Clinical evaluation of a new fully automated enzyme immunoassay for basophil histamine release in whole blood

2000 ◽  
Vol 49 (S1) ◽  
pp. 49-50 ◽  
Author(s):  
T. M. Li ◽  
K. J. Kontis ◽  
F. Rance ◽  
M. Abbal ◽  
D. A. Vuitton
Keyword(s):  
Histamine Release ◽  
Enzyme Immunoassay ◽  
Clinical Evaluation ◽  
Whole Blood

scholarly journals Fully Automated Enzyme Immunoassay System for the Determination of Activator-specific Histamine Release from Basophils in Whole Blood

1998 ◽  
Vol 44 (9) ◽  
pp. 2063-2065 ◽  
Author(s):  
Priscilla K Zia ◽  
Nasrin Namei ◽  
Ashok Patel ◽  
Kris J Kontis ◽  
Narayan Nayak ◽  
...  
Keyword(s):  
Histamine Release ◽  
Enzyme Immunoassay ◽  
Whole Blood

scholarly journals Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?

2017 ◽  
Vol 47 (4) ◽  
Author(s):  
Monique Paiva de Campos ◽  
Paula Mello De Luca ◽  
Alinne Rangel dos Santos Renzetti ◽  
Sara Maria Marques de Souza ◽  
Artur Augusto Velho Mendes Júnior ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Enzyme Immunoassay ◽  
Clinical Evaluation ◽  
Laboratory Tests ◽  
Endemic Area ◽  
Canine Visceral Leishmaniasis ◽  
Ministry Of Health ◽  
Evaluation Time ◽  
One Year ◽  
Serological Diagnostics

ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations of this dog were negative. Our results showed that in a non-endemic area, even at different evaluation times, dogs vaccinated against CVL with Leishmune® or Leish tec® did not seroconvert in the serological protocol used by the Brazilian Ministry of Health (DPP/EIE).

Radioreceptor Assay for Quantifying FK-506 Immunosuppressant in Whole Blood

1992 ◽  
Vol 38 (7) ◽  
pp. 1307-1310 ◽  
Author(s):  
J N Murthy ◽  
Y Chen ◽  
V S Warty ◽  
R Venkataramanan ◽  
J G Donnelly ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Whole Blood ◽  
Binding Protein ◽  
Radioreceptor Assay ◽  
Fk 506 ◽  
Blood Concentrations ◽  
Chromatographic Columns ◽  
Analytical Recovery

Abstract We describe a quantitative radioreceptor assay (RRA) for quantifying FK-506 in whole blood. FK-506 extracted from whole blood with a cyclohexyl-sorbent column competes with [3H]dihydro-FK-506 for binding to a partially purified preparation of FK-506 binding protein (FK-BP). Free and protein-bound FK-506 are separated on LH 20 Sephadex chromatographic columns. We compared the results of this method with those of an enzyme immunoassay that uses a monoclonal antibody: r = 0.97, Sy/x = 0.039. Between-day precisions (CV) at FK-506 concentrations of 8 and 17 micrograms/L were 9.2% and 8.2%, respectively. Within-run precisions were 5.9%, 8.1%, and 9.4%, respectively, at 4, 8, and 15 micrograms/L. Analytical recovery, evaluated at 5, 10, 15, 20, and 25 micrograms/L for FK-506 added to whole blood, ranged from 98% to 103%. The assay can reliably quantify FK-506 blood concentrations between 1.0 and 25 micrograms/L.

scholarly journals Clinical evaluation of whole blood prothrombin time (PT) and international normalized ratio (INR) using a Laser Speckle Rheology sensor

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Markandey M. Tripathi ◽  
Satoru Egawa ◽  
Alexandra G. Wirth ◽  
Diane M. Tshikudi ◽  
Elizabeth M. Van Cott ◽  
...  
Keyword(s):  
Prothrombin Time ◽  
Clinical Evaluation ◽  
Whole Blood ◽  
International Normalized Ratio ◽  
Laser Speckle

Allergen-Specific Histamine Release from Whole Blood in Flour-Sensitive Bakers

Respiration ◽  
1982 ◽  
Vol 43 (3) ◽  
pp. 208-220 ◽  
Author(s):  
H. Thiel ◽  
I. Zimmermann ◽  
B. Rasche ◽  
W.T. Ulmer
Keyword(s):  
Histamine Release ◽  
Whole Blood
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