NMR study of diffusion in protein hydration shells

2007 ◽  
pp. 211-215 ◽  
Author(s):  
K. Kotitschke ◽  
R. Kimmich ◽  
E. Rommel ◽  
F. Parak
Keyword(s):  
Protein Hydration ◽  
Hydration Shells ◽  
Nmr Study

scholarly journals Thermal contraction of aqueous glycerol and ethylene glycol solutions for optimized protein-crystal cryoprotection

2016 ◽  
Vol 72 (6) ◽  
pp. 742-752 ◽  
Author(s):  
Chen Shen ◽  
Ethan F. Julius ◽  
Timothy J. Tyree ◽  
David W. Moreau ◽  
Hakan Atakisi ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Practical Importance ◽  
Bulk Water ◽  
Protein Crystal ◽  
Protein Hydration ◽  
Hydration Water ◽  
Thermal Contraction ◽  
Cryogenic Temperatures ◽  
Hydration Shells ◽  
Protein Molecules

The thermal contraction of aqueous cryoprotectant solutions on cooling to cryogenic temperatures is of practical importance in protein cryocrystallography and in biological cryopreservation. In the former case, differential contraction on cooling of protein molecules and their lattice relative to that of the internal and surrounding solvent may lead to crystal damage and the degradation of crystal diffraction properties. Here, the amorphous phase densities of aqueous solutions of glycerol and ethylene glycol atT= 77 K have been determined. Densities with accuracies of <0.5% to concentrations as low as 30%(w/v) were determined by rapidly cooling drops with volumes as small as 70 pl, assessing their optical clarity and measuring their buoyancy in liquid nitrogen–argon solutions. The use of these densities in contraction matching of internal solvent to the available solvent spaces is complicated by several factors, most notably the exclusion of cryoprotectants from protein hydration shells and the expected deviation of the contraction behavior of hydration water from bulk water. The present methods and results will assist in developing rational approaches to cryoprotection and an understanding of solvent behavior in protein crystals.

Measurement of protein hydration shells using a quartz microbalance

1989 ◽  
Vol 63 (16) ◽  
pp. 1743-1746 ◽  
Author(s):  
L. Reinisch ◽  
R. D. Kaiser ◽  
J. Krim
Keyword(s):  
Protein Hydration ◽  
Quartz Microbalance ◽  
Hydration Shells

Dipolar susceptibility of protein hydration shells

2018 ◽  
Vol 713 ◽  
pp. 210-214 ◽  
Author(s):  
Salman Seyedi ◽  
Dmitry V. Matyushov
Keyword(s):  
Protein Hydration ◽  
Hydration Shells

scholarly journals Dipolar Nanodomains in Protein Hydration Shells

2015 ◽  
Vol 6 (3) ◽  
pp. 407-412 ◽  
Author(s):  
Daniel R. Martin ◽  
Dmitry V. Matyushov
Keyword(s):  
Protein Hydration ◽  
Hydration Shells

Arylation of enelactams using TIPSOTf: reaction scope and mechanistic insight

2021 ◽  
Author(s):  
Tomasz J. Idzik ◽  
Zofia M. Myk ◽  
Łukasz Struk ◽  
Magdalena Perużyńska ◽  
Gabriela Maciejewska ◽  
...  
Keyword(s):  
Reaction Mechanism ◽  
Multinuclear Nmr ◽  
Mechanistic Insight ◽  
Nmr Study ◽  
Wide Range

Triisopropylsilyltrifluoromethanesulfonate can be effectively used for the arylation of a wide range of enelactams. The multinuclear NMR study provided deep insights into the reaction mechanism.

A NMR study of human β2 microglobulin

1981 ◽  
Vol 78 ◽  
pp. 837-841 ◽  
Author(s):  
Marcel Sarrazin ◽  
Claudette Briand ◽  
Madeleine Bourdeaux ◽  
Michèle Chauvet ◽  
Claude Vincent ◽  
...  
Keyword(s):  
Β2 Microglobulin ◽  
Nmr Study
Sign in / Sign up

Export Citation Format

Share Document