Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements

2001 ◽  
Vol 58 (3) ◽  
pp. 403-419 ◽  
Author(s):  
B. Berger ◽  
D. Haas
Keyword(s):  
Insertion Sequence ◽  
Bacterial Insertion Sequence

The early phase of a bacterial insertion sequence infection

2010 ◽  
Vol 78 (4) ◽  
pp. 278-288 ◽  
Author(s):  
Manuel Bichsel ◽  
Andrew D. Barbour ◽  
Andreas Wagner
Keyword(s):  
Early Phase ◽  
Insertion Sequence ◽  
Bacterial Insertion Sequence

Translational control of transposition activity of the bacterial insertion sequence IS1.

1991 ◽  
Vol 10 (3) ◽  
pp. 705-712 ◽  
Author(s):  
J. M. Escoubas ◽  
M. F. Prère ◽  
O. Fayet ◽  
I. Salvignol ◽  
D. Galas ◽  
...  
Keyword(s):  
Translational Control ◽  
Insertion Sequence ◽  
Transposition Activity ◽  
Bacterial Insertion Sequence ◽  
Control Of Transposition

Transposase-induced excision and circularization of the bacterial insertion sequence IS911.

1992 ◽  
Vol 11 (13) ◽  
pp. 5079-5090 ◽  
Author(s):  
P. Polard ◽  
M.F. Prère ◽  
O. Fayet ◽  
M. Chandler
Keyword(s):  
Insertion Sequence ◽  
Bacterial Insertion Sequence

LOCALIZATION OF ACTION OF THE IS50-ENCODED TRANSPOSASE PROTEIN

Genetics ◽  
1986 ◽  
Vol 112 (3) ◽  
pp. 421-427
Author(s):  
Suhas H Phadnis ◽  
Chihiro Sasakawa ◽  
Douglas E Berg
Keyword(s):  
Dna Sequence ◽  
Insertion Sequence ◽  
The Other ◽  
Second Step ◽  
Direct Repeats ◽  
Dna Molecules ◽  
Protein Tracking ◽  
Bacterial Insertion Sequence ◽  
End Use ◽  
In Cis

ABSTRACT The movement of the bacterial insertion sequence IS50 and of composite elements containing direct terminal repeats of IS50 involves the two ends of IS50, designated O (outside) and I (inside), which are weakly matched in DNA sequence, and an IS50 encoded protein, transposase, which recognizes the O and I ends and acts preferentially in cis. Previous data had suggested that, initially, transposase interacts preferentially with the O end sequence and then, in a second step, with either an O or an I end. To better understand the cis action of transposase and how IS50 ends are selected, we generated a series of composite transposons which contain direct repeats of IS50 elements. In each transposon, one IS50 element encoded transposase (tnp  +), and the other contained a null (tnp  -) allele. In each of the five sets of composite transposons studied, the transposon for which the tnp  + IS50 element contained its O end was more active than a complementary transposon for which the tnp  - IS50 element contained its O end. This pattern of O end use suggests models in which the cis action of transposase and its choice of ends is determined by protein tracking along DNA molecules.

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