Structural analysis at molecular dimensions of proteins and protein assemblies using Electron Microscopy (EM) and image processing

Author(s):  
Uell Aebi
2002 ◽  
Vol 387 (1) ◽  
pp. 145-150 ◽  
Author(s):  
Kyoichi Oshida ◽  
Kozo Osawa ◽  
Tatsuo Nakazawa ◽  
Yasuo Fukai ◽  
Takuya Hayashi ◽  
...  

2013 ◽  
Vol 275 ◽  
pp. 409-412 ◽  
Author(s):  
K. Oshida ◽  
M. Murata ◽  
K. Fujiwara ◽  
T. Itaya ◽  
T. Yanagisawa ◽  
...  

2011 ◽  
Vol 67 (a1) ◽  
pp. C229-C230
Author(s):  
M. Serna ◽  
J. Martin-Benito ◽  
G. Carranz ◽  
R. Janowski ◽  
R. Castaño ◽  
...  

2008 ◽  
Vol 379 (1) ◽  
pp. 174-187 ◽  
Author(s):  
Osman Salih ◽  
Han Remaut ◽  
Gabriel Waksman ◽  
Elena V. Orlova

Author(s):  
R.W. Horne

The technique of surrounding virus particles with a neutralised electron dense stain was described at the Fourth International Congress on Electron Microscopy, Berlin 1958 (see Home & Brenner, 1960, p. 625). For many years the negative staining technique in one form or another, has been applied to a wide range of biological materials. However, the full potential of the method has only recently been explored following the development and applications of optical diffraction and computer image analytical techniques to electron micrographs (cf. De Hosier & Klug, 1968; Markham 1968; Crowther et al., 1970; Home & Markham, 1973; Klug & Berger, 1974; Crowther & Klug, 1975). These image processing procedures have allowed a more precise and quantitative approach to be made concerning the interpretation, measurement and reconstruction of repeating features in certain biological systems.


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