The mechanisms of aquaporin control in the renal collecting duct

2008 ◽  
pp. 33-95 ◽  
Author(s):  
E. Klussmann ◽  
K. Maric ◽  
W. Rosenthal
Keyword(s):  
Collecting Duct ◽  
Renal Collecting Duct

scholarly journals The voltage‐dependent Cl − channel ClC‐5 and plasma membrane Cl − conductances of mouse renal collecting duct cells (mIMCD‐3)

2001 ◽  
Vol 536 (3) ◽  
pp. 769-783 ◽  
Author(s):  
J. A. Sayer ◽  
G. S. Stewart ◽  
S. H. Boese ◽  
M. A. Gray ◽  
S. H. S. Pearce ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Collecting Duct ◽  
Cl Channel ◽  
Duct Cells ◽  
Voltage Dependent ◽  
Collecting Duct Cells ◽  
Renal Collecting Duct

scholarly journals PD24-10 RENAL COLLECTING DUCT CARCINOMA: CLINICAL PRESENTATION AND PATTERNS OF CARE

2018 ◽  
Vol 199 (4S) ◽  
Author(s):  
Zain Hyder ◽  
Michael Callegari ◽  
Daniel Parker ◽  
Michael Cookson ◽  
Brian Cross ◽  
...  
Keyword(s):  
Clinical Presentation ◽  
Collecting Duct ◽  
Patterns Of Care ◽  
Duct Carcinoma ◽  
Collecting Duct Carcinoma ◽  
Renal Collecting Duct

scholarly journals Interaction between Epithelial Sodium Channel γ-Subunit and Claudin-8 Modulates Paracellular Sodium Permeability in Renal Collecting Duct

2020 ◽  
Vol 31 (5) ◽  
pp. 1009-1023 ◽  
Author(s):  
Ali Sassi ◽  
Yubao Wang ◽  
Alexandra Chassot ◽  
Olga Komarynets ◽  
Isabelle Roth ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Knockout Mice ◽  
Collecting Duct ◽  
Epithelial Sodium Channel ◽  
Paracellular Permeability ◽  
Sodium Reabsorption ◽  
Kidney Tubule ◽  
Sodium Permeability ◽  
Tubular Lumen ◽  
Renal Collecting Duct

BackgroundWater and solute transport across epithelia can occur via the transcellular or paracellular pathways. Tight junctions play a key role in mediating paracellular ion reabsorption in the kidney. In the renal collecting duct, which is a typical absorptive tight epithelium, coordination between transcellular sodium reabsorption and paracellular permeability may prevent the backflow of reabsorbed sodium to the tubular lumen along a steep electrochemical gradient.MethodsTo investigate whether transcellular sodium transport controls tight-junction composition and paracellular permeability via modulating expression of the transmembrane protein claudin-8, we used cultured mouse cortical collecting duct cells to see how overexpression or silencing of epithelial sodium channel (ENaC) subunits and claudin-8 affect paracellular permeability. We also used conditional kidney tubule–specific knockout mice lacking ENaC subunits to assess the ENaC’s effect on claudin-8 expression.ResultsOverexpression or silencing of the ENaC γ-subunit was associated with parallel and specific changes in claudin-8 abundance. Increased claudin-8 abundance was associated with a reduction in paracellular permeability to sodium, whereas decreased claudin-8 abundance was associated with the opposite effect. Claudin-8 overexpression and silencing reproduced these functional effects on paracellular ion permeability. Conditional kidney tubule–specific ENaC γ-subunit knockout mice displayed decreased claudin-8 expression, confirming the cell culture experiments' findings. Importantly, ENaC β-subunit or α-subunit silencing or kidney tubule–specific β-ENaC or α-ENaC knockout mice did not alter claudin-8 abundance.ConclusionsOur data reveal the specific coupling between ENaC γ-subunit and claudin-8 expression. This coupling may play an important role in preventing the backflow of reabsorbed solutes and water to the tubular lumen, as well as in coupling paracellular and transcellular sodium permeability.

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