Sedimentation velocity analysis of the DNA-protamine interaction

2007 ◽  
pp. 36-40 ◽  
Author(s):  
S. Bickhardt ◽  
-G. Ebert ◽  
N. Nishi ◽  
K. Hagiwara
Keyword(s):  
Sedimentation Velocity ◽  
Velocity Analysis

scholarly journals The structure of L-lactate oxidase from Mycobacterium smegmatis

1977 ◽  
Vol 165 (2) ◽  
pp. 375-383 ◽  
Author(s):  
P A Sullivan ◽  
C Y Soon ◽  
W J Schreurs ◽  
J F Cutfield ◽  
M G Shepherd
Keyword(s):  
Electron Microscopy ◽  
Mycobacterium Smegmatis ◽  
Sedimentation Velocity ◽  
Equilibrium Analysis ◽  
Sedimentation Equilibrium ◽  
Cross Linking ◽  
Velocity Analysis ◽  
Approach To Equilibrium ◽  
Lactate Oxidase ◽  
Dimethyl Suberimidate

1. An improved purification was developed for L-lactate oxidase from Mycobacterium smegmatis. 2. The mol.wt. of the native enzyme by a sedimentation-equilibrium analysis was 345 000, and other ultracentrifuge methods gave values in the range 345 000-350 000. 3. An amino acid analysis, determinations of protein and flavin, a sedimentation-velocity analysis and an approach to equilibrium analysis gave values for the subunit mol.wt. in the range 43 500-47 000. 4. It was concluded that L-lactate oxidase contains eight subunits of mol.wt. 43 500. 5. Cross-linking of the subunits with dimethyl suberimidate and electron-microscopy studies were consistent with an octameric structure.

scholarly journals Sedimentation Velocity Analysis with Fluorescence Detection of Mutant Huntingtin Exon 1 Aggregation inDrosophila melanogasterandCaenorhabditis elegans

Biochemistry ◽  
2017 ◽  
Vol 56 (35) ◽  
pp. 4676-4688 ◽  
Author(s):  
Surin A. Kim ◽  
Victoria F. D’Acunto ◽  
Bashkim Kokona ◽  
Jennifer Hofmann ◽  
Nicole R. Cunningham ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Sedimentation Velocity ◽  
Velocity Analysis ◽  
Mutant Huntingtin ◽  
Exon 1

scholarly journals Sedimentation velocity analysis of TMPyP4-induced dimer formation of human telomeric G-quadruplex

RSC Advances ◽  
2017 ◽  
Vol 7 (87) ◽  
pp. 55098-55105 ◽  
Author(s):  
Yating Gao ◽  
Tianlei Guang ◽  
Xiaodong Ye
Keyword(s):  
Analytical Ultracentrifugation ◽  
Sedimentation Velocity ◽  
Velocity Analysis ◽  
Dimer Formation ◽  
G Quadruplex

Analytical ultracentrifugation sedimentation velocity (AUC-SV) was used to study the interactions between TMPyP4 and AGGG(TTAGGG)3 (Tel22) and the TMPyP4-induced dimer formation of G-quadruplex.

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