Adaptation of mammalian skeletal muscle fibers to chronic electrical stimulation

1992 ◽  
pp. 115-202 ◽  
Author(s):  
Dirk Pette ◽  
Gerta Vrbová
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fibers ◽  
Mammalian Skeletal Muscle ◽  
Skeletal Muscle Fibers ◽  
Chronic Electrical Stimulation

The “KIMWIPE” Effect in Ultra-Thin Cryosections

1985 ◽  
Vol 43 ◽  
pp. 458-459
Author(s):  
I. Taylor ◽  
P. Ingram ◽  
J.R. Sommer
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fibers ◽  
Ice Crystals ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Thin Sections ◽  
Skeletal Muscle Fibers ◽  
Freeze Dried ◽  
Ice Crystal Formation

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).

The Adaptive Potential of Skeletal Muscle Fibers

2002 ◽  
Vol 27 (4) ◽  
pp. 423-448 ◽  
Author(s):  
Dirk Pette
Keyword(s):  
Skeletal Muscle ◽  
Fiber Type ◽  
Muscle Fibers ◽  
Protein Isoforms ◽  
Mammalian Skeletal Muscle ◽  
Adaptive Potential ◽  
Hybrid Fibers ◽  
Neuromuscular Activity ◽  
Skeletal Muscle Fibers ◽  
Initial Location

Mammalian skeletal muscle fibers display a great adaptive potential. This potential results from the ability of muscle fibers to adjust their molecular, functional, and metabolic properties in response to altered functional demands, such as changes in neuromuscular activity or mechanical loading. Adaptive changes in the expression of myofibrillar and other protein isoforms result in fiber type transitions. These transitions occur in a sequential order and encompass a spectrum of pure and hybrid fibers. Depending on the quality, intensity, and duration of the alterations in functional demand, muscle fibers may undergo functional transitions in the direction of slow or fast, as well as metabolic transitions in the direction of aerobic-oxidative or glycotytic. The maximum range of possible transitions in either direction depends on the fiber phenotype and is determined by its initial location in the fiber spectrum. Key words: Ca-sequestering proteins, energy metabolism, fiber type transition, myofibrillar protein isofonns, myosin, neuromuscular activity

scholarly journals Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers

2001 ◽  
Vol 155 (1) ◽  
pp. 27-40 ◽  
Author(s):  
Yewei Liu ◽  
Zoltán Cseresnyés ◽  
William R. Randall ◽  
Martin F. Schneider
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Nuclear Translocation ◽  
Fiber Type ◽  
Muscle Fibers ◽  
Adult Skeletal Muscle ◽  
Skeletal Muscle Fibers ◽  
Slow Twitch ◽  
Nuclear Foci ◽  
Fast Twitch

TTranscription factor nuclear factor of activated T cells NFATc (NFATc1, NFAT2) may contribute to slow-twitch skeletal muscle fiber type–specific gene expression. Green fluorescence protein (GFP) or FLAG fusion proteins of either wild-type or constitutively active mutant NFATc [NFATc(S→A)] were expressed in cultured adult mouse skeletal muscle fibers from flexor digitorum brevis (predominantly fast-twitch). Unstimulated fibers expressing NFATc(S→A) exhibited a distinct intranuclear pattern of NFATc foci. In unstimulated fibers expressing NFATc–GFP, fluorescence was localized at the sarcomeric z-lines and absent from nuclei. Electrical stimulation using activity patterns typical of slow-twitch muscle, either continuously at 10 Hz or in 5-s trains at 10 Hz every 50 s, caused cyclosporin A–sensitive appearance of fluorescent foci of NFATc–GFP in all nuclei. Fluorescence of nuclear foci increased during the first hour of stimulation and then remained constant during a second hour of stimulation. Kinase inhibitors and ionomycin caused appearance of nuclear foci of NFATc–GFP without electrical stimulation. Nuclear translocation of NFATc–GFP did not occur with either continuous 1 Hz stimulation or with the fast-twitch fiber activity pattern of 0.1-s trains at 50 Hz every 50 s. The stimulation pattern–dependent nuclear translocation of NFATc demonstrated here could thus contribute to fast-twitch to slow-twitch fiber type transformation.

scholarly journals Calcium Sparklets in Intact Mammalian Skeletal Muscle Fibers Expressing the Embryonic CaV1.1 Splice Variant

2015 ◽  
Vol 108 (2) ◽  
pp. 504a
Author(s):  
Beatrix Dienes ◽  
Nasreen Sultana ◽  
Janos Vincze ◽  
Monika Sztretye ◽  
Peter Szentesi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Splice Variant ◽  
Muscle Fibers ◽  
Mammalian Skeletal Muscle ◽  
Skeletal Muscle Fibers

scholarly journals Factors affecting SOCE activation in mammalian skeletal muscle fibers

2009 ◽  
Vol 59 (4) ◽  
pp. 317-328 ◽  
Author(s):  
Pura Bolaños ◽  
Alis Guillén ◽  
Reinaldo DiPolo ◽  
Carlo Caputo
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Mammalian Skeletal Muscle ◽  
Factors Affecting ◽  
Skeletal Muscle Fibers

Differential Effects of S(-)-Ropivacaine and Bupivacaine on Intracellular Ca2+ Homeostasis in Mammalian Skeletal Muscle Fibers

2002 ◽  
Vol 96 (Sup 2) ◽  
pp. A972 ◽  
Author(s):  
Wolfgang Zink ◽  
Gudrun Kunst ◽  
Eike Martin ◽  
Rainer H.A. Fink ◽  
Bernhard M. Graf
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Mammalian Skeletal Muscle ◽  
Differential Effects ◽  
Skeletal Muscle Fibers

Bupivacaine, but Not Ropivacaine Induces Apoptosis in Mammalian Skeletal Muscle Fibers

2002 ◽  
Vol 96 (Sup 2) ◽  
pp. A971 ◽  
Author(s):  
Wolfgang Zink ◽  
Christoph Seif ◽  
Juergen R.E. Bohl ◽  
Eike Martin ◽  
Bernhard M. Graf
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Mammalian Skeletal Muscle ◽  
Skeletal Muscle Fibers

scholarly journals Inward Rectifier Potassium Channels in Mammalian Skeletal Muscle Fibers

2014 ◽  
Vol 106 (2) ◽  
pp. 129a
Author(s):  
Julio L. Vergara ◽  
Marbella Quiñonez ◽  
Carl Yu ◽  
Marino DiFranco
Keyword(s):  
Skeletal Muscle ◽  
Potassium Channels ◽  
Muscle Fibers ◽  
Inward Rectifier ◽  
Mammalian Skeletal Muscle ◽  
Inward Rectifier Potassium Channels ◽  
Skeletal Muscle Fibers
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