Determination of the molar mass of pigment-containing complexes of intrinsic membrane proteins: Problems, solutions and application to the light-harvesting complex B800/820 of Rhodospirillum molischianum

2007 ◽  
pp. 14-19 ◽  
Author(s):  
D. Schubert ◽  
C. Tziatzios ◽  
J. A. Broek ◽  
P. Schuck ◽  
L. Germeroth ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Molar Mass ◽  
Light Harvesting ◽  
Light Harvesting Complex ◽  
Rhodospirillum Molischianum

Overexpression of integral membrane proteins for structural studies

1995 ◽  
Vol 28 (3) ◽  
pp. 315-422 ◽  
Author(s):  
R. Grisshammer ◽  
C. G. Tateu
Keyword(s):  
Membrane Proteins ◽  
Light Harvesting ◽  
Atomic Level ◽  
Integral Membrane Proteins ◽  
Biological Processes ◽  
Structural Studies ◽  
Light Harvesting Complex ◽  
Prostaglandin H ◽  
2D Crystals

Determination of the structure of integral membrane proteins is a challenging task that is essential to understand how fundamental biological processes (such as photosynthesis, respiration and solute translocation) function at the atomic level. Crystallisation of membrane proteins in 3D has led to the determination of four atomic resolution structures [photosynthetic reaction centres (Allenet al. 1987; Changet al. 1991; Deisenhofer & Michel, 1989; Ermleret al. 1994); porins (Cowanet al. 1992; Schirmeret al. 1995; Weisset al. 1991); prostaglandin H2synthase (Picotet al. 1994); light harvesting complex (McDermottet al. 1995)], and crystals of membrane proteins formed in the plane of the lipid bilayer (2D crystals) have produced two more structures [bacteriorhodopsin (Hendersonet al. 1990); light harvesting complex (Kühlbrandtet al. 1994)].

Capillary electrophoresis of closely related intrinsic thylakoid membrane proteins of the photosystem II light-harvesting complex (LHC II)

1996 ◽  
Vol 17 (10) ◽  
pp. 1597-1601 ◽  
Author(s):  
Lello Zolla ◽  
Maria Bianchetti ◽  
Anna Maria Timperio ◽  
Giuseppe Scarascia Mugnozza ◽  
Danilo Corradini
Keyword(s):  
Capillary Electrophoresis ◽  
Membrane Proteins ◽  
Photosystem Ii ◽  
Thylakoid Membrane ◽  
Light Harvesting ◽  
Lhc Ii ◽  
Light Harvesting Complex ◽  
Thylakoid Membrane Proteins
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