine and IL-10. Immunohistochemical analysis for the p75NTR neurotrophic receptor, S100, and neurofilament was performed with the sciatic nerve. Results The inflammatory environment in vitro have increased the expression of neurotrophins BDNF, GDNF, HGF, and IL-10 in canine AdMSCs. Nerve guidance conduits multi-functionalized with canine AdMSCs embedded in HFB improved functional motor and electrophysiological recovery compared with PCL group after 12 weeks. However, the results were not significantly different than those obtained using autografts. These findings were associated with a shift in the regeneration process towards the formation of myelinated fibers. Increased immunostaining of BDNF, GDNF, and growth factor receptor p75NTR was associated with the upregulation of BDNF, GDNF, and HGF in the spinal cord of the PCL + MSCs group. A trend demonstrating higher reactivity of Schwann cells and axonal branching in the sciatic nerve was observed, and canine AdMSCs were engrafted at 30 days following repair. Conclusions 3D-printed NGCs multi-functionalized with canine AdMSCs embedded in heterologous fibrin biopolymer as cell scaffold exerted neuroregenerative effects. Our multimodal approach supports the trophic microenvironment, resulting in a pro-regenerative state after critical sciatic nerve injury in rats.

Nerve growth factor (NGF) content in adult rat brain tissues is several-fold higher than generally reported and is largely associated with sedimentable fractions

1996 ◽  
Vol 728 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Marius C. Hoener ◽  
Eleanore Hewitt ◽  
James M. Conner ◽  
James W. Costello ◽  
Silvio Varon
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Brain ◽  
Nerve Growth ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Brain Tissues
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