Cellular uptake of long-chain fatty acids: role of membrane-associated fatty-acid-binding/transport proteins

2000 ◽  
Vol 57 (10) ◽  
pp. 1360-1372 ◽  
Author(s):  
A. K. Dutta-Roy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cellular Uptake ◽  
Transport Proteins ◽  
Long Chain Fatty Acids ◽  
Fatty Acid Binding ◽  
Long Chain ◽  
Chain Fatty Acids

Z protein in hepatic uptake and esterification of long-chain fatty acids

1975 ◽  
Vol 228 (6) ◽  
pp. 1634-1640 ◽  
Author(s):  
S Mishkin ◽  
L Stein ◽  
G Fleischner ◽  
Z Gatmaitan ◽  
IM Arias
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Hepatic Uptake ◽  
Perfused Liver ◽  
Long Chain Fatty Acids ◽  
Fatty Acid Binding ◽  
Liver Preparation ◽  
Z Protein ◽  
Long Chain ◽  
Chain Fatty Acids

Fatty acids radioactivity was bound to Z protein in liver after administration of['3H]oleate to rats or to a perfused rat liver preparation. Pretreatment withflavaspidic acid (340 mumol/kg), a potent inhibitor of fatty acid binding to hepatic Zprotein in vitri, effectively reduced oleate radioactivity bound to Z by 90.2 plusor minus 4.3% and 85.0 plus or minus 6.2% in the intact rat and perfused liver, respectively. In spite of this effect, pretreatment of rats with flavaspidic acid did notalter plasma clearance, hepatic uptake, and esterification of ['3H]oleate. In contrast, in the perfused liver preparation, infusion of flavaspidic acid (340 mumol/kg)or bromosulphalein (360 mumol/kg) increased uptake of ['3H]oleate at least twofold,and oleate esterification was decreased by 15-30%. These results suggest that the binding of long-chain fatty acids to Z protein is not an obligatory step in their uptakeby the liver and that Z protein may be involved in fatty acid esterification.

scholarly journals A continuous fluorescence displacement assay for the measurement of phospholipase A2 and other lipases that release long-chain fatty acids

1990 ◽  
Vol 266 (2) ◽  
pp. 435-439 ◽  
Author(s):  
D C Wilton
Keyword(s):  
Fatty Acids ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Phospholipase A2 ◽  
Long Chain Fatty Acids ◽  
Fatty Acid Binding ◽  
Displacement Assay ◽  
Hydrolysis Of ◽  
Long Chain ◽  
Chain Fatty Acids

1. A new continuous fluorescence assay for phospholipase A2 is described which involves the displacement of the highly fluorescent fatty-acid probe 11-(dansylamino)undecanoic acid from rat liver fatty-acid-binding protein by long-chain fatty acids released as a result of phospholipase A2-catalysed hydrolysis of phospholipids. The initial rate of decrease in fluorescence is linearly related to enzyme activity. 2. The assay will detect enzyme activity down to about 10 pmol/min per ml and gives a linear response up to about 10 nmol/min per ml. 3. The assay will work with all phospholipids that have been tested including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and phosphatidylglycerol. Substrates carrying a net negative charge showed the highest rates of hydrolysis. 4. The assay will work, in principle, with an enzyme catalysing the release of long-chain fatty acids from a fatty-acylated substrate. This has been confirmed with pancreatic lipase and cholesterol esterase.

Role of Fatty Acid Binding Proteins and Long Chain Fatty Acids in Modulating Nuclear Receptors and Gene Transcription

Lipids ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 1-17 ◽  
Author(s):  
Friedhelm Schroeder ◽  
Anca D. Petrescu ◽  
Huan Huang ◽  
Barbara P. Atshaves ◽  
Avery L. McIntosh ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Nuclear Receptors ◽  
Gene Transcription ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Long Chain Fatty Acids ◽  
Fatty Acid Binding ◽  
Long Chain

Fatty acid regulation of fatty acid-binding protein expression in the small intestine

1997 ◽  
Vol 273 (2) ◽  
pp. G289-G295 ◽  
Author(s):  
H. Poirier ◽  
I. Niot ◽  
P. Degrace ◽  
M. C. Monnot ◽  
A. Bernard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Small Intestine ◽  
Fatty Acid ◽  
Sunflower Oil ◽  
Long Chain Fatty Acids ◽  
Fatty Acid Binding ◽  
Fabp Gene ◽  
Long Chain ◽  
Chain Fatty Acids

The effects of dietary oil intake and fatty acid infusions on the expression of intestinal and liver fatty acid-binding proteins (I-FABP and L-FABP, respectively) were investigated in the small intestine of mice. A daily force-feeding for 7 days with 0.2 ml sunflower oil specifically increased L-FABP mRNA and protein levels in duodenum and proximal jejunum. This upregulation was mediated in time- and dose-dependent manners by a minute quantity of linoleic acid, the main fatty acid found in sunflower oil. The L-FABP induction was only found with long-chain fatty acids, with the nonmetabolizable, substituted fatty acid alpha-bromopalmitate being far more active. A hormonally mediated effect is unlikely because long-chain fatty acids induced L-FABP mRNA in the Caco-2 cell line cultured in serum-free medium. Therefore, long-chain fatty acids are strong inducers of L-FABP gene expression in the small intestine. In contrast to data found in the rat, I-FABP gene expression appears to be unaffected by a lipid-enriched diet in the mouse.

Titration and Exchange Studies of Liver Fatty Acid-Binding Protein with13C-Labeled Long-Chain Fatty Acids†

Biochemistry ◽  
2002 ◽  
Vol 41 (17) ◽  
pp. 5453-5461 ◽  
Author(s):  
Hsin Wang ◽  
Yan He ◽  
Christopher D. Kroenke ◽  
Sarala Kodukula ◽  
Judith Storch ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Long Chain Fatty Acids ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Long Chain ◽  
Chain Fatty Acids
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