Limulus hemocyanin, an unusual ligand for myelin basic protein from central nervous system

1997 ◽  
Vol 53 (4) ◽  
pp. 291-293 ◽  
Author(s):  
U. Gröschel-Stewart ◽  
R. Glenz ◽  
D. Haney
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Myelin Basic Protein ◽  
Basic Protein

A developmentally regulated DNA-binding protein from mouse brain stimulates myelin basic protein gene expression

1993 ◽  
Vol 13 (5) ◽  
pp. 3103-3112
Author(s):  
S Haas ◽  
J Gordon ◽  
K Khalili
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Dna Binding ◽  
Myelin Basic Protein ◽  
Mouse Brain ◽  
Basic Protein ◽  
Binding Protein ◽  
Cell Type ◽  
A Cell ◽  
Cell Type Specific

Transcription of the myelin basic protein (MBP) gene is regulated in a cell-type-specific and developmental stage-specific manner during myelin formation in the murine central nervous system. The 5'-flanking region of the MBP gene contains several regulatory elements that differentially contribute to the cell-type-specific transcription of MBP in cells derived from the central nervous system. The proximal element, termed MB1, which is located between nucleotides -14 and -50 with respect to the RNA start site, has previously been shown to have characteristics of a cell-type-specific enhancer element. In this study, we used band shift and UV cross-linking assays to identify DNA-binding proteins in mouse brain nuclear extract which interact with the MB1 element. Fractionation of these extracts has allowed the identification of a 38- to 41-kDa nuclear protein, derived from mouse brain tissue at the peak of myelination, which specifically binds the MB1 DNA sequence. Fractions enriched in the MB1-binding protein have been shown to stimulate transcription of the MBP promoter in extract derived from HeLa cells. MB1 binding protein activity is expressed in a tissue-specific and development stage-specific pattern which coincides with the pattern of MBP transcription, suggesting that this protein may be a biologically relevant transcription factor for the MBP gene in vivo.

scholarly journals Immunocytochemical localization studies of myelin basic protein.

1978 ◽  
Vol 76 (2) ◽  
pp. 502-511 ◽  
Author(s):  
J R Mendell ◽  
J N Whitaker
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Horseradish Peroxidase ◽  
Myelin Basic Protein ◽  
Light Microscope ◽  
Basic Protein ◽  
Tissue Fixation ◽  
Immunocytochemical Localization ◽  
Structural Studies ◽  
Immunocytochemical Studies

The location of myelin encephalitogenic or basic protein (BP) in peripheral nervous system (PNS) and central nervous system (CNS) was investigated by immunofluorescence and horseradish peroxidase (HRP) immunocytochemistry. BP or cross-reacting material could be clearly localized to myelin by immunofluorescence and light microscope HRP immunocytochemistry. Fine structural studies proved to be much more difficult, especially in the CNS, due to problems in tissue fixation and penetration of reagents. Sequential fixation in aldehyde followed by ethanol or methanol provided the best conditions for ultrastructural indirect immunocytochemical studies. In PNS tissue, anti-BP was localized exclusively to the intraperiod line of myelin. Because of limitations in technique, the localization of BP in CNS myelin could not be unequivocally determined. In both PNS and CNS tissue, no anti-BP binding to nonmyelin cellular or membranous elements was detected.

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