Output-sensitive cell enumeration in hyperplane arrangements

1998 ◽  
pp. 300-309 ◽  
Author(s):  
Nora Sleumer
Keyword(s):  
Hyperplane Arrangements ◽  
Sensitive Cell ◽  
Cell Enumeration

scholarly journals Primary Cultures of Embryonic Chicken Neurons for Sensitive Cell-Based Assay of Botulinum Neurotoxin: Implications for Therapeutic Discovery

2007 ◽  
Vol 12 (3) ◽  
pp. 370-377 ◽  
Author(s):  
Andrea M. Stahl ◽  
Gordon Ruthel ◽  
Edna Torres-Melendez ◽  
Tara A. Kenny ◽  
Rekha G. Panchal ◽  
...  
Keyword(s):  
Nervous System ◽  
Botulinum Toxin ◽  
Neutralizing Antibodies ◽  
Botulinum Neurotoxin ◽  
Potent Inhibitor ◽  
Primary Cultures ◽  
Embryonic Chick ◽  
Sensitive Cell ◽  
Botulinum Neurotoxin A ◽  
Therapeutic Compounds

Botulinum toxin is an exceedingly potent inhibitor of neurotransmission across the neuromuscular junction, causing flaccid paralysis and death. The potential for misuse of this deadly poison as a bioweapon has added a greater urgency to the search for effective therapeutics. The development of sensitive and efficient cell-based assays for the evaluation of toxin antagonists is crucial to the rapid and successful identification of therapeutic compounds. The authors evaluated the sensitivity of primary cultures from 4 distinct regions of the embryonic chick nervous system to botulinum neurotoxin A (BoNT/A) cleavage of synaptosomal-associated protein of 25 kD (SNAP-25). Although differences in sensitivity were apparent, SNAP-25 cleavage was detectable in neuronal cells from each of the 4 regions within 3 h at BoNT/A concentrations of 1 nM or lower. Co-incubation of chick neurons with BoNT/A and toxin-neutralizing antibodies inhibited SNAP-25 cleavage, demonstrating the utility of these cultures for the assay of BoNT/A antagonists. ( Journal of Biomolecular Screening 2007:370-377)

Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection

2018 ◽  
Vol 10 (7) ◽  
pp. 6618-6623 ◽  
Author(s):  
Shanshan Liu ◽  
Ping He ◽  
Sameer Hussain ◽  
Huan Lu ◽  
Xin Zhou ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Cell Detection ◽  
Sensitive Cell ◽  
Turn On

scholarly journals Phytochemical Analysis and In Vitro Effects of Allium fistulosum L. and Allium sativum L. Extracts on Human Normal and Tumor Cell Lines: A Comparative Study

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 574
Author(s):  
Adrian Bogdan Țigu ◽  
Cristian Silviu Moldovan ◽  
Vlad-Alexandru Toma ◽  
Anca Daniela Farcaș ◽  
Augustin Cătălin Moț ◽  
...  
Keyword(s):  
Cell Line ◽  
Allium Sativum ◽  
Allium Fistulosum ◽  
Tumor Cell Lines ◽  
Sensitive Cell ◽  
Sensitive Cell Line ◽  
Welsh Onion ◽  
Normal Cells ◽  
Ic50 Value ◽  
High Concentrations

Allium sativum L. (garlic bulbs) and Allium fistulosum L. (Welsh onion leaves) showed quantitative differences of identified compounds: allicin and alliin (380 µg/mL and 1410 µg/mL in garlic; 20 µg/mL and 145 µg/mL in Welsh onion), and the phenolic compounds (chlorogenic acid, p-coumaric acid, ferulic acid, gentisic acid, 4-hydroxybenzoic acid, kaempferol, isoquercitrin, quercitrin, quercetin, and rutin). The chemical composition determined the inhibitory activity of Allium extracts in a dose-dependent manner, on human normal cells (BJ-IC50 0.8841% garlic/0.2433% Welsh onion and HaCaT-IC50 1.086% garlic/0.6197% Welsh onion) and tumor cells (DLD-1-IC50 5.482%/2.124%; MDA-MB-231-IC50 6.375%/2.464%; MCF-7-IC50 6.131%/3.353%; and SK-MES-1-IC50 4.651%/5.819%). At high concentrations, the cytotoxic activity of each extract, on normal cells, was confirmed by: the 50% of the growth inhibition concentration (IC50) value, the cell death induced by necrosis, and biochemical determination of LDH, catalase, and Caspase-3. The four tumor cell lines treated with high concentrations (10%, 5%, 2.5%, and 1.25%) of garlic extract showed different sensibility, appreciated on the base of IC50 value for the most sensitive cell line (SK-MES-1), and the less sensitive (MDA-MB-231) cell line. The high concentrations of Welsh onion extract (5%, 2.5%, and 1.25%) induced pH changes in the culture medium and SK-MES-1 being the less sensitive cell line.

scholarly journals On polytopes and generalizations of the KLT relations

2020 ◽  
Vol 2020 (12) ◽  
Author(s):  
Nikhil Kalyanapuram
Keyword(s):  
Scattering Amplitudes ◽  
Large Class ◽  
Moduli Space ◽  
Differential Forms ◽  
Natural Generalization ◽  
Intersection Theory ◽  
Hyperplane Arrangements ◽  
Number Of Solutions ◽  
Scalar Theories ◽  
Double Copy

Abstract We combine the technology of the theory of polytopes and twisted intersection theory to derive a large class of double copy relations that generalize the classical relations due to Kawai, Lewellen and Tye (KLT). To do this, we first study a generalization of the scattering equations of Cachazo, He and Yuan. While the scattering equations were defined on ℳ0, n — the moduli space of marked Riemann spheres — the new scattering equations are defined on polytopes known as accordiohedra, realized as hyperplane arrangements. These polytopes encode as patterns of intersection the scattering amplitudes of generic scalar theories. The twisted period relations of such intersection numbers provide a vast generalization of the KLT relations. Differential forms dual to the bounded chambers of the hyperplane arrangements furnish a natural generalization of the Bern-Carrasco-Johansson (BCJ) basis, the number of which can be determined by counting the number of solutions of the generalized scattering equations. In this work the focus is on a generalization of the BCJ expansion to generic scalar theories, although we use the labels KLT and BCJ interchangeably.

scholarly journals The drop plate method as an alternative for Azospirillum spp viable cell enumeration within the consensus protocol of the REDCAI network

2021 ◽  
Author(s):  
Luciana P. Di Salvo ◽  
Julia E. García ◽  
Mariana L. Puente ◽  
Josefina Amigo ◽  
Analía Anríquez ◽  
...  
Keyword(s):  
Viable Cell ◽  
Consensus Protocol ◽  
Plate Method ◽  
Cell Enumeration ◽  
Drop Plate
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