Corticotropin-releasing factor (CRF) system localization in human fetal heart

HORMONES ◽  
2016 ◽  
Vol 15 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Efterpi Chouridou ◽  
Maria Lambropoulou ◽  
Maria Koureta ◽  
Christina Zarouchlioti ◽  
Ioanna Balgouranidou ◽  
...  
Keyword(s):  
Fetal Heart ◽  
Corticotropin Releasing Factor ◽  
Human Fetal Heart ◽  
Crf System

scholarly journals Corticotropin-releasing factor (CRF) system localization in human fetal heart

HORMONES ◽  
2016 ◽  
Author(s):  
Efterpi Chouridou ◽  
Maria Lambropoulou ◽  
Maria Koureta ◽  
Christina Zarouchlioti ◽  
Ioanna Balgouranidou ◽  
...  
Keyword(s):  
Fetal Heart ◽  
Corticotropin Releasing Factor ◽  
Human Fetal Heart ◽  
Crf System

Expression of the Atrial Natriuretic Peptide Gene in Human Fetal Heart*

1989 ◽  
Vol 69 (4) ◽  
pp. 729-737 ◽  
Author(s):  
D. G. GARDNER ◽  
B. K. HEDGES ◽  
J. WU ◽  
M. C. LAPOINTE ◽  
C. F. DESCHEPPER
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Fetal Heart ◽  
Human Fetal Heart ◽  
Peptide Gene ◽  
Atrial Natriuretic

scholarly journals Corticotropin-Releasing Factor Inhibits Luteinizing Hormone-Stimulated P450c17 Gene Expression and Androgen Production by Isolated Thecal Cells of Human Ovarian Follicles1

1998 ◽  
Vol 83 (2) ◽  
pp. 448-452
Author(s):  
H. F. Erden ◽  
I. H. Zwain ◽  
H. Asakura ◽  
S. S. C. Yen
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Inhibitory Effect ◽  
Corticotropin Releasing Factor ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Messenger Ribonucleic Acid ◽  
Estrogen Production ◽  
Thecal Cells ◽  
Crf System

Recently, we reported that the thecal compartment of the human ovary contains a CRF system replete with gene expression and protein for corticotropin-releasing factor (CRF), CRF-Receptor 1 (CRF-R1), and the blood-derived high affinity CRF-binding protein (CRF-BP). Granulosa cells are devoid of the CRF system. The parallel increases in intensity of CRF, CRF-R1, and 17α-hydroxylase messenger ribonucleic acid (mRNA) and proteins in thecal cells with follicular maturation suggest that the intraovarian CRF system may play an autocrine role regulating androgen biosynthesis, with a downstream effect on estrogen production by granulosa cells. The functionality of the ovarian CRF system may be conditioned by the relative presence of plasma-derived CRF-BP by virtue of its localization of protein, but not transcript in thecal cells and its ability to compete with CRF for the CRF receptor. To further these findings, in the present study we have examined the effect of CRF on LH-stimulated 17α-hydroxylase (P450c17) gene expression and androgen production by isolated thecal cells from human ovarian follicles (11–13 mm). During the 48-h culture, addition of LH (10 ng/mL) to the medium increased by 5- and 6-fold dehydroepiandrosterone and androstenedione production by thecal cells. Remarkably, the LH-stimulated, but not basal, androgen production was inhibited by CRF in a time- and dose-dependent manner. The half-maximal (ID50) effect dose of CRF occurred at 5 × 10−8 mol/L, and at a maximal concentration of 10−6 mol/L, CRF completely inhibited LH-stimulated androgen production. This inhibitory effect of CRF became evident at 12 h (45%), and by 24 h the effect was more pronounced, with a 70% reduction from baseline. As determined by Northern analyses, CRF dose dependently decreased LH-stimulated P450c17 mRNA levels, with a maximal inhibition of 85% P450c17 gene expression at a CRF concentration of 10−6 mol/L. With the addition of 10−6 mol/L of the antagonist α-helical CRF-(9–41), the inhibitory effect of CRF was partially reversed for both P450c17 mRNA (75%) and androgen production (50%), indicating the CRF-R1-mediated event. In conclusion, the present study demonstrated a potent inhibitory effect of CRF on LH-stimulated dehydroepiandrosterone and androstenedione production that appears to be mediated through the reduction of P450c17 gene expression. Thus, the ovarian CRF system may function as autocrine regulators for androgen biosynthesis in the thecal cell compartment to maintain optimal substrate for estrogen biosynthesis by granulosa cells. Further studies to define the role of CRF-BP in the endocrine modulation of the intraovarian CRF system are needed.

The Paraventriculo-Infundibular Corticotropin Releasing Factor (CRF)-System II. Functional Aspects

1985 ◽  
pp. 21-37 ◽  
Author(s):  
István Lengvári ◽  
Magdolna Kovács ◽  
Zsolt Liposits ◽  
Sándor Vigh ◽  
Béla Flerkó
Keyword(s):  
Corticotropin Releasing Factor ◽  
Functional Aspects ◽  
Crf System

scholarly journals Endothelial Growth Factor Receptors in Human Fetal Heart

Circulation ◽  
1999 ◽  
Vol 100 (6) ◽  
pp. 583-586 ◽  
Author(s):  
Taina A. Partanen ◽  
Taija Makinen ◽  
Johanna Arola ◽  
Toshio Suda ◽  
Herbert A. Weich ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fetal Heart ◽  
Growth Factor Receptors ◽  
Human Fetal Heart ◽  
Endothelial Growth Factor

scholarly journals Distinct expression patterns of connexins in the chambers and conduction system of the human fetal heart

2002 ◽  
Vol 39 ◽  
pp. 229
Author(s):  
Riyaz A. Kaba ◽  
Steven R. Coppen ◽  
Emmanuel Dupont ◽  
Jeremy N. Skepper ◽  
Suzy Elneit ◽  
...  
Keyword(s):  
Fetal Heart ◽  
Expression Patterns ◽  
Conduction System ◽  
Human Fetal Heart

The Effect of Neuromuscular Blockade on Human Fetal Heart Rate and Its Variation

1994 ◽  
Vol 38 (5) ◽  
pp. 271
Author(s):  
JOHN A. D. SPENCER ◽  
GREG RYAN ◽  
DANIEL RONDEROS-DUMIT ◽  
UMBERTO NICOLINI ◽  
CHARLES H. RODECK
Keyword(s):  
Heart Rate ◽  
Neuromuscular Blockade ◽  
Fetal Heart Rate ◽  
Fetal Heart ◽  
Human Fetal Heart
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