Biological activation of NG-nitro-D-arginine by kidney homogenate

1997 ◽  
Vol 356 (4) ◽  
pp. 495-499 ◽  
Author(s):  
Xing Cheng ◽  
Yong-Xiang Wang ◽  
C. C. Y. Pang
Keyword(s):  
Kidney Homogenate

scholarly journals EXPERIMENTAL ALLERGIC GLOMERULONEPHRITIS INDUCED IN THE RABBIT WITH HOMOLOGOUS RENAL ANTIGENS

1967 ◽  
Vol 125 (1) ◽  
pp. 163-176 ◽  
Author(s):  
Emil R. Unanue ◽  
Frank J. Dixon ◽  
Joseph D. Feldman
Keyword(s):  
Renal Injury ◽  
Passive Transfer ◽  
Basement Membranes ◽  
Kidney Homogenate ◽  
Autoantibody Response ◽  
Renal Tubular ◽  
Renal Antigens ◽  
Experimental Allergic

Rabbits immunized to several homologous renal antigens developed a variety of autoantikidney antibodies. Some of these antibodies reacted with the host's glomeruli and appeared to cause glomerulonephritis. Passive transfer of sera from some of these nephritic rabbits into normal, unilaterally nephrectomized rabbits led to the induction of nephritis. The production of autoantibody to glomeruli was transitory in most instances in spite of continued immunization. In some rabbits immunized to whole kidney homogenate, extracts or sediment, antibodies were found fixed to renal tubular basement membranes where an ultrastructural lesion was demonstrated. Rabbits also produced antikidney antibody apparently to tubular cytoplasmic components which did not fix to kidney in vivo and were of no pathogenetic significance. Immunization with autologous renal basement membranes induced a small autoantibody response in half the rabbits. This response was not associated with detectable renal injury.

METHYLATION OF THE 3-OH POSITION OF CATECHOL ACIDS BY RAT LIVER AND KIDNEY PREPARATIONS

1958 ◽  
Vol 36 (5) ◽  
pp. 491-497 ◽  
Author(s):  
J. Pellerin ◽  
A. D'Iorio
Keyword(s):  
Enzymatic Activity ◽  
Rat Liver ◽  
Paper Chromatography ◽  
Reduced Glutathione ◽  
Liver Homogenate ◽  
Supernatant Fraction ◽  
Hydroxybenzoic Acid ◽  
Dihydroxybenzoic Acid ◽  
Kidney Homogenate ◽  
Liver And Kidney

3,4-Dihydroxybenzoic acid, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxymandelic acid, and 3,4-dihydroxycinnamic acid were separately incubated with L-methionine-methyl-C14 in the presence of rat liver or kidney homogenate. In each case, the radioactive metabolite separated by paper chromatography was found to have migrating properties similar to those of the 3-methoxy-4-hydroxyphenolic acid. This reaction was enhanced by the addition of ATP, Mg++, and reduced glutathione. When 3-hydroxybenzoic acid was incubated in this medium no methylated derivative was obtained. Preliminary experiments indicated that the enzymatic activity was contained mostly in the supernatant fraction. It was also noted that liver homogenate was much more active than kidney homogenate in methylating catechol acids.

Glutamine and Glutaminase in Blood

1956 ◽  
Vol 2 (6) ◽  
pp. 413-419 ◽  
Author(s):  
Frank L Iber ◽  
Joseph Bruton
Keyword(s):  
Kidney Homogenate ◽  
Accurate Procedure ◽  
Glutaminase Activity

Abstract A convenient and accurate procedure for the determination of glutaminase activity and glutamine in blood has been described. Kidney homogenate from rats made acidotic by feeding NH4CL was used as a glutaminase source and the Conway microdiffusion method was used to determine ammonia.

On the metabolism of ϵ-N-methyl-l-lysine by rat-kidney homogenate

1963 ◽  
Vol 71 ◽  
pp. 745-747 ◽  
Author(s):  
Sangduk Kim ◽  
Leo Benoiton ◽  
Woon ki Paik
Keyword(s):  
Rat Kidney ◽  
Kidney Homogenate

Conversion of L-Thyroxine to Triiodothyronine in Rat Kidney Homogenate*

Endocrinology ◽  
1978 ◽  
Vol 102 (2) ◽  
pp. 612-622 ◽  
Author(s):  
P. CHIRASEVEENUPRAPUND ◽  
U. BUERGI ◽  
A. GOSWAMI ◽  
I. N. ROSENBERG
Keyword(s):  
Rat Kidney ◽  
Kidney Homogenate

scholarly journals NEPHROTOXIC SERUM NEPHRITIS IN RATS

1956 ◽  
Vol 104 (4) ◽  
pp. 487-499 ◽  
Author(s):  
Howard C. Goodman ◽  
James H. Baxter
Keyword(s):  
Skeletal Muscle ◽  
Ammonium Sulfate ◽  
Protective Factor ◽  
Rat Kidney ◽  
Tryptic Digestion ◽  
Tissue Antigen ◽  
Rat Lung ◽  
High Concentration ◽  
Kidney Homogenate ◽  
Rat Tissue

A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60°, destroyed by boiling, is non-dialyzable, can be precipitated by ammonium sulfate, but resists destruction by proteolytic and other enzymes. In accord with previous studies on the organ localization of the rat tissue antigen responsible for the production of nephrotoxic antibodies, the soluble protective factor, or antigen derivative, can be obtained by tryptic digestion of rat lung, skeletal muscle, heart, and liver.

scholarly journals Anti-urolithiatic Activity of Sambong (Blumea balsamifera) Extract in Ethylene Glycol-induced Urolithiatic Wistar Rats (Rattus norvegicus)

2020 ◽  
Vol 54 (1) ◽  
Author(s):  
Althea Samantha C. Agdamag ◽  
Larielyn Hope C. Aggabao ◽  
Mary Sheena C. Agudo ◽  
Francis Louis M. Alcachupas ◽  
Jeremiah Carlo V. Alejo ◽  
...  
Keyword(s):  
Treatment Group ◽  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Stone Formation ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Calcium Oxalate Stone ◽  
Kidney Homogenate ◽  
Calcium Oxalate Stones

Objective. The study aimed to determine if Blumea balsamifera inhibits calcium oxalate stone formation in the kidneys through determination of the number of calcium oxalate stones in the renal cortex and the percent mass of calcium oxalate. Methods. Post-test only control group design was used using five treatment groups with placebo as the negative control, potassium citrate as the positive control, and 50%, 100%, and 200% sambong treatment. Urolithiasis was induced through ethylene glycol and ammonium chloride. Each treatment group was administered its corresponding treatment solution once daily for twenty-one days. Histopathologic examination and kidney homogenate analysis were done to determine the degree of deposition of calcium oxalate stones in renal tissues and the oxalate content, respectively. Statistical analyses were performed using one-way ANOVA and post hoc Gabriel's Pairwise Comparisons Test. Results. The 100% sambong treatment group showed the least mean number of stones while the positive control and 50% sambong treatment group exhibited the highest anti-urolithiatic activity in terms of oxalate content of the kidney homogenate. Conclusion. It can be concluded from the study that Blumea balsamifera inhibits calcium oxalate stone formation in the kidneys with the 100% and 50% sambong treatment most effective in decreasing number of stones and oxalate content of the kidney homogenate, respectively.

scholarly journals PORPHYRIN BIOSYNTHESIS FROM DELTA-AMINOLEVULINIC ACID BY THE KIDNEY HOMOGENATE OF LEAD POISONED RABBITS

1969 ◽  
Vol 7 (1-2) ◽  
pp. 46-53
Author(s):  
Shosuke SUZUKI ◽  
Goro YOKOHASHI ◽  
Tsuguyoshi SUZUKI
Keyword(s):  
Aminolevulinic Acid ◽  
Kidney Homogenate
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