Effect of Cold Pretreatment and Incubation Temperature on Bread Wheat (Triticum aestivum L.) Anther Culture

2001 ◽  
Vol 29 (3-4) ◽  
pp. 331-338 ◽  
Author(s):  
I. N. Xynias ◽  
I. A. Zamani ◽  
E. Gouli-Vavdinoudi ◽  
D. G. Roupakias
Genetika ◽  
2019 ◽  
Vol 51 (2) ◽  
pp. 447-461
Author(s):  
Imren Kutlu ◽  
Zeynep Sirel ◽  
Ozcan Yorgancilar ◽  
Aysel Yorgancilar

1990 ◽  
Vol 70 (1) ◽  
pp. 183-191 ◽  
Author(s):  
LINDA J. McGREGOR ◽  
ALAN McHUGHEN

Four spring wheat cultivars (Katepwa, Columbus, Chris and Israel 676) were compared for androgenic ability. Temperature, medium, agar concentration and cold pretreatment were examined to find the optimal combinations for anther culture of Katepwa and Columbus in particular. At an incubation temperature of 30 °C, anthers of Chris were significantly more responsive than the other genotypes. In three temperature treatments (26, 28 and 30 °C) the potato extract medium (P1) was superior to the synthetic medium (N6) (Chu 1978) for callus induction and green plant regeneration. Over all genotypes, liquid media provided greater anther response than either 0.8% or 1.8% agar. At 28 °C, cultured anthers showed no significant increase in callus formation after being subjected to a cold pretreatment. A cold pretreatment prior to culture at 30 °C elicited a significant decrease in callus formation as compared with no cold pretreatment. However, maximum callus induction and regeneration frequencies for Katepwa and Columbus were obtained at 28 °C in the P1 liquid medium with cold pretreatment. An incubation temperature of 26 °C was inferior to 28° and 30 °C for callus induction and regeneration.Key words: Anther culture, wheat, genotype, incubation temperature, media, agar concentration


2021 ◽  
Vol 20 (5) ◽  
pp. 1180-1192
Author(s):  
Meng-jiao YANG ◽  
Cai-rong WANG ◽  
Muhammad Adeel HASSAN ◽  
Yu-ying WU ◽  
Xian-chun XIA ◽  
...  

2010 ◽  
Vol 121 (5) ◽  
pp. 941-950 ◽  
Author(s):  
Hao Bing Li ◽  
Guo Qiang Xie ◽  
Jun Ma ◽  
Gui Ru Liu ◽  
Shu Min Wen ◽  
...  

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).


2005 ◽  
Vol 6 (1) ◽  
pp. 81-81
Author(s):  
Rosy Raman ◽  
Harsh Raman ◽  
Katie Johnstone ◽  
Chris Lisle ◽  
Alison Smith ◽  
...  

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